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for Regenerative Medical Research
Anti CSA, Monoclonal Antibody

Package Insert(192kb/2p)

CSA (C3H strain specific antigen) is a variant of heat shock protein Hspa9. CSA expresses in C3H mouse, and it does not disappear when the cell differentiates and turns cancerous. Anti CSA, Monoclonal Antibody is able to be used to research of regenerative medicine, cancer and so on using C3H chimera mouse.

Antigen: Fraction containing GPI activity, which is collected from the crude extract of C3H/HeN mouse muscles.
Purification: Purified by Protein G affinity chromatography from nude mouse ascites.
Clone No.: 17-6C3
Subclass: IgGγ1.κ
Specificity: Recognizes C3H strain variant of Hspa9.
Reactivity: Reactive with congenic mouse strain derived from C3H/He and C3H, DBA1, SM/J and congenic CSA mouse from BALB/cA and C57BL/6.
Storage buffer: PBS (-) (pH 7.2) and 0.1% Sodium Azide
Concentration: 1 mg/mL
Working concentration: Immunohistochemistry ... 1 ~ 5 μg/mL

Appilcation data

Staining of Breast cancer cells of C3H mouse

The breast cancer cells of C3H mouse and the breast cancer cells of BALB/c mouse are co-cultured, and strained by Anti CSA, Monoclonal Antibody and FITC conjugated goat anti-mouse IgG.
Positive (green) is the breast cancer cells of C3H. The cells of BALB/c are not strained. Red is the cell nuclei.
Fixative solution: 95% Ethanol containing 1 % acetic acid stored in ice.
Primary antibody: Anti CSA, Monoclonal Antibody
(Final concentration: 1 μg/mL)
Second antibody: FITC conjugated goat, anti-mouse IgG
Mounting medium: 0.05 mol/L TBS (pH 8.0), 90% Glycerol and 10 mg/mL DABCO

References:

1. Kusakabe, M., et al.: J. Cell Biol., 107, 257(1988).
2. Lee, G. H., et al.: Cancer Res., 51, 3257 (1991).
3. Michikawa, Y., et al.: Biochem. Biophys. Res. Commun., 196, 223 (1993).
4. Taniguchi, H., et al.: Transplant. Proc., 31, 454(1999).
5. Suzuki, A., et al.: Hepatology, 32, 1230(2000).
6. Suzuki, A., et al.: Transplant. Proc., 32, 2370(2000)

Product

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