CSA (C3H strain specific antigen) is a variant of heat shock protein
Hspa9. CSA expresses in C3H mouse, and it does not disappear when the
cell differentiates and turns cancerous. Anti CSA, Monoclonal Antibody
is able to be used to research of regenerative medicine, cancer and so
on using C3H chimera mouse.
Antigen: Fraction containing GPI activity, which is collected
from the crude extract of C3H/HeN mouse muscles.
Purification: Purified by Protein G affinity chromatography from
nude mouse ascites.
Clone No.: 17-6C3
Specificity: Recognizes C3H strain variant of Hspa9.
Reactivity: Reactive with congenic mouse strain derived from C3H/He
and C3H, DBA1, SM/J and congenic CSA mouse from BALB/cA and C57BL/6.
Storage buffer: PBS (-) (pH 7.2) and 0.1% Sodium Azide
Concentration: 1 mg/mL
Working concentration: Immunohistochemistry ... 1 ~ 5 μg/mL
Staining of Breast cancer cells of C3H mouse
The breast cancer cells of C3H mouse and the breast cancer cells of BALB/c
mouse are co-cultured, and strained by Anti CSA, Monoclonal Antibody and
FITC conjugated goat anti-mouse IgG.
Positive (green) is the breast cancer cells of C3H. The cells of BALB/c
are not strained. Red is the cell nuclei.
Fixative solution: 95% Ethanol containing 1 % acetic acid stored in ice.
Primary antibody: Anti CSA, Monoclonal Antibody
(Final concentration: 1 μg/mL)
Second antibody: FITC conjugated goat, anti-mouse IgG
Mounting medium: 0.05 mol/L TBS (pH 8.0), 90% Glycerol and 10 mg/mL DABCO
- Kusakabe, M., et al.: J. Cell Biol., 107, 257(1988).
- Lee, G. H., et al.: Cancer Res., 51, 3257 (1991).
- Michikawa, Y., et al.: Biochem. Biophys. Res. Commun., 196, 223 (1993).
- Taniguchi, H., et al.: Transplant. Proc., 31,
- Suzuki, A., et al.: Hepatology, 32, 1230(2000).
- Suzuki, A., et al.: Transplant. Proc., 32, 2370(2000)
Wako Catalog No.
|Anti CSA, Monoclonal Antihbody
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