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Histone modification involves epigenetic regulations of gene expression and various regulation of chromosome function. Highly specific modified histone antibodies have been eagerly anticipated for Chromatin immunoprecipitation (ChIP) assays to detect modified histones on particular genome regions.
Wako has launched such modified histone antibodies manufactured by MAB Institute Inc. (Japan). They have solved the problem of high lot-to-lot variation of the rabbit polyclonal antibodies.
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・High specificity to modified histone
・Each specificity is confirmed with each modified peptide
・Little lot-to-lot variation
・Applicable to ChIP Assay
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Immunoprecipitated gene regions with control IgG and
Anti dimethyl Histone H3(Lys4) (Wako Cat. #308-34809),
are amplified using optimized primers, respectively.
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Preparation:
Concentration:
Working Dilution:
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PBS solution containing 0.05% Sodium Azide as preservative
1 mg/mL
[ChIP] 1 ~ 5 μg/5 μL
[Sepharose Immunostaining] 0.5 ~ 1 μg/mL,
[Immunoblotting] 0.5 ~ 1 μg/mL
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| Description |
residue |
modification |
Clone No. |
Pkg. Size |
Wako Cat. No. |
| Anti-Histone H3, mouse mAb |
- |
unmodified |
MABI0301 |
100 μL |
308-34789 |
| 20 μL |
302-34782 |
| Anti-monomethyl
Histone H3(Lys4), mouse mAb |
K4 |
monomethyl |
MABI0302 |
100 μL |
305-34799 |
| 20 μL |
309-34792 |
| Anti-dimethyl Histone H3 (Lys4),
mouse mAb |
dimethyl |
MABI0303 |
100 μL |
308-34809 |
| 20 μL |
302-34802 |
| Anti-trimethyl
Histone H3(Lys4), mouse mAb |
trimethyl |
MABI0304 |
100 μL |
305-34819 |
| 20 μL |
309-34812 |
| Anti-acethyl Histone H3 (Lys9), mouse
mmAb |
K9 |
acetyl |
MABI0305 |
100 μL |
309-32379 |
| 20 μL |
303-32372 |
| Anti-monomethyl
Histone H3(Lys9), mouse mAb |
monomethyl |
MABI0306 |
100 μL |
302-34829 |
| 20 μL |
306-34822 |
| Anti-dimethyl Histone H3 (Lys9),
mouse mAb |
dimethyl |
MABI0307 |
100 μL |
302-32369 |
| 20 μL |
306-32362 |
| Anti-trimethyl
Histone H3(Lys9), mouse mAb |
trimethyl |
MABI0308 |
100 μL |
309-34839 |
| 20 μL |
303-34832 |
| Anti-acetyl Histone H3(Lys9/27),
mouse mAb |
K9/K27 |
acetyl |
MABI0310 |
100 μL |
303-34859 |
| 20 μL |
307-34852 |
| Anti-acetyl Histone
H3(Lys27), mouse mAb |
K27 |
acetyl |
MABI0309 |
100 μL |
306-34849 |
| 20 μL |
300-34842 |
| Anti-phospho Histone H3(Ser10), mouse
mAb |
S10 |
phospho |
MABI0312 |
100 μL |
303-35199 |
| 20 μL |
307-35192 |
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| Reference: |
Kimura, H., Hayashi-Takanaka, Y. Goto, Y., Takizawa, N. and Nozaki, N.,
"The Organization of Histone H3 Modifications as Revealed by a Panel of Specific Monoclonal Antibodies",
Cell Struct. Funct., 33(1), 61-73 (2008)
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Wako Japan
