Wako Japan Online Catalog Wako USA Wako GmbH Contact Us

Life Science

Life Science

Print (175Kb)

Histone modification involves epigenetic regulations of gene expression and various regulation of chromosome function. Highly specific modified histone antibodies have been eagerly anticipated for Chromatin immunoprecipitation (ChIP) assays to detect modified histones on particular genome regions. Wako has launched such modified histone antibodies manufactured by MAB Institute Inc. (Japan). They have solved the problem of high lot-to-lot variation of the rabbit polyclonal antibodies.
EHigh specificity to modified histone
EEach specificity is confirmed with each modified peptide
ELittle lot-to-lot variation
EApplicable to ChIP Assay
Immunoprecipitated gene regions with control IgG and
Anti dimethyl Histone H3(Lys4) (Wako Cat. #308-34809),
are amplified using optimized primers, respectively.
				
Preparation:
Concentration:
Working Dilution:
PBS solution containing 0.05% Sodium Azide as preservative
1 mg/mL
[ChIP] 1 ~ 5 g/5 L
[Sepharose Immunostaining] 0.5 ~ 1 g/mL,
[Immunoblotting] 0.5 ~ 1 g/mL
Description residue modification Clone No. Pkg. Size Wako Cat. No.
Anti-Histone H3, mouse mAb - unmodified MABI0301 100 L 308-34789
20 L 302-34782
Anti-monomethyl Histone H3(Lys4), mouse mAb K4 monomethyl MABI0302 100 L 305-34799
20 L 309-34792
Anti-dimethyl Histone H3 (Lys4), mouse mAb dimethyl MABI0303 100 L 308-34809
20 L 302-34802
Anti-trimethyl Histone H3(Lys4), mouse mAb trimethyl MABI0304 100 L 305-34819
20 L 309-34812
Anti-acethyl Histone H3 (Lys9), mouse mmAb K9 acetyl MABI0305 100 L 309-32379
20 L 303-32372
Anti-monomethyl Histone H3(Lys9), mouse mAb monomethyl MABI0306 100 L 302-34829
20 L 306-34822
Anti-dimethyl Histone H3 (Lys9), mouse mAb dimethyl MABI0307 100 L 302-32369
20 L 306-32362
Anti-trimethyl Histone H3(Lys9), mouse mAb trimethyl MABI0308 100 L 309-34839
20 L 303-34832
Anti-acetyl Histone H3(Lys9/27), mouse mAb K9/K27 acetyl MABI0310 100 L 303-34859
20 L 307-34852
Anti-acetyl Histone H3(Lys27), mouse mAb K27 acetyl MABI0309 100 L 306-34849
20 L 300-34842
Anti-phospho Histone H3(Ser10), mouse mAb S10 phospho MABI0312 100 L 303-35199
20 L 307-35192
Reference: Kimura, H., Hayashi-Takanaka, Y. Goto, Y., Takizawa, N. and Nozaki, N.,
"The Organization of Histone H3 Modifications as Revealed by a Panel of Specific Monoclonal Antibodies",
Cell Struct. Funct., 33(1), 61-73 (2008)