Anti Modified Histone H3 Antibody

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Histone modification involves epigenetic regulations of gene expression and various regulation of chromosome function. Highly specific modified histone antibodies have been eagerly anticipated for Chromatin immunoprecipitation (ChIP) assays to detect modified histones on particular genome regions. Wako has launched such modified histone antibodies manufactured by MAB Institute Inc. (Japan). They have solved the problem of high lot-to-lot variation of the rabbit polyclonal antibodies.

・High specificity to modified histone
・Each specificity is confirmed with each modified peptide
・Little lot-to-lot variation
・Applicable to ChIP Assay

	Immunoprecipitated gene regions with control IgG and Anti dimethyl Histone H3(Lys4) (Wako Cat. #308-34809), are amplified using optimized primers, respectively.

Preparation:
Concentration:
Working Dilution:

PBS solution containing 0.05% Sodium Azide as preservative
1 mg/mL
[ChIP] 1 ~ 5 μg/5 μL
[Sepharose Immunostaining] 0.5 ~ 1 μg/mL,
[Immunoblotting] 0.5 ~ 1 μg/mL

Description	residue	modification	Clone No.	Pkg. Size	Wako Cat. No.
Anti-Histone H3, mouse mAb	-	unmodified	MABI0301	100μL	308-34789
				20 μL	302-34782
Anti-monomethyl Histone H3(Lys4), mouse mAb	K4	monomethyl	MABI0302	100 μL	305-34799
				20 μL	309-34792
Anti-dimethyl Histone H3 (Lys4), mouse mAb		dimethyl	MABI0303	100 μL	308-34809
				20 μL	302-34802
Anti-trimethyl Histone H3(Lys4), mouse mAb		trimethyl	MABI0304	100 μL	305-34819
				20 μL	309-34812
Anti-acethyl Histone H3 (Lys9), mouse mmAb	K9	acetyl	MABI0305	100 μL	309-32379
				20 μL	303-32372
Anti-monomethyl Histone H3(Lys9), mouse mAb		monomethyl	MABI0306	100 μL	302-34829
				20 μL	306-34822
Anti-dimethyl Histone H3 (Lys9), mouse mAb		dimethyl	MABI0307	100 μL	302-32369
				20 μL	306-32362
Anti-trimethyl Histone H3(Lys9), mouse mAb		trimethyl	MABI0308	100 μL	309-34839
				20 μL	303-34832
Anti-acetyl Histone H3(Lys9/27), mouse mAb	K9/K27	acetyl	MABI0310	100 μL	303-34859
				20 μL	307-34852
Anti-acetyl Histone H3(Lys27), mouse mAb	K27	acetyl	MABI0309	100 μL	306-34849
				20 μL	300-34842
Anti-monomethyl Histone H3(Lys27), mouse mAb		monomethyl	MABI0321	100 μL	305-95239
				20 μL	309-95232
Anti-dimethyl Histone H3(Lys27), mouse mAb		dimethyl	MABI0322	100 μL	302-95249
				20 μL	306-95242
Anti-trimethyl Histone H3(Lys27), mouse mAb		trimethyl	MABI0323	100 μL	309-95259
				20 μL	303-95252
Anti-phospho Histone H3(Ser10), mouse mAb	S10	phospho	MABI0312	100 μL	303-35199
				20 μL	307-35192
Anti-monomethyl Histone H3(Lys36), mouse mAb	K36	monomethyl	MABI0331	100 μL	306-95269
				20 μL	300-95262
Anti-dimethyl Histone H3 (Lys36), mouse mAb		dimethyl	MABI0332	100 μL	303-95279
				20 μL	307-95272
Anti-trimethyl Histone H3(Lys36), mouse mAb		trimethyl	MABI0333	100 μL	300-95289
				20 μL	304-95282
Anti-phospho Histone H2B(Ser14), mouse mAb	S14	phospho	MABI0251	100 μL	308-95229
				20 μL	302-95222

Reference:

Kimura, H., Hayashi-Takanaka, Y. Goto, Y., Takizawa, N. and Nozaki, N.,
"The Organization of Histone H3Modifications as Revealed by a Panel of Specific Monoclonal Antibodies",
Cell Struct. Funct., 33(1), 61-73 (2008)