Adenosine 5'-Diphosphate Disodium Salt (ADP): 2 mmol/L
D(+)-Glucose: 20 mmol/L
Nicotinamide Adenine Dinucleotide Phosphate Oxidized Form (beta-NADP+)
EDTA 2Na: 1 mmol/L
Glutathione, reduced: 20 mmol/L
Magnesium Chloride, Hexahydrate: 20 mmol/L
C: Hexokinase (1,000 units/mL)
D: Glucose 6-Phosphate Dehydrogenase (1,000 units/mL)
E: Enzyme solution
12 mg of this product is dissolved to Reagent
A to prepare the approximately 3 units/mL solution.
Preincubate at 30°C
Record the absorbance change at 340 nm after 2 ~ 5 minute incubation
at 30°C and calculate the average change in absorbance per minute (A)
according to JIS K0115.
One unit is defined as the amount of enzyme to produce 1 Êmol of NADPH per minute
at 30°C and pH 7.4.
unit/vial = [(A test - A control ) X 3.04]
/ ( 6.2 X S X 0.02 )
A test : Absorbance change per minute of Test
A control :Absorbance change per minute of Control
6.2: Millimolar extinction coefficient of NADPH
3.04: Total volume (mL)
S: Enzyme concentration in Reagent E (mg/mL)
0.02: Enzyme solution volume (mL)
Wako Catalog No.
Creatine Kinase (CK), from bovine
Keep at -20°C
Dawson, D. M. and Eppenberger, H. M.: Meth.
Enzymol., 17A, 995 (1970).