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Kit Selection by Sample Property

DNA Extractor® Series Product List

Product	Sample	Principle	Features	Kit Contents
for residual DNA Extraction in biopharmaceuticals
DNA Extractor® Kit Wako Cat.#295-50201 (50 tests)	Biopharmaceutical Serum	Sodium Iodide method	for extraction of residual DNA in biopharmaceuticals	1) Sodium Iodide Soln. 1 x 26 mL 2) Sodium N-Lauroyl         Sarcosinate Soln. 1 x 1.2 mL 3) Washing Soln. (A) 1 x 42 mL 4) Washing Soln. (B) 2 x 40 mL 5) Glycogen Soln. 1 x 0.1 mL
for DNA Extraction from serum and plasma
DNA Extractor® SP Kit Wako Cat. #296-60501 (50 tests)     SP comes from efficient extraction of DNA fragments from serum and plasma	Serum Plasma	Sodium Iodide method	(1) High DNA yield (abt. 100%) from small amount of serum and plasma such as 100 uL of sample. (2) The entire extraction of DNA can be done in a single centrifuge tube. (3) Complete removal of lipid derived from blood by using specially prepared alcohol.	1) Enzyme reaction Soln.  1 x 10 mL 2) Protein digestion Soln. 1 x 250 uL 3) Sodium iodide Soln. 1 x 15 mL 4) Alcohol Soln.  1 x 30 mL 5) Washing Soln. (A)  1 x  50 mL 6) Washing Soln. (B)  1 x 50 mL
for DNA Extraction for 8-OHdG Assay from human and animal parenchyma tissues
DNA Extractor® TIS Kit Wako Cat. #296-67701 (50 tests)     TIS comes from tissue.	Tissue Cultured cell	Sodium Iodide method	(1) Useful for detection and determination of 8-OHdG, a marker of oxidative stress (2) Achieve inhibition of further DNA oxidation by using an oxidation inhibitor	1) Lysis Soln.  2 x 75 mL 2) Enzyme Reaction Soln.  1 x 15 mL 3) RNase Soln.  1 x 50 uL 4) Protein Digest Soln.  1 x 750 uL 5) Oxidation Inhibitor  1 x 350 uL 6) Sodium Iodide Soln.  1 x 15 mL 7) Alcohol Soln.  1 x 30 mL 8) PEG Soln.  1 x 20 mL
for DNA Extraction from whole blood
DNA Extractor® WB Kit Wako Cat. #295-50502 (50 tests)   WB comes from genomic DNA extraction from whole blood	Whole blood Tissue Cultured cell	Sodium Iodide method	(1) High DNA recovery (2)The entire extraction of DNA can be done in a single centrifuge tube.	1) Lysis Soln.  2 x 65 mL 2) Enzyme Reaction Soln.          1 x 10 mL 3) Sodium Iodide Soln.  1 x 15 mL 4) Washing Soln. (A)  1 x 50 mL 5) Washing Soln. (B)  1 x 50 mL 6) Protease   1 x 10 mg
DNA Extractor® WB-Rapid Kit Wako Cat. #297-54801 (20 tests) Wako Cat. #293-54803 (200 tests)	Whole blood	-	Improved DNA Extractor® WB Kit- a smaller scale and a short time (30 minute) extraction-	[20 tests] / [200 tests] 1) Lysis Soln. [1x10mL] / [2x50mL] 2) Enzyme Reaction Soln.          [1 x 0.8mL] / [1 x 8mL] 3) Washing Soln.         [1 x 20mL] / [3 x 70mL] 4) Protease Soln.         [1 x 40uL] / [1 x 400uL]
for DNA Extraction from paraffin-embedded tissues
DNA Isolator PS Kit Wako Cat. #295-52401 (100 tests) PS comes from isolation from  parrafin-embedded tissue section	Paraffin-embedded tissue section	-	-	1) Enzyme Reaction Soln.  1 x 2 mL 2) Enzyme Activator  1 x 34 mg 3) Protease  1 x 2.2 mg 4) Accelerator for DNA       precipitation  1 x 0.5 mL 5) Dilution Soln. for DNA      amplification  1 x 0.5 mL
DNA Isolator PS-Rapid Reagent Wako Cat. #291-56401 (100 tests)	Paraffin-embedded tissue section	Boiling method	-	1) DNA Isolation Soln.  5 x 10 mL
for mitochondrial DNA Extraction from whole blood
mtDNA Extractor® WB Kit Wako Cat. #293-54401 (25 tests)	Whole blood	Sodium Iodide method	In 90 min., mitochondrial DNA from whole blood can be isolated.	1) Leukocyte Isolation Soln.          1 x 5.0 mL 2) DNA Extraction Soln. I          1 x 26.5 mL 3) DNA Extraction Soln. II (A)           1 x 1.3 mL 4) DNA Extraction Soln. II (B)           1 x 1.3 mL 5) DNA Extraction Soln. III          1 x 1.9 mL 6) Sodium Iodide Soln.  1 x 7.5 mL 7) Washing Soln.  1 x 50 mL
for mitochondrial DNA Extraction from cell culture and tissue samples
mtDNA Extractor® CT Kit Wako Cat. #291-55301 (25 tests)   CT comes from mtDNA extraction from cell culture and tissue samples.	Tissue Cell culture	Sodium Iodide method	for extraction of mitochondrial DNA from cell culture and tissue samples.	1) Buffer for Homogenate          1 x 25.0 mL 2) DNA Extraction Soln. I          1 x 1.3 mL 3) DNA Extraction Soln. II (A)           1 x 1.3 mL 4) DNA Extraction Soln. II (B)         1 x 1.3 mL 5) DNA Extraction Soln. III          1 x 1.9 mL 6) Sodium Iodide Soln.  1 x 7.5 ml 7) Washing Soln.  1 x 50 mL
for DNA Extraction for medicolegal investigation
DNA Extractor® FM Kit Wako Cat. #295-58501 (50 tests) FM comes from determination method in forensic m edicine	hair nail bloodstain saliva	Sodium Iodide	Solubilizes a variety of tough hairs as body hair completely within several ten minutes.	1) Lysis Soln.  1 x 9.5 mL 2) Enzyme-activated Reagent (EAR)            1 x 80 mg 3) Reconstitution Soln. For EAR           1 x 0.5 mL 4) Protease  1 x 10 mg 5) Sodium Iodide Soln.  1 x 12.5 mL 6) Washing Soln. (A)  1 x 50 mL 7) Washing Soln. (B)  1 x 50 mL