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for Stevioside Determination
Highly-Purified Stevia Standards

Leaflet(56KB/1p)

Stevia rebaudiana (Stevia) is a plant native to South America. The leaf of the Stevia plant contains sweet components, called steviol glycosides, including Stevioside, Rebaudioside A and others. The Stevia extract has been listed as a food additive on the 8th edition of Japan's Specifications and Standards for Food Additives and has been used as a sweetener around the world. Wako can provide the highly purified standards and they can be used for accurate determination of the steviol glycosides.

Stevia rebaudiana Bertoni			HPLC chart of Rebaudioside A Standard (Wako Cat. #189-02581)

Specification

Test	Isosteviol Std.	Rebaudioside A Std.	Rebaudioside B Std.	Rubusoside Std.	Stevioside Std.
Appearance	White, crystalline powder	White, crystalline powder	White~, crystalline powder ~ powder	White~, crystalline powder ~ powder	White, powder
Solubility	Pass the test (in 1,4-Dioxane)	Pass the test (in Water-MeOH)	Pass the test (in MeOH)	Pass the test (in MeOH)	Pass the test (in water)
Melting Point	229 ~ 232 degrees C	-	-	-	-
Specific Rotation (@20ºC)	-80.5 ~ -77.5º (in EtOH)	-20 ~ -24º (in MeOH)	Report measured value (in MeOH)	Report measured value (in MeOH)	Report measured value (in water)
Loss on Drying (for 2 hr.)	max. 5.0 % (@105 ºC)	max. 5.0 % (@110ºC)	max. 5.0 % (@105ºC)	max. 5.0 % (@105ºC)	max. 5.0 % (@105ºC)
TLC test	Pass the test	Pass the test	Pass the test	Pass the test	Pass the test
Assay	min. 99.0 % (HPLC, after drying)	min. 99.0 % (HPLC, after drying)	min. 99.0 % (HPLC, after drying)	min. 98.0 % (HPLC, after drying)	min. 99.0 % (HPLC, after drying)
Assay	min. 99.0 % (Volumetric analysis)	-	-	-	-

Stevia Reference Standards for Steviol Glycoside Determination

Description	Wako Cat. No.	Package Size
Isosteviol Standard	098-05681	1 g
Rebaudioside A Standard	189-02581	100 mg
Rebaudioside A Standard	185-02583	1 g
Rebaudioside B Standard	188-02551	25 mg
Rebaudioside B Standard	184-02553	100 mg
Rubusoside Standard	187-02521	25 mg
Rubusoside Standard	183-02523	100 mg
Stevioside Standard	193-15351	1 g

Note

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