Laboratory Chemicals Division > Product Information > A > Anti Human Tenascin-C

Research for Cancer and Organogenesis
Anti Human Tenascin-C, Rat Monoclonal Antibody

Leaflet(192kb/1p)

Tenascin is the glycoprotein, which is one of the extracellular matrix, and Tenascin-C, -X, -R, -W and -Y are known. Tenascin exists in the interstice of epithelia and mesenchyme on organogenesis early stage. Additionally, Tenascin is produced in cancer cells and the process of the wound healing. Therefore, the possibility that tenascin is important as the cancer marker is thought.
This product is Anti Human Tenascin-C, monoclonal antibody, which is applicable to research for cancer and other disorders, organogenesis and rebuilding of body tissue.

Product Outline

• Antigen: Tenascin-C purified from the GIT medium culture supernatant of human myeloma cells A375
• Purification: Purified by Protein G affinity chromatography from the concentrate of culture supernatant
• Clone No.: 3-6C2
• Subclass: IgG2a.k (k: kappa)
• Specificity: Reactive with human and mouse tenascin-C
• Preparation: PBS(-) solution (pH 7.2) containing 0.1% sodium azide as a preservative
• Concentration: 1 mg/mL
• Working Concentration: Immunohistochemistry 1 ~ 5 ug/mL (ug: micrograms

Package Insert(184kb/1p)

Application

Staining of Tumor formed after subcutaneous implantation of human ovarian tumor cells line to nude mouse

The section, which is prepared after fixing and embedding of tumor, is stained by Anti Human Tenascin-C, Rat monoclonal antibody and FITC conjugated rabbit anti-rat IgG. Then the examination under a laser-microscope was made.
Positive (green) is human and mouse tenascin-C. Red is the cell nuclei.

Fixative solution: 4% Paraformaldehyde in 0.1 mol/L phosphate buffer (pH 7.2)
Primary antibody: Anti Human Tenascin-C, Rat Monoclonal Antibody (Final concentration: 1 ~ 5 ug/mL) (ug: micrograms)
Second antibody: FITC conjugated rabbit anti-rat IgG
Mounting medium: 0.05mol/L TBS (pH 8.0), 90 % Glycerol and 10 mg/mL DABCO

References:

1. Hiraiwa, N., et al.: "Induction of tenascin in cancer cells by interactions with embryonic mesenchyme mediated by a diffusible factor", J. Cell Sci., 104, 289-96 (1993).
2. Beaulieu, J., et al.: "Expression of tenascin in the developing human small intestine", Biochem. Biophys. Res. Commun., 192, 1086-92 (1993).
3. Sasano, H., et al.:, "Tenascin expression in normal and abnormal human endometrium", Modern Pathology, 6, 323-6 (1993).
4. Settles, D. L., et al.: "Tenascin-C knockout mouse has no detectable tenascin-C protein", J. Neurosci. Res., 47, 109-17 (1997).
5. Whitlon, D. S., et al. : "Tenascin-C in the cochlea of the developing mouse", J. Comp. Neurol., 406, 361-74 (1999).

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