Wako Product Information
<For Research Use Only>
Wako Catalog No. 010-18551(1 g)
016-18553(250 mg)
Keep at 2 -10 degrees C
beta-Amylase, from Barley


Assay
< Principle >
Starch + H2O -(beta-Amylase)-> Maltose

< Reagents >

< Procedure >
Reagent
Test
Blank
C
1 mL
1 mL
A
0.8 mL
0.8 mL
B
-
2.0 mL
D
0.2 mL
-
Incubate for exactly 3.0 min. at 25C.
B
2 mL
-
D
-
0.2 mL
Place in a boiling water bath for 5 min.,
then cool on ice.
Water
46 mL
46 mL
Record the A490nm for both the Test
and Blank using water as control.
Reagent
Std 1
Std 2
Std 3
Blank
E
0.1 mL
0.2 mL
0.3 mL
-
A
0.9 mL
0.8 mL
0.7 mL
1 mL
C
1 mL
1 mL
1 mL
1 mL
B
2 mL
2 mL
2 mL
2 mL
Place in a boiling water bath for 5 min.,
then cool on ice.
Water
46 mL
46 mL
46 mL
46 mL
Record the A490nm for both the Test
and Blank using water as control
and make the standard curve.

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