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DNA Extractor® Series available from Wako

Kit Selection by Sample Property

Sample
Whole Blood
Serum
Plasma
Tissues
Paraffin-
embedded tissue Section
Biopharma
-ceuticals
Cultured 
Cells
Hair, Nail, Bloodstain & Saliva
genomic DNA
DNA Extractor® WB Kit 
(#291-50502; 50 tests)

DNA Extractor® WB-Rapid Kit (#297-54801 (20 tests);  #293-54803 (200 tests))

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DNA Extractor® WB Kit
(#291-50502; 50 tests)
DNA Isolator PS Kit (#295-52401 (100 tests))

DNA Isolator PS-Rapid Reagent (#291-56401 (100 tests))

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DNA Extractor® WB Kit 
(#291-50502 (50 tests))
DNA Extractor® FM Kit
(#295-58501 (50 tests))
mitochondrial DNA (mtDNA)
mtDNA Extractor® WB Kit (#293-54401 (25 tests))
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mtDNA Extractor® CT Kit (#291-55301 (25 tests)
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mtDNA Extractor® CT Kit (#291-55301 (25 tests)
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free DNA
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DNA Extractor® SP Kit (#296-60501 (50 tests))
DNA Extractor® SP Kit (#296-60501 (50 tests))
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low-oxidized DNA
DNA Extractor® WB Kit 
(#291-50502; 50 tests)
 
 
DNA Extractor® TIS Kit (#296-67701 (50 tests))
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DNA Extractor® TIS Kit (#296-67701 (50 tests))
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bacterial genomic DNA from biologicals
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DNA Extractor® Kit (#295-50201 (50 tests))
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DNA Extractor® Kit (#295-50201 (50 tests))
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virus DNA
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DNA Extractor® Kit (#295-50201 (50 tests))
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DNA Extractor® Series Product List
Product
Sample
Principle
Features
Kit Contents
for residual DNA Extraction in biopharmaceuticals
DNA Extractor® Kit
Wako Cat.#295-50201 (50 tests)
Biopharmaceutical
Serum 
Sodium Iodide method

for extraction of residual DNA in biopharmaceuticals

1) Sodium Iodide Soln. 1 x 26 mL
2) Sodium N-Lauroyl
        Sarcosinate Soln. 1 x 1.2 mL
3) Washing Soln. (A) 1 x 42 mL
4) Washing Soln. (B) 2 x 40 mL
5) Glycogen Soln. 1 x 0.1 mL
for DNA Extraction from serum and plasma
DNA Extractor® SP Kit
Wako Cat. #296-60501 (50 tests)
 
 

SP comes from efficient extraction of DNA fragments from serum and plasma

Serum
Plasma
Sodium Iodide method (1) High DNA yield (abt. 100%) from small amount of serum and plasma such as 100 uL of sample.
(2) The entire extraction of DNA can be done in a single centrifuge tube.
(3) Complete removal of lipid derived from blood by using specially prepared alcohol.
1) Enzyme reaction Soln.  1 x 10 mL
2) Protein digestion Soln. 1 x 250 uL
3) Sodium iodide Soln. 1 x 15 mL
4) Alcohol Soln.  1 x 30 mL
5) Washing Soln. (A)  1 x  50 mL
6) Washing Soln. (B)  1 x 50 mL
for DNA Extraction for 8-OHdG Assay from human and animal parenchyma tissues
DNA Extractor® TIS Kit
Wako Cat. #296-67701 (50 tests)
 
 

TIS comes from tissue.

Tissue
Cultured cell
Sodium Iodide method (1) Useful for detection and determination of 8-OHdG, a marker of oxidative stress
(2) Achieve inhibition of further DNA oxidation by using an oxidation inhibitor
1) Lysis Soln.  2 x 75 mL
2) Enzyme Reaction Soln.  1 x 15 mL
3) RNase Soln.  1 x 50 uL
4) Protein Digest Soln.  1 x 750 uL
5) Oxidation Inhibitor  1 x 350 uL
6) Sodium Iodide Soln.  1 x 15 mL
7) Alcohol Soln.  1 x 30 mL
8) PEG Soln.  1 x 20 mL
for DNA Extraction from whole blood
DNA Extractor® WB Kit
Wako Cat. #291-50502 (50 tests)
 

WB comes from genomic DNA extraction from whole blood

Whole blood
Tissue
Cultured cell
Sodium Iodide method (1) High DNA recovery
(2)The entire extraction of DNA can be done in a single centrifuge tube.
1) Lysis Soln.  2 x 65 mL
2) Enzyme Reaction Soln.
         1 x 10 mL
3) Sodium Iodide Soln.  1 x 15 mL
4) Washing Soln. (A)  1 x 50 mL
5) Washing Soln. (B)  1 x 50 mL
6) Protease   1 x 10 mg
DNA Extractor® WB-Rapid Kit
Wako Cat. #297-54801 (20 tests)
Wako Cat. #293-54803 (200 tests)
Whole blood - Improved DNA Extractor® WB Kit- a smaller scale and a short time (30 minute) extraction-                      [20 tests] / [200 tests]
1) Lysis Soln. [1x10mL] / [2x50mL]
2) Enzyme Reaction Soln.
         [1 x 0.8mL] / [1 x 8mL]
3) Washing Soln.
        [1 x 20mL] / [3 x 70mL]
4) Protease Soln.
        [1 x 40uL] / [1 x 400uL]
for DNA Extraction from paraffin-embedded tissues
DNA Isolator PS Kit
Wako Cat. #295-52401 (100 tests)

PS comes from isolation from  parrafin-embedded tissue section

Paraffin-embedded tissue section
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1) Enzyme Reaction Soln.  1 x 2 mL
2) Enzyme Activator  1 x 34 mg
3) Protease  1 x 2.2 mg
4) Accelerator for DNA
      precipitation  1 x 0.5 mL
5) Dilution Soln. for DNA
     amplification  1 x 0.5 mL
DNA Isolator PS-Rapid Reagent
Wako Cat. #291-56401 (100 tests)
Paraffin-embedded tissue section Boiling method
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1) DNA Isolation Soln.  5 x 10 mL
for mitochondrial DNA Extraction from whole blood
mtDNA Extractor® WB Kit
Wako Cat. #293-54401 (25 tests)
Whole blood Sodium Iodide method In 90 min., mitochondrial DNA from whole blood can be isolated. 1) Leukocyte Isolation Soln.
         1 x 5.0 mL
2) DNA Extraction Soln. I
         1 x 26.5 mL
3) DNA Extraction Soln. II (A)
          1 x 1.3 mL
4) DNA Extraction Soln. II (B)
          1 x 1.3 mL
5) DNA Extraction Soln. III
         1 x 1.9 mL
6) Sodium Iodide Soln.  1 x 7.5 mL
7) Washing Soln.  1 x 50 mL
for mitochondrial DNA Extraction from cell culture and tissue samples
mtDNA Extractor® CT Kit
Wako Cat. #291-55301 (25 tests)
 

CT comes from mtDNA extraction from cell culture and tissue samples. 

Tissue
Cell culture
Sodium Iodide method for extraction of mitochondrial DNA from cell culture and tissue samples. 1) Buffer for Homogenate
         1 x 25.0 mL
2) DNA Extraction Soln. I
         1 x 1.3 mL
3) DNA Extraction Soln. II (A)
          1 x 1.3 mL
4) DNA Extraction Soln. II (B)
        1 x 1.3 mL
5) DNA Extraction Soln. III
         1 x 1.9 mL
6) Sodium Iodide Soln.  1 x 7.5 ml
7) Washing Soln.  1 x 50 mL
for DNA Extraction for medicolegal investigation
DNA Extractor® FM Kit
Wako Cat. #295-58501 (50 tests)

FM comes from determination method in forensic m edicine
 

hair
nail
bloodstain
saliva 
Sodium Iodide Solubilizes a variety of tough hairs as body hair completely within several ten minutes. 1) Lysis Soln.  1 x 9.5 mL
2) Enzyme-activated Reagent (EAR)
           1 x 80 mg
3) Reconstitution Soln. For EAR
          1 x 0.5 mL
4) Protease  1 x 10 mg
5) Sodium Iodide Soln.  1 x 12.5 mL
6) Washing Soln. (A)  1 x 50 mL
7) Washing Soln. (B)  1 x 50 mL
(uL: microlitters; ug: micrograms)
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