1. for the Japanese Pharmacopoeia (JP) Crude Drug Test

2. for Crude Drug Test

Alphabetical index

print (3.5 MB/44P)   

Product Name Wako Cat. No. Chemical Structural Formula

Achyranthes Root

017-22731

Unidentified

Crude Drug Pkg. Size

Achyranthes root

5 g

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Aconitum Diester Alkaloids Standard

018-23741

018-23741
Crude Drug Pkg. Size

Aconiti Rhizoma

0.35 mg

Grade Storage

for the JP Crude Drugs Test

(for Purity Determination)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Aconitum Monoester Alkaloids Standard

014-23721

014-23721
Crude Drug Pkg. Size

Aconiti Rhizoma

0.25 mg

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Albiflorin, 98.0+ % (HPLC)

016-22201

016-22201

C23H28O11 = 480.46

CAS No. 39011-90-0

Crude Drug Pkg. Size

Paeoniae Radix

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Alisol A, 97.0+ % (HPLC)

018-20681

018-20681

C30H50O5 = 490.72

CAS No. 19885-10-0

Crude Drug Pkg. Size

Alismatis Rhizoma

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at -20°C.

Product Name Wako Cat. No. Chemical Structural Formula

Amygdalin

017-23571

017-23571

C20H27NO11 = 457.43

CAS No. 29883-15-6

Crude Drug Pkg. Size

·Armeniacae Semen

·Persicae Semen

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Asarinin, 98.0+ % (HPLC)

012-24121

012-24121

C20H18O6 = 354.35

CAS No. 133-04-0

Crude Drug Pkg. Size

asiasarum root

10 mg

Grade Storage

for the JP Crude Drugs Test

(for Thin-Layer Chromatography)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-Asarone, 98.0+ % (HPLC)

013-22571

013-22571

C12H16O3 = 208.25

CAS No. 2883-98-9

Crude Drug Pkg. Size

Perilla Herb

10 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Astragaloside IV, 98.0+ % (HPLC)

015-20691

015-20691

C41H68O14 = 784.97

CAS No. 84687-43-4

Crude Drug Pkg. Size

Astragali Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Atractylenolide III

018-24461

018-24461

C15H20O3 = 248.32

CAS No. 73030-71-4

Crude Drug Pkg. Size

Atractylodes rhizome

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Atractylodin TS (5.0 mg/L in methanol)

015-24471

015-24471

C13H10O = 182.22

CAS No. 55290-63-6

Crude Drug Pkg. Size

Atractylodes rhizome

1 mL x 5A

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Atropine Sulfate Hydrate, 99.0+ % (HPLC)

011-23731

011-23731

(C17H23NO3)2·H2SO4·H2O = 694.83

CAS No. 5908-99-6

Crude Drug Pkg. Size

·Belladonnae Radix

·Ephedra Herb

·Scopolia Rhizome

50 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Arbutin

019-23771

019-23771

C12H16O7 = 272.25

Crude Drug Pkg. Size

Uvae Ursi Folium

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Artemisia Argyi

018-24221

Unidentified

Crude Drug Pkg. Size

Artemisiae Folium

10g

Grade Storage

for the JP Crude Drugs Test

(for Purity Determination)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Baicalin, 98.0+ % (HPLC)

024-15691

024-15691

C21H18O11·H2O = 464.38

CAS No. 21967-41-9

Crude Drug Pkg. Size

Scutellariae Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Barbaloin

022-16971

022-16971

C21H22O9 = 418.39

CAS No. 1415-73-2

Crude Drug Pkg. Size

Aloe

50 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Benz [a] anthracene

023-16761

023-16761

C18H12 = 228.29

CAS No. 56-55-3

Crude Drug Pkg. Size

Wood-tar creosote

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Benzo [a] pyrene

026-16751

026-16751

C20H12 = 252.31

CAS No. 50-32-8

Crude Drug Pkg. Size

Wood-tar creosote

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Benzoylmesaconine Hydrochloride

022-16611

022-16611

C31H43NO10·HCl = 626.13

Crude Drug Pkg. Size

Aconiti Rhizoma

5 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Berberine Chloride Hydrate, 95.0 - 102.0 %
(calculated on the dehydrous basis)

025-17181

025-17181

C20H18ClNO4 = 371.81

CAS No. 141433-60-5

Crude Drug Pkg. Size

·Corydalis Tuber

·Phellodendri Cortex

·Coptidis Rhizoma

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Bergenin, 99.0+ % (HPLC)

027-16421

027-16421

C14H16O9 = 328.27

CAS No. 477-90-7

Crude Drug Pkg. Size

Mallotus japonicus (Thunb.) Muell. Arg.

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Bisdemethoxycurcumin

020-16411

020-16411

C19H16O4 = 308.33

CAS No. 33171-05-0

Crude Drug Pkg. Size

Curcuma longa L.

10 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Bufalin

029-16981

029-16981

C24H34O4 = 386.52

CAS No. 465-21-4

Crude Drug Pkg. Size

Bufo bufo gargarizans Cantor

20 mg

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-Capsaicin

034-21121

034-21121

C18H27NO3 = 305.41

CAS No. 404-86-4

Crude Drug Pkg. Size

Capsici Fructus

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Chikusetsusaponin IV, 98.0+ % (HPLC)

035-23091

035-23091

C47H74O18 = 927.08

CAS No. 7518-22-1

Crude Drug Pkg. Size

Panax Rhizome

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-Chlorogenic Acid, 98.0+ % (HPLC)

039-20331

This product may be deteriorated phototermally,
so please use it in a cool dark place.
039-20331

C16H18O9 = 354.31

CAS No. 327-97-9

Crude Drug Pkg. Size

Lonicerae Folium Cum Caulis

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-Cinnamaldehyde, 98.0+ % (HPLC)

034-21981

This product may be deteriorated phototermally,
so please use it in a cool dark place.
034-21981

C9H8O = 132.16

CAS No. 14371-10-9

Crude Drug Pkg. Size

Cinnamomi Cortex

1 mL x 5

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-Cinnamic Acid

037-22211

037-22211

C9H8O2 = 148.16

CAS No. 140-10-3

Crude Drug Pkg. Size

Cinnamomi Cortex

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Cinobufagin, 98.0+ % (JP method)

037-21971

037-21971

C26H34O6 = 442.54

CAS No. 470-37-1

Crude Drug Pkg. Size

Bufo bufo gargarizans Cantor

20 mg

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Coptisine Chloride, 98.0+ % (HPLC)

038-22001

038-22001

C19H14NO4Cl = 355.77

CAS No. 6020-18-4

Crude Drug Pkg. Size

Coptidis Rhizoma

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Curcumin

031-21991

031-21991

C21H20O6 = 368.38

CAS No. 458-37-7

Crude Drug Pkg. Size

Curcuma longa L.

10 mg

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Cycloartenyl Ferulate, 98.0+ % (HPLC)

035-22011

035-22011

C40H58O4 = 602.89

CAS No. 21238-33-5

Crude Drug Pkg. Size

Oryzae Semen

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at -20°C.

Product Name Wako Cat. No. Chemical Structural Formula

Dehydrocorydaline Nitrate

045-32941

045-32941

C22H24N2O7 = 428.44

CAS No. 13005-09-9

Crude Drug Pkg. Size

Corydalis Tuber

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Demethoxycurcumin

049-31001

049-31001

C20H18O5 = 338.35

CAS No. 22608-11-3

Crude Drug Pkg. Size

Curcuma longa L.

10 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Eleutheroside B

051-07551

051-07551

C17H24O9 = 372.37

CAS No. 118-34-3

Crude Drug Pkg. Size

Siberian Ginseng

20 mg

Grade Storage

for the JP Crude Drugs Test (for LC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-Ferulic Acid

067-06091

067-06091

C10H10O4 = 194.18

CAS No. 537-98-4

Crude Drug Pkg. Size

·Angelicae Radix

·Cimicifugae Rhizoma

20 mg

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Fructose, 99.0+ % (HPLC)

060-06081

060-06081

C6H12O6 = 180.16

CAS No. 57-48-7

Crude Drug Pkg. Size

Rehmanniae Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Geniposide, 95.0+ % (qNMR)

073-05891

073-05891

C17H24O10 = 388.37

CAS No. 24512-63-8

Crude Drug Pkg. Size

Gardeniae Fructus

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Gentiopicroside, 98.0+ % (HPLC)

073-05031

073-05031

C16H20O9 = 356.32

CAS No. 20831-76-9

Crude Drug Pkg. Size

·Gentianae Radix

·Gentianae Scabrae Radix

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

[6]-Gingerol

076-05901

076-05901

C17H26O4 = 294.39

Crude Drug Pkg. Size

Zingiberis Rhizoma

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Ginsenoside Rc, 98.0+ % (HPLC)

075-05231

075-05231

C53H90O22 = 1079.27

CAS No. 11021-14-0

Crude Drug Pkg. Size

·Ginseng Radix Rubra

·Panacis Ginseng Radix

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Ginsenoside Re, 99.0+ % (HPLC)

072-05241

072-05241

C48H82O18 = 947.15

CAS No. 52286-59-6

Crude Drug Pkg. Size

·Ginseng Radix Rubra

·Panacis Ginseng Radix

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Ginsenoside-Rg1, 99.0+ % (HPLC)

076-05021

076-05021

C42H72O14 = 801.01

CAS No. 22427-39-0

Crude Drug Pkg. Size

·Ginseng Radix Rubra

·Panacis Ginseng Radix

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

4'-O-Glucosyl-5-O-methylvisamminol,
98.0+ % (HPLC)

076-05761

076-05761

C22H28O10 = 452.45

CAS No. 84272-85-5

Crude Drug Pkg. Size

Glehniae Radix cum Rhizoma

5 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Glycyrrhizic Acid, 99.0+ % (HPLC)

070-05161

070-05161

C42H62O16 = 822.93

CAS No. 1405-86-3

Crude Drug Pkg. Size

Glycyrrhizae Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Guaiacol

073-05771

073-05771

C7H8O2 = 124.14

CAS No. 90-05-1

Crude Drug Pkg. Size

Wood-tar creosote

500 mg

Grade Storage

for the JP General Tests (for Determination)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Hesperidin

085-09311

085-09311

C28H34O15 = 610.56

CAS No. 520-26-3

Crude Drug Pkg. Size

Aurantii Nobilis Pericarpium

10 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Hirsutine

085-09291

085-09291

C22H28N2O3 = 368.47

CAS No. 7729-23-9

Crude Drug Pkg. Size

Uncariae Uncis Cum Ramulus

5 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Honokiol, 99.0+ % (HPLC)

083-08511

083-08511

C18H18O2 = 266.33

CAS No. 35354-74-6

Crude Drug Pkg. Size

Magnoliae Cortex

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

10-Hydroxy-2-(E)-decenoic Acid

081-09271

081-09271

C10H18O3 = 186.25

CAS No. 14113-05-4

Crude Drug Pkg. Size

Royal jelly

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

3-(3-Hydroxy-4-methoxyphenyl)-2-(E)-
propenoic Acid, 98.0+ % (HPLC)

085-08691

085-08691

C10H10O4 = 194.18

CAS No. 537-73-5

Crude Drug Pkg. Size

Cimicifugae Rhizoma

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Hyodeoxycholic Acid, 98.0+ % (HPLC)

089-09211

089-09211

C24H40O4 = 392.57

CAS No. 83-49-8

Crude Drug Pkg. Size

·Powdered Swine Bile

·Fel Ursi

50 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Hyperoside

087-08651

087-08651

C21H20O12 = 464.38

CAS No. 482-36-0

Crude Drug Pkg. Size

Crataegi Fructus

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Icariin, 99.0+ % (HPLC)

094-05281

094-05281

C33H40O15 = 676.66

CAS No. 489-32-7

Crude Drug Pkg. Size

Epimedii Herba

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Limonin, 98.0+ % (HPLC)

123-06101

123-06101

C26H30O8 = 470.51

CAS No. 1180-71-8

Crude Drug Pkg. Size

Phellodendri Cortex

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Liquiritin, 98.0+ % (HPLC)

129-05341

129-05341

C21H22O9 = 418.39

CAS No. 551-15-5 (anhy)

Crude Drug Pkg. Size

Glycyrrhizae Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Loganin

128-05931

128-05931

C17H26O10 = 390.38

CAS No. 18524-94-2

Crude Drug Pkg. Size

·Corni Fructus

·Lonicerae Folium Cum Caulis

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Luteolin, 98.0+ % (HPLC)

125-05441

125-05441

C15H10O6 = 286.24

CAS No. 491-70-3

Crude Drug Pkg. Size

Chrysanthemi Flos

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Magnolol, 98.0+ % (qNMR)

130-16781

130-16781

C18H18O2 = 266.33

CAS No. 528-43-8

Crude Drug Pkg. Size

Magnoliae Cortex

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Manninotriose, 98.0+ % (HPLC)

135-17711

135-17711

C18H32O16 = 504.44

CAS No. 13382-86-0

Crude Drug Pkg. Size

Rehmanniae Radix

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

l-Menthol, 99.0+ % (JP method)

132-16562

132-16562

C10H20O = 156.27

CAS No. 2216-51-5

Crude Drug Pkg. Size

Menthae Herba

25 g

Grade Storage

for the JP General Tests(for Determination)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(E)-2-Methoxycinnamaldehyde,

98.0+ % (HPLC)

134-16181

134-16181

C10H10O2 = 162.19

CAS No. 1504-74-1

Crude Drug Pkg. Size

Cinnamomi Cortex

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Myristicin, 98.0+ % (HPLC)

137-16291

137-16291

C11H12O3 = 192.21

CAS No. 607-91-0

Crude Drug Pkg. Size

Myristica fragrans

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at -20°C.

Product Name Wako Cat. No. Chemical Structural Formula

Naringin, 99.0+ % (HPLC)

149-07901

149-07901

C27H32O14 = 616.57

CAS No. 10236-47-2

Crude Drug Pkg. Size

·Rhei Rhizoma

·Aurantii Nobilis Pericarpium

·Aurantii Pericarpium

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Nodakenin, 98.0+ % (HPLC)

148-08571

148-08571

C20H24O9 = 408.40

CAS No. 495-31-8

Crude Drug Pkg. Size

Peucedani Radix

5 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Osthole, 99.0+ % (HPLC)

151-02641

151-02641

C15H16O3 = 244.29

CAS No. 484-12-8

Crude Drug Pkg. Size

Cnidii Monnieri Fructus

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Paeoniflorin, 98.0+ % (HPLC)

164-21731

164-21731

C23H28O11 = 480.46

CAS No. 23180-57-6

Crude Drug Pkg. Size

Paeoniae Radix

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Paeonol, 98.0+ % (qNMR)

164-24891

164-24891

C9H10O3 = 166.17

CAS No. 552-41-0

Crude Drug Pkg. Size

Paeoniae Moutan Cortex

10 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Palmatine Chloride, 99.0+ % (HPLC)

165-22481

165-22481

C21H22ClNO4 = 387.86

CAS No. 10605-02-4

Crude Drug Pkg. Size

Phellodendri Cortex

20 mg

Grade Storage

for the JP General Tests

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Perillaldehyde

161-24161

161-24161

C10H14O = 150.22

CAS No. 2111-75-3

Crude Drug Pkg. Size

Perillae Herba

30 mg x 5A

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Plantago Seed

168-24171

Unidentified

Crude Drug Pkg. Size

Plantaginis Semen

10g

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Powdered Swine Bile

168-24311

Unidentified

Crude Drug Pkg. Size

Fel Ursi

1g

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

(±)-Praeruptorin A, 98.0+ % (HPLC)

165-25281

165-25281

C21H22O7 = 386.40

CAS No. 73069-25-7

Crude Drug Pkg. Size

Peucedani Radix

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Puerarin, 98.0+ % (HPLC)

165-22001

165-22001

C21H20O9 = 416.38

CAS No. 3681-99-0

Crude Drug Pkg. Size

Puerariae Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Resibufogenin, 98.0+ % (JP method)

185-02681

185-02681

C24H32O4 = 384.51

CAS No. 465-39-4

Crude Drug Pkg. Size

Bufo bufo gargarizans Cantor

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Rhein, 98.0+ % (HPLC)

180-02251

180-02251

C15H8O6 = 284.22

CAS No. 478-43-3

Crude Drug Pkg. Size

Rhei Rhizoma

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Rhynchophylline

188-02671

188-02671

C22H28N2O4 = 384.47

CAS No. 76-66-4

Crude Drug Pkg. Size

Uncariae Uncis Cum Ramulus

10 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Rosmarinic Acid

181-02661

181-02661

C18H16O8 = 360.31

CAS No. 20283-92-5

Crude Drug Pkg. Size

Perillae Herba

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Saikosaponin a

192-16281

192-16281

C42H68O13 = 780.98

CAS No. 20736-09-8

Crude Drug Pkg. Size

Bupleuri Radix

25 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Saikosaponin b2

199-16171

199-16171

C42H68O13 = 780.98

CAS No. 58316-41-9

Crude Drug Pkg. Size

Bupleuri Radix

20 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Saikosaponin d

199-16311

199-16311

C42H68O13 = 780.98

CAS No. 20874-52-6

Crude Drug Pkg. Size

Bupleuri Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for Assay)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Schizandrin, 99.0+ % (HPLC)

197-13551

197-13551

C24H32O7 = 432.51

CAS No. 7432-28-2

Crude Drug Pkg. Size

Schisandrae Fructus

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at RT.

Product Name Wako Cat. No. Chemical Structural Formula

Scopoletin, 98.0+ % (HPLC)

191-16751

191-16751

C10H8O4 = 192.17

CAS No. 92-61-5

Crude Drug Pkg. Size

Artemisiae Folium

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Sesamin, 98.0+ % (HPLC)

197-16231

197-16231

C20H18O6 = 354.35

CAS No. 607-80-7

Crude Drug Pkg. Size

Sesami Semen

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at -20°C.

Product Name Wako Cat. No. Chemical Structural Formula

[6]-Shogaol

192-16161

192-16161

C17H24O3 = 276.37

CAS No. 555-66-8

Crude Drug Pkg. Size

Zingiberis Siccatum Rhizoma

10 mg

Grade Storage

for the JP Crude Drugs Test

(for Assay and TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Sodium Glycocholate, 98.0+ % (HPLC)

197-15491

197-15491

C26H42NNaO6 = 487.60

CAS No. 863-57-0

Crude Drug Pkg. Size

Fel Ursi

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Sodium Tauroursodeoxycholate,

98.0+ % (HPLC)

190-15501

190-15501

C26H44NNaO6S = 521.69

CAS No. 35807-85-3

Crude Drug Pkg. Size

Fel Ursi

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Stachyose, 99.0+ % (HPLC)

195-16911

195-16911

C24H42O21 = 666.58

CAS No. 470-55-3

Crude Drug Pkg. Size

Rehmanniae Radix

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Swertiamarin, 99.0+ % (HPLC)

191-13951

191-13951

C16H22O10 = 374.34

CAS No. 17388-39-5

Crude Drug Pkg. Size

Swertia Herb

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Theophylline

203-18281 209-18283 203-18281_209-18283

C7H8N4O2 = 180.16

CAS No. 58-55-9

Crude Drug Pkg. Size

Swertia Herb

100mg 1g
Grade Storage

for the JP Crude Drugs Test (for Determination)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Umbelliferone, 98.0+ % (HPLC)

212-01461

212-01461

C9H6O3 = 162.14

CAS No. 93-35-6

Crude Drug Pkg. Size

Artemisiae Folium

20 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

Product Name Wako Cat. No. Chemical Structural Formula

Wogonin, 98.0+ % (HPLC)

236-02321

236-02321

C16H12O5 = 284.26

CAS No. 632-85-9

Crude Drug Pkg. Size

Scutellariae Radix

10 mg

Grade Storage

for the JP Crude Drugs Test (for TLC)

Keep at 2-10°C.

indexpage top

HPLC columns used in analysis listed in this catalog

Functional group Packing material name Column Size Wako Cat. No. Purity of silica gel
D (DuPont type) W (Waters type)
Separation mode: Partition

ODS (C30)

Navi C30-5

4.6 φ x 150 mm

237-61191

233-61193

High purity

ODS (C18)

Navi C18-51397645907

4.6 φ x 150 mm

231-60511

237-60513

Wakosil-II 5C18

4.6 φ x 150 mm

230-51811

236-51813

Wakosil-II 5C18 AR

4.6 φ x 150 mm

238-50751

234-50753

Wakosil-II 5C18 HG

4.6 φ x 150 mm

238-51111

234-51113

Wakosil-II 5C18 RS

4.6 φ x 150 mm

238-51471

234-51473

Wakosil 5C18

4.6 φ x 150 mm

233-53981

239-53983

Normal

4.6 φ x 250 mm

237-54001

233-54003

Wakosil 5C18-2001397645907

4.6 φ x 250 mm

235-55521

231-55523

Ph (phenyl)

Wakosil 5Ph

4.6 φ x 250 mm

230-57411

236-57413

TLC plates used in analysis listed in this catalog

Product Name Package Size Wako Cat. No. Grade

Silicagel 70 Plate Wako

100 sheets ( 5 x 20 cm)

197-08384

for Thin-Layer
Chromatography

Silicagel 70 F254 Plate Wako

100 sheets ( 5 x 20 cm)

197-08404

Silicagel 70 PF254 Plate Wako

10 sheets (20 x 20 cm)

195-12871

Silicagel 70 FM Plate Wako

100 sheets ( 5 x 20 cm)

194-08394

Product Name Wako Cat. No. Chemical Structural Formula

Achyranthes Root

015-22271

unidentified

Crude Drug Pkg. Size

Achyranthis Radix

5 g

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC

Sample: Dissolve the 2 g in 10 mL of water. → Shake for 10 min. → Add 5 mL of
1-Butanol. → Shake for 10 min. → The supernatant after centrifugation.

Injection: 10 μL

Thin-layer plate: Silicagel plate for TLC

Developing solvent: Ethyl Acetate 5 + Water 1 + Formic Acid 1 (volume ratio) or
1-Propanol 4 + Ethyl Acetate 4 + Water 3 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT. → Spray diluted sulfuric acid.→ Dry at 105°C for 5 min.
→ Visual judgment.

Product Name Wako Cat. No. Chemical Structural Formula

Aconitine Standard, 99.0+ % (HPLC)

019-14621

019-14621

C34H47NO11 = 645.74

CAS No. 302-27-2

Crude Drug Pkg. Size

Aconiti Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 2 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: The upper layer of [Butyl Acetate 8 + 1-Propanol1 + Ammonia
Solution 1 (volume ratio)]

Distance spot travels: abt. 10 cm

Detection method: ① Spray Dragendorff's Reagent 1) → Visual judgment.
②UV exposure

1) Bismuth (III) Nitrate Basic 0.17 g + Acetic Acid 2 mL + Water 8 mL → Shake → Add
Potassium Iodide Solution (400 g/L) 10 mL → Shake

Sample: 1 mg/mL (Acetonitrile)

Injection: 10 μL

Column: Wakosil-II 5C18 AR, 4.6 mm φ x 15 cm
[Wako Cat. No.: 234-50753 (W), 238-50751 (D)] *

Column Temperature: Room temperature

Eluent: 0.05 mol/L Phosphate buffer 1) 68 + Acetonitrile 32 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 254 nm

1) [Disodium Hydrogen Phosphate 7.1 g + Water (→ 1 L)] + [Potassium Dihydrogen
Phosphate 6.8 g + Water (→ 1 L)] (→ pH 7.4).

Product Name Wako Cat. No. Chemical Structural Formula

Acteoside Standard 【Verbascoside】,
98.0+ % (HPLC)

013-24531

013-24531

C29H36O15 = 624.59

CAS No. 61276-17-3

Crude Drug Pkg. Size

Cistanche Herb

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 10 mg/2 mL (Methanol)

Sample spotted: 20 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Ethyl Acetate 20 + Methanol 3 + Water 2 (volume ratio)

Distance spot travels: abt. 7 cm

Detection method: Dry at RT. → Spray 2,6-Dibromo-N-Chloro-p-benzoquinone
Monoimine Solution 1). → Leave it in ammonia gas.→
Visual judgment.

1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.5 g + Methanol→ 100 mL

Sample: 2 mg/10 mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18HG,4.6 mm φ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.1 vol% Phosphoric Acid 85 + Acetonitrile 15 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 330 nm

Product Name Wako Cat. No. Chemical Structural Formula

Alisol B Acetate, 98.0+ % (HPLC)

018-13231

018-13231

C32H50O5 = 514.74

CAS No. 26575-95-1

Crude Drug Pkg. Size

Alismatis Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Toluene 55 + Acetone 35 + Hexane 10 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT. → Dry at 100°C for 10 min. → Allow to cool
→ Spray Sulfuric Acid‐Ethanol (99.5) solution (1+20) → Dry at RT
→ Heat at 200°C for 10 min. → Visual judgment.

Sample: 0.2 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18,4.6 mm φ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: Room temperature

Eluent: Acetonitrile 80 + Water 20 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 203 nm

Product Name Wako Cat. No. Chemical Structural Formula

Alisol B Standard, 99.0+ % (HPLC)

010-13431

010-13431

C30H48O4 = 472.70

CAS No. 18649-93-9

Crude Drug Pkg. Size

Alismatis Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Toluene 55 + Acetone 35 + Hexane 10 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Spray sulfuric acid (10 vol%)→ Heat at 120°C for 5 min.

Sample: 0.2 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18,4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column temperature: Room Temperature

Eluent: Acetonitrile 75 + Water 25 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 203nm

Product Name Wako Cat. No. Chemical Structural Formula

Alkannin Standard, 99.0+ % (HPLC)

013-19901

013-19901

C16H16O5 = 288.30

CAS No. 517-88-4

Crude Drug Pkg. Size

Lithospermum erythrorhizon

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Acetone)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)

Distance spot travels: abt.10 cm

Detection method: UV exposure and Visual judgment

Sample: 1 mg/mL (Methanol)

Injection: 5 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 2 vol% Phosphoric Acid solution 25 + Methanol 75(volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 273nm

Product Name Wako Cat. No. Chemical Structural Formula

Arctiin Standard, 98.0+% (HPLC)

019-24631

019-24631

C27H34O11 = 534.55

CAS No. 20362-31-6

Crude Drug Pkg. Size

Arctium lappa L.

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
HPLC

Sample: 5 mg/5 mL (Eluent)

Injection: 10μl

Column: Wakosil-II 5C18HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Water 75 + Acetonitrile 25 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 278nm

Product Name Wako Cat. No. Chemical Structural Formula

Artemisia Argyi

018-24221

Unidentified

?

Crude Drug Pkg. Size

Artemisiae Folium

10 g

Grade Storage

for the JP Crude Drugs Test

(for Purity Determination)

Keep at 2-10°C.

Example analysis
TLC

Sample: Add the 0.5 g in 5 mL of [Methanol 3 + Water 2 (volume ratio)].
→ Shake for 10 min. → Centrifuged supernatant.

Injection: 10 μL

Thin-layer plate: Octadecylsilanized silica gel for TLC

Developing solvent: Methanol 4 + Water 1 (volume ratio)

Distance spot travels: abt. 7 cm

Detection method: Dry at RT. → Spray diluted sulfuric acid.→ Dry at 105°C for 5
min. → UV exposure (dominant wavelength: 365 nm). → Green
fluorescing spots at around 0.3 and 0.4 of Rf value are confirmed as
identification of eupatilin and jaceosidin.

Product Name Wako Cat. No. Chemical Structural Formula

Atractylodin Standard, 98.0+ % (HPLC)

011-21531

011-21531

C13H10O = 182.22

CAS No. 55290-63-6

Crude Drug Pkg. Size

Atractylodis Lanceae

20 mg

Grade Storage

for Crude Drugs Determination

Keep at -80°C.

Example analysis
TLC HPLC

Sample: 20 μg/mL (Hexane)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Hexane 7 + Acetone 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT. → Spray p-dimethylaminobenzaldehyde solution.
→ Heat at 105°C for 5 min. → Visual judgment

Sample: 20 μg/mL (Methanol)

Injection: 20 μL

Column: Wakosil-II 5C18 RS, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *

Column Temperature: 40°C

Eluent: [Water 55 + Phosphoric Acid 1 (volume ratio)] 33 + Acetonitrile 67 (volume ratio)

Flow rate: 0.8 mL/min

Detector: UV 336 nm

Product Name Wako Cat. No. Chemical Structural Formula

Aucubin Standard, 97.0+ % (HPLC)

016-10351

016-10351

C15H22O9 = 346.33

CAS No. 479-98-1

Crude Drug Pkg. Size

Plantaginis Semen

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: The lower layer of [Chloroform 26 + Methanol 14
+ Water 5 (volume ratio)]

Distance spot travels: abt. 10 cm

Detection method: Spray [p‐Anisaldehyde 1g + Ethanol (99.5) 50 mL
+ Sulfuric Acid (1+5) 50 mL] → Heat → Visual judgment

Sample: 1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18, 4.6 mm φ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: Room Temperature

Eluent: Acetonitrile 3 + Water 97 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 206 nm

Product Name Wako Cat. No. Chemical Structural Formula

Baicalein Standard, 98.0+ % (HPLC)

027-07751

027-07751

C15H10O5 = 270.24

CAS No. 491-67-8

Crude Drug Pkg. Size

Scutellariae Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 2 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Acetic Acid 10 + Ethyl Acetate 70 + Methanol 30
+ Water 5 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (dominant wavelength: 254 nm)

Sample: 0.1 mg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 236-51813 (W), 230-51811 (D)] *

Column temperature: a constant temperature aroung room temperature

Eluent: 0.1 mol/L Phosphate buffer (pH2.1) 1) 40 + Methanol 60 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 280 nm

1) Potassium Dihydrogen Phosphate 2.72 g + Water 180 mL
→ Adjust the pH to 2.1 with Phosphoric Acid. + Water (→ 200 mL).

Product Name Wako Cat. No. Chemical Structural Formula

Bufotalin Standard, 99.0+ % (HPLC)

024-10691

024-10691

C26H36O6 = 444.57

CAS No. 471-95-4

Crude Drug Pkg. Size

Bufo bufo gargarizans Cantor

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Acetone 3 + Chloroform 3 + Cyclohexane 4 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Spray Cerium Sulfate (IV)·Sulfuric Acid (1+5) Solution (10 g/L)
→ Heat at 105°C for 5 min.

Sample: 0.5 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: Room temperature

Eluent: Acetonitrile 7 + Water 13 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 280 nm

Product Name Wako Cat. No. Chemical Structural Formula

Calycosin 7-O-Glucoside Standard,
98.0+ % (HPLC)

039-22271

039-22271

C22H22O10 = 446.4

CAS No. 20633-67-4

Crude Drug Pkg. Size

Astragali Radix

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
HPLC

Sample: 0.2 mg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18 RS, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *

Column Temperature: 40°C

Eluent: Water 850 + Acetonitrile 150 + Acetic Acid 1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 254 nm

Product Name Wako Cat. No. Chemical Structural Formula

Carbazochrome Sodium Sulfonate
Trihydrate,

99.0+ % (Calculated on the dehydrous basis)

039-21551

039-21551

C10H11N4NaO53H2O = 376.32

CAS No. 51460-26-5

Crude Drug Pkg. Size

Gardeniae Fructus

500 mg

Grade Storage

for Crude Drugs Determination

Keep at RT.

Example analysis
HPLC

Sample: 0.5 mg/mL (Water)

Injection: 10 μL

Column: ODS (particle size: 7 μm), 4.6 mmφ x 25 cm

Column Temperature: 40°C

Eluent: 10 mmol/L Phosphate buffer 1) 925 + Ethanol (95) 75 (volume ratio)
+ Phosphoric Acid (→ pH 3.0)

Detector: UV 360 nm

1) Ammonium dihydrogen phosphate 1.2 g + Water 1,000 mL

Product Name Wako Cat. No. Chemical Structural Formula

Catalpol Standard, 98.0+ % (HPLC)

032-10551

032-10551

C15H22O10 = 362.33

CAS No. 2415-24-9

Crude Drug Pkg. Size

Rehmanniae Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: The lower layer of [Chloroform 65 + Methanol 35
+ Water 10 (volume ratio)]

Distance spot travels: abt. 10 cm

Detection method: Spray Sulfuric Acid (1+10). → Heat → Visual judgment

Sample: 0.5 mg/mL (Eluent)

Injection:10 μL

Column: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: 35°C

Eluent: Acetonitrile 1 + Water 99 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 203 nm

Product Name Wako Cat. No. Chemical Structural Formula

Cinobufotalin Standard, 98.0+ % (HPLC)

035-13721

035-13721

C26H34O7 = 458.55

CAS No. 1108-68-5

Crude Drug Pkg. Size

Bufo bufo gargarizans Cantor

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Acetone 3 +Chloroform 3 +Cyclohexane 4 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Spray Cerium Sulfate (IV)·Sulfric Acid (1+5) Solution (1 w/v%)
→ Heat at 105°C for 5 min.→ Visual judgment.

Sample: 1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: Room Temperature

Eluent: Acetonitrile 40 + Water 60 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 270 nm

Product Name Wako Cat. No. Chemical Structural Formula

Corosolic Acid Standard, 98.0+ % (HPLC)

030-20741

030-20741

C30H48O4 = 472.70

CAS No. 4547-24-4

Crude Drug Pkg. Size

Lagerstroemia speciosa

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Chloroform 10 + Methanol 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT. → Spray Sulfuric Acid-Ethanol (99.5) solution (1+20).
→ Dry at RT.→ Heat at 130°C for 10 min.→ Visual judgment

Sample: 1 mg/mL (Methanol)

Injection: 20 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.1 vol% Trifluoroacetic Acid 35 + Acetonitrile 65 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 210nm

Product Name Wako Cat. No. Chemical Structural Formula

Corydaline Standard, 99.0+ % (HPLC)

036-14971

036-14971

C22H27NO4 = 369.45

CAS No. 518-69-4

Crude Drug Pkg. Size

Corydalis Tuber

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent:Acetone 3 +Chloroform 3 +Cyclohexane 4(volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Spray Cerium Sulfate (IV)·Sulfuric Acid (1+5) Solution (1 w/v%)
→ Heat at 105°C for 5 min. → Visual judgment

Sample: 1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: Room Temperature

Eluent: Acetonitrile 40 + Water 60 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 270 nm

Product Name Wako Cat. No. Chemical Structural Formula

Dehydrocostus Lactone Standard,

98.0+ % (HPLC)

042-32711

042-32711

C15H18O2 = 230.3

CAS No. 477-43-0

Crude Drug Pkg. Size

Saussureae Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 2 mg/ 2 mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Acetone 1 + Ethyl Acetate 2 + Hexane 7 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Spray Cerium Sulfate (IV)·Sulfuric Acid (1+5) Solution (10 g/L)
→ Heat at 105°C for 5 min.→ Visual judgment

Sample: 0.2 mg/mL (Eluent)

Injection:10 μL

Column: Wakosil-Ⅱ 5C18HG, 4.6 mmφ x 15 cm,
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 30°C

Eluent: Acetonitrile 60 + Water 40 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 215nm

Product Name Wako Cat. No. Chemical Structural Formula

Dihydrocapsaicin Standard,

99.0+ % (HPLC)

045-28911

045-28911

C18H29NO3 = 307.43

CAS No. 19408-84-5

Crude Drug Pkg. Size

Capsici Fructus

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 10 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Diethyl Ether 19 + Methanol 1 (volume ratio)

Distance spot travels: abt. 12 cm

Detection method: Spray 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine
Solution 1). → Leave it in ammonia gas.→ Visual judgment.

1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.1 g + Methanol 20 mL.

Sample: 0.2 mg/mL (Methanol)

Injection: 20 μL

Column: Wakosil 5Ph,4.6 mmφ x 25 cm
[Wako Cat. No.: 236-57413 (W), 230-57411 (D)] *

Column Temperature: 40°C

Eluent: 0.1 vol% Phosphoric Acid 50 + Acetonitrile 50 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 281 nm

Product Name Wako Cat. No. Chemical Structural Formula

5,7-Dimethoxyflavone Standard,
98.0+ % (HPLC)

040-32991

040-32991

C17H14O4 = 282.29

CAS No. 21392-57-4

Crude Drug Pkg. Size

Kaempferia parviflora,

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
HPLC

Sample: 0.1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 25 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Acetonitrile 55 + Water 45 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 260 nm

Product Name Wako Cat. No. Chemical Structural Formula

2,6-Dimethoxyphenol Standard

047-30681

047-30681

(CH3O)2C6H3OH = 154.16

CAS No. 91-10-1

Crude Drug Pkg. Size

-

5 g

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
Product Name Wako Cat. No. Chemical Structural Formula

Dimethylesculetin Standard

042-32331

042-32331

C11H10O4 = 206.19

CAS No. 120-08-1

Crude Drug Pkg. Size

Artemisiae Capillari Flos

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Hexane 50 + Acetone 50 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (Dominant wavelength: 365 nm)

Sample: 0.1 mg/mL (Acetonitrile)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 50°C

Eluent: Water 80 + Acetonitrile 20 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 230 nm

Product Name Wako Cat. No. Chemical Structural Formula

Echinacoside Standard, 98.0+ % (HPLC)

056-08601

056-08601

C35H46O20 = 786.73

CAS No. 82854-37-3

Crude Drug Pkg. Size

Cistanche Herb

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
HPLC

Sample: 5 mg/ 20 mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18 HG,4.6 mm φ x 25 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.1 vol% Phosphoric acid 85 + Acetonitrile 15 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 330 nm

Product Name Wako Cat. No. Chemical Structural Formula

Emetine Dihydrochloride Standard,
98.0+ % (HPLC)

059-08211

059-08211

C29H40N2O4·2HCl = 553.56

CAS No. 316-42-7

Crude Drug Pkg. Size

Carapichea ipecacuanha

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
HPLC

Sample: 10 mg/10ml (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 50°C

Eluent: Sodium 1-heptanesulfonate Solution 1) 50 + Methanol 50

(volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 283nm

1) Sodium 1-heptanesulfonate 2.0g+Water 500mL+Acetic Acid (→ pH4.0).

Product Name Wako Cat. No. Chemical Structural Formula

Epihesperidin Standard, 99.0+ % (HPLC)

085-08211

085-08211

C28H34O15 = 610.56

CAS No. 369593-42-0

Crude Drug Pkg. Size

Aurantii Nobilis Pericarpium

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 125 μg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 8 + Ethanol (99.5) 2 + Water 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (254 nm)

Sample: 125 μg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18,4.6 mm φ x 15 cm
[Wako Cat. No.: 236-51813 (W), 230-51811 (D)] *

Column Temperature: 50°C

Eluent: Water 82 + Acetonitrile 18 + Acetic Acid 1 (volume ratio)

Flow rate: 0.7 mL/min

Detector: UV 285 nm

Product Name Wako Cat. No. Chemical Structural Formula

Ergosterol Standard, 98.0+ % (HPLC)

052-04921

052-04921

C28H44O = 396.65

CAS No. 57-87-4

Crude Drug Pkg. Size

Polyporus

20 mg

Grade Storage

for Crude Drugs Determination

Keep at -20°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Chloroform)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Acetone 1 + Chloroform 1 + Toluene 10 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure

Sample: 0.4 mg/mL (Chloroform 1 + Methanol 1 (volume ratio))

Injection: 10 μL

Column: Wakosil 5C18, 4.6 mm φ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: RT

Eluent: Methanol

Flow rate: 1.0 mL/min

Detector: UV 254 nm

Product Name Wako Cat. No. Chemical Structural Formula

β-Eudesmol Standard, 98.0+ % (HPLC)

056-05161

056-05161

C15H26O = 222.37

CAS No. 473-15-4

Crude Drug Pkg. Size

Atractylodis Lanceae

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Acetone 1 + Ethyl Acetate 2 + Hexane 7 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Spray Cerium Sulfate (IV)-Sulfuric Acid (1+5) Solution (10 g/L).
→ Heat at 105°C for 5 min.

Sample: 0.5 mg/mL (Eluent)

Injection: 10 μL

Column: Navi C30-5, 4.6 mm φ x 15 cm
[Wako Cat. No.: 233-61193 (W), 237-61191 (D)] *

Column Temperature: 40°C

Eluent: Acetonitrile 60 + Water 40 (volume ratio)

Detector: UV 203 nm

Product Name Wako Cat. No. Chemical Structural Formula

Evodiamine Standard, 98.0+ % (HPLC)

051-08411

051-08411

C19H17N3O = 303.36

Crude Drug Pkg. Size

Evodiae Fructus

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Chloroform)

Injection: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Hexane 50 + Ethyl Acetate 50 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (dominant wavelength: 254 nm)

Sample: 0.2 mg/mL (Acetonitrile) 

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Acetonitrile 50 + 0.01 vol% Phosphoric Acid 50 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 270 nm

Product Name Wako Cat. No. Chemical Structural Formula

Geniposidic Acid Standard,

98.0+ % (HPLC)

078-05841

078-05841

C16H22O10 = 374.34

CAS No. 27741-01-1

Crude Drug Pkg. Size

·Gardeniae Fructus

·Plantaginis Semen

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Chloroform 13 + Methanol 7 + Water 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: ①UV exposure at 254 nm
②Spray [Vanillin 1 g
+ Ethanol (99.5) 50 mL + Sulfuric Acid(1+5) 50 mL]→ Heat at 105°C)
→ Visual judgment.

Sample: 0.2 mg/mL (Eluent)

Sample spotted: 10 μL

Column: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: 35°C

Eluent: 2 vol% Acetic Acid 95 + Acetonitrile 5 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 240 nm

Product Name Wako Cat. No. Chemical Structural Formula

[6]-Gingerol Standard, 98.0+ % (HPLC)

071-06051

071-06051

C17H26O4 = 294.39

Crude Drug Pkg. Size

Zingiberis Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 0.5 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Ethyl Acetate 1 + Hexane 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT.→ Spray 4-dimethylaminobenzaldehyde Solution 1).
→ Heat at 105°C for 5 min. → Allow to cool. → Visual judgment.

1) 4-dimethylaminobenzaldehyde 10g + chilled [Sulfuric Acid 90 mL + Water 10 mL]

Sample: 1 mg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 30°C

Eluent: Water 620 + Acetonitrile 380 + Phosphoric Acid 1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 282 nm

Product Name Wako Cat. No. Chemical Structural Formula

[8]-Gingerol Standard, 98.0+% (HPLC)

079-06231

079-06231

C19H30O4 = 322.44

CAS No. 23513-08-8

Crude Drug Pkg. Size

Zingiberis Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at -20°C.

Example analysis
HPLC

Sample: 5 mg/5 mL (Eluent)

Injection: 10 μL

Column: Wakosil-II5C18HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 30°C

Eluent: Water 50 + Acetonitrile 50 + Acetonitrile 0.1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 280nm

Product Name Wako Cat. No. Chemical Structural Formula

[10]-Gingerol Standard, 98.0+% (HPLC)

072-06221

072-06221

C21H34O4 = 350.49

CAS No. 23513-15-7

Crude Drug Pkg. Size

Zingiberis Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at -20°C.

Example analysis
HPLC

Sample: 5 mg/5 mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 30°C

Eluent: Water 40 + Acetonitrile 60 + Acetonitrile 0.1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 280nm

Product Name Wako Cat. No. Chemical Structural Formula

Ginsenoside-Rb1 Standard,

98.0+ % (HPLC)

079-02191

079-02191

C54H92O23 = 1109.29

CAS No. 41753-43-9

Crude Drug Pkg. Size

·Ginseng Radix Rubra

·Panacis Ginseng Radix

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: The lower layer of [Chloroform 13 + Methanol 7
+ Water 2 (volume ratio)]

Distance spot travels: abt. 10 cm

Detection method: Spray [p-Anisaldehyde 1 g + Ethanol (99.5) 50 mL + Sulfuric Acid
(1+5) 50 mL] → Heat → Visual judgment.

Sample: 0.5 mg/mL

Injection: 20 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Acetonitrile 35 + Water 65 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 203 nm

Product Name Wako Cat. No. Chemical Structural Formula

Ginsenoside-Rd Standard,

99.0+ % (HPLC)

072-03301

072-03301

C48H82O18 = 947.15

CAS No. 52705-93-8

Crude Drug Pkg. Size

·Ginseng Radix Rubra

·Panacis Ginseng Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: The lower layer of [Chloroform 13 + Methanol 7
+ Water 2 (volume ratio)]

Distance spot travels: abt. 10 cm

Detection method: Spray [p-methoxybenzaldehyde 1 g + Ethanol (99.5) 50 mL
+ Sulfuric Acid (1+5) 50 mL] → Heat → Visual judgment.

Sample: 0.5 mg/mL (Eluent)

Injection: 20 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Acetonitrile 35 + Water 65 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 203 nm

Product Name Wako Cat. No. Chemical Structural Formula

Glabridin, 97.0+ % (HPLC)

070-04821

070-04821

C20H20O4 = 324.37

CAS No. 59870-68-7

Crude Drug Pkg. Size

Glycyrrhizae Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Ethanol)

Injection: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent:Hexane 5 + Diethyl Ether 2 + Ethanol (99.5) 2 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (254nm)

Sample: 0.2 mg/mL (Eluent)

Injection: 20 μL

Column: Wakosil 5C18, 4.6 mmφ x 25 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: 40°C

Eluent: 2 vol% Acetic Acid 40 + Acetonitrile 60 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 282 nm

Product Name Wako Cat. No. Chemical Structural Formula

Glycocholic Acid Standard,
98.0+ % (HPLC)

072-05361

072-05361

C26H43NO6 = 465.62

CAS No. 475-31-0

Crude Drug Pkg. Size

Fel Ursi

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Toluene 10 + Acetic Acid 10 + Water 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT. → Spray Sulfuric Acid (1+18) → Heat at 105°C for 10 min.
→ Visual judgment

Sample: 0.45 mg/mL (Methanol)  

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 67 + Acetonitrile 33 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 200 nm

1) Potassium Dihydrogen Phosphate1.36g + Water 450 mL + Phosphoric Acid (→ pH 3.0)
+ Water (→ 500 mL)

Product Name Wako Cat. No. Chemical Structural Formula

Glycohyodeoxycholic Acid Standard,
98.0+ % (HPLC)

073-06251

073-06251

C26H43NO5 = 449.62

CAS No. 13042-33-6

Crude Drug Pkg. Size

·Powdered Swine Bile

·Fel Ursi

50 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 10 mg/5 mL (Methanol)

Sample spotted: 2 μL

Thin-layer plate: Silicagel 60 Plate, 20 x 5 cm

Developing solvent: Ethyl Acetate 12 + Methanol 2 + Formic Acid 1 (volume ratio)

Distance spot travels: abt. 7 cm

Detection method: Dry at RT. → Spray [Vanillin 3 g + Ethanol (99.5) 100 mL + Sulfuric
Acid 0.5 mL]→ Heat at 105°C for 10 min.→ Visual judgment.

Sample: 5 mg/ 5 mL (Methanol)  

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 64 + Acetonitrile 36 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 200 nm

1) Potassium Dihydrogen Phosphate 1.42g + Water 400 mL
+ Phosphoric Acid (→ pH 3.0) + Water (→ 500 mL)

Product Name Wako Cat. No. Chemical Structural Formula

Glycyrrhizic Acid Standard,

99.0+ % (HPLC)

071-02271

071-02271

C42H62O16 = 822.93

CAS No. 1405-86-3

Crude Drug Pkg. Size

Glycyrrhizae Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 2 mg/mL (Ethanol (50 vol%))

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: 1-Butanol 7 + Acetic Acid 1 + Water 2 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (254 nm)

Sample: 0.25 mg/mL (Ethanol (50 vol%))

Injection: 20 μL

Column: Wakosil 5C18-200, 4.6 mmφ x 25 cm
[Wako Cat. No.: 237-55503 (W), 231-55501 (D)] *

Column Temperature: 40°C

Eluent: Acetic Acid (6.7 vol%) 1) 3 + Acetonitrile 2 (volume ratio)

Flow rate: 0.8 mL/min

Detector: UV 254 nm

1) Acetic Acid 20 mL + Water (→ 300 mL).

Product Name Wako Cat. No. Chemical Structural Formula

Gomisin A Standard, 98.0+ % (HPLC)

077-04951

077-04951

C23H28O7 = 416.46

CAS No. 58546-54-6

Crude Drug Pkg. Size

Schisandrae Fructus

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 2.5 mg/mL (Acetonitrile)

Sample spotted: 1 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 10 + Hexane 10 + Acetic Acid 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV 254 nm

Sample: 1 mg/mL (Acetonitrile)

Injection: 5 μL

Column: Wakosil-II 5C18 HG,4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Methanol 60 + Water 40 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 254 nm

Product Name Wako Cat. No. Chemical Structural Formula

Gomisin N Standard, 98.0+ % (HPLC)

074-04961

074-04961

C23H28O6 = 400.46

CAS No. 69176-52-9

Crude Drug Pkg. Size

Schisandrae Fructus

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 2.5 mg/mL (Acetonitrile)

Sample spotted: 1 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 10 + Hexane 10 + Acetic Acid 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV 254 nm

Sample: 1 mg/mL (Acetonitrile)

Injection: 5 μL

Column: Wakosil-II 5C18 HG,4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Methanol 75 + Water 25 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 254 nm

Product Name Wako Cat. No. Chemical Structural Formula

Hypaconitine, 98.0+ % (HPLC)

084-07061

084-07061

C33H45NO10 = 615.72

CAS No. 6900-87-4

Crude Drug Pkg. Size

Aconiti Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep below 25°C.

Example analysis
TLC HPLC

Sample: 5 mg/ 5mL (Methanol)

Injection: 20 μL

Thin-layer plate: Silicagel 70FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure

Sample: 1 mg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.05 mol/L Phosphate buffer (pH 2.4) 1) 55 + Tetrahydrofuran 7
+ Acetonitrile 2 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 254 nm

1) Potassium Dihydrogen Phosphate1.36 g + Water 180 mL
→ Adjust the pH to 2.4 with Phosphoric Acid + Water (→ 200 mL).

Product Name Wako Cat. No. Chemical Structural Formula

Isoacteoside Standard, 98.0+ %(HPLC)

095-06671

095-06671

C29H36O15 = 624.59

CAS No. 61303-13-7

Crude Drug Pkg. Size

Cistanche Herb

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 10 mg/2 mL (Methanol)

Sample spotted: 20 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Ethyl Acetate 20 + Methanol 3 + Water 2 (volume ratio)

Distance spot travels: abt. 7 cm

Detection method: Dry at RT. → Spray 2,6-Dibromo-N-Chloro-p-benzoquinone
Monoimine Solution 1). → Leave it in ammonia gas.
→ Visual judgment.

1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.5 g + Methanol (→ 100 mL)

Sample: 2 mg/10 mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18HG,4.6 mm φ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.1 vol% Phosphoric Acid 85 + Acetonitrile 15 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 330 nm

Product Name Wako Cat. No. Chemical Structural Formula

Isofraxidine Standard, 99.0+ % (HPLC)

099-03651

099-03651

C11H10O5 = 222.19

CAS No. 486-21-5

Crude Drug Pkg. Size

Acanthopanax Root Bark

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 2 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Acetic Acid 1 + 1-Butanol 5 + Water 2 (volume ratio)

Distance spot travels: abt. 10 cm

Detection: UV exposure

Sample: 0.2 mg/mL (Methanol)

Injection: 5 μL

Column: Wakosil 5C18, 4.6 mm φ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: 40°C

Eluent: 0.1% Acetic Acid 80 + Acetonitrile 20 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 354 nm

Product Name Wako Cat. No. Chemical Structural Formula

(Z)-Ligustilide Standard Solution

(0.1 mg/mL Methanol Solution)

121-05801

121-05801

C12H14O2 = 190.24

CAS No. 4431-01-0

Crude Drug Pkg. Size

Angelicae Radix

500 μL x 5A

Grade Storage

for Crude Drugs Determination

Keep at -20°C.

Example analysis
TLC HPLC

Sample spotted: 1 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 1 + Hexane 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection: UV exposure

Injection: 20 μL

Column: Wakosil-II 5C18 RS, 4.6 mm φ x 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *

Column Temperature: 40°C

Eluent: Phosphoric Acid (1+55) 40 + Acetonitrile 60 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 326 nm

Product Name Wako Cat. No. Chemical Structural Formula

Magnoflorine Standard, 95.0+% (HPLC)

138-17201

138-17201

C20H24NO4 = 342.41

CAS No. 2141-09-5

Crude Drug Pkg. Size

Magnoliae Flos

5 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Ethyl Acetate 5 + Acetone 3 + Water 1 + Formic Acid 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Air drying → Spray Dragendorff's Reagent 1) → Visual judgment.

1) Bismuth Nitrate Basic 0.85 g + Acetic Acid 10 mL + Water 40 mL (Dissolve by
shaking vigorously)(= Soln. A). Potassium Iodide 8 g + Water 20 mL (= Soln. B).
Just before use, mix equal part of Soln. A and Soln. B. Take the 4 mL of the mixture
and add 20 mL of 1 mol/L Acetic Acid.

Sample: 0.2 mg/mL (Methanol)

Injection: 20 μL

Column: Wakosil-II 5C18 RS, 4.6 mm φ x 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *

Column Temperature: 40°C

Eluent: SDS Solution 1) 65 + Acetonitrile 35 + Phosphoric Acid 0.1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 270 nm

1) Sodium Dodecyl Sulfate 1 g + Water (→ 130 mL).

Product Name Wako Cat. No. Chemical Structural Formula

Magnoflorine Iodide Standard,

95.0+% (qNMR)

138-17941

138-17941

C20H24INO4 = 469.31

CAS No. 4277-43-4

Crude Drug Pkg. Size

Magnoliae Flos

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg + 1 mL of [Methanol 1 + Water 1] → ultrasonic irradiation + 2 mL of
[Methanol 1 + Water 1]

Sample spotted: 10 μL

Thin-layer plate: Silicagel 60, 20 x 5 cm

Developing solvent: Ethyl Acetate 5 + Acetone 3 + Water 1 + Formic Acid 1 (volume ratio)

Distance spot travels: abt. 7 cm

Detection method: Dry at RT.→ Spray Dragendorff Solution 1). → Dry at RT.→ Spray
Sodium Nitrite Solution 2)→ Visual judgment.

1) Bismuth Subnitrate 0.85 g + Acetic Acid 10 mL +Water 40 mL (Beat Up)
→ [Soln. C]. Potassium Iodide 8 g + Water 20 mL → [Soln. D].
Soln. C 2 mL + Soln. D 2 mL + 1 mol/L Acetic Acid 20 mL (Prepare these solutions
just before use.)

2) Sodium Nitrite 10 g + Water (→ 100 mL)

Sample: 5 mg + 5 mL of [Methanol 1 + Water 1] → Ultrasonic Irradiation
→ Add [Methanol 1 + Water 1] to prepare the 10 mL soln.

Injection: 10 μL

Column: Wakosil-II 5C18 RS, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *

Column Temperature: 40°C

Eluent: Sodium Lauryl Sulfate 5 g + Acetonitrile 350 mL + Water 650 mL
+ Phosphoric Acid 1 mL (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 303 nm

Product Name Wako Cat. No. Chemical Structural Formula

Mesaconitine, 98.0+% (HPLC)

130-12261

130-12261

C33H45NO11 = 631.71

CAS No. 2752-64-9

Crude Drug Pkg. Size

Aconiti Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep below 25°C.

Example analysis
TLC HPLC

Sample: 2 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method : UV exposure

Sample: 1 mg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mm φ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.05 mol/L Phosphate buffer (pH 2.4) 1) 90 + Tetrahydrofuran 10 (volume ratio)

Flow rate: 1.2 mL/min

Detector: UV 236 nm

1) Potassium Dihydrogen Phosphate1.36 g + Water 180 mL→ Adjust the pH to 2.4 with
Phosphoric Acid + Water (→ 200 mL).

Product Name Wako Cat. No. Chemical Structural Formula

Neohesperidin Standard, 98.0+% (HPLC)

142-09191

142-09191

C28H34O15 = 610.56

CAS No. 13241-33-3

Crude Drug Pkg. Size

immature orange

10 mg

Grade Storage

for Crude Drugs Determination

Keep at -20°C.

Example analysis
TLC HPLC

Sample: 2 mg / 2 mL (Methanol)

Sample spotted: 20 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent:Ethyl Acetate 7 + 1-Propanol 5 + Water 4 + Acetic Acid 1 (volume
ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT.→ Spray 2,6-Dibromo-N-Chloro-1,4-benzoquinone
Monoimine Solution 1). → Leave it in ammonia gas.→ Visual
judgment.

1) 2,6-Dibromo-N-Chloro-1,4-benzoquinone Monoimine 0.5 g + Methanol (→ 100 mL).

Sample: 2 mg / 10mL (Methanol)

Injection: 10 μL

Column: Wakopak Navi C18-5,4.6 mm φ x 15 cm
[Wako Cat. No.: 237-60513 (W),231-60511 (D)] *

Column Temperature: 40°C

Eluent: Water 82 + Acetonitrile 18 + Acetic Acid 1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 285 nm

Product Name Wako Cat. No. Chemical Structural Formula

γ-Oryzanol Standard,

98.0+% (molecular absorptiometric analysis)

156-02831

Mixture of steryl ferulates

?

Crude Drug Pkg. Size

Oryzae Semen

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC Molecular absorptiometric analysis

Sample: 2.5 mg/mL (Ethyl Acetate)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 60 F254, 20 x 5 cm

Developing solvent: Hexane 5 + Acetone 2 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV 365 nm

Sample: 10μg/mL (Heptane 1))

Detection method: Measure the absorption maximum wavelength around 315 nm.

1) for HPLC

Product Name Wako Cat. No. Chemical Structural Formula

Oxymatrine Standard, 98.0+% (HPLC)

150-01511

150-01511

C15H24N2O2 = 264.37

CAS No. 16837-52-8

Crude Drug Pkg. Size

Sophorae Radix

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Chloroform1 + Methanol 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT.→ Dry at 80°C for 10 min.→ Allow to cool.→ Spray
Dragendorff's Reagent 1)→ Visual judgment.

1) Bismuth Nitrate Basic 0.17 g + Acetic Acid 2 mL + Water 8 mL→ Shake + Potassium
Iodide Solution (400 g/L) 10 mL→ Shake.

Sample: 0.2 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: 35°C

Eluent: [0.02 mol/L Phosphate buffer 1) 65 + Acetonitrile 35 (volume ratio)]

400 mL + Sodium Dodecyl Sulfate 0.2 g

Flow rate: 1.0 mL/min

Detector: UV 220 nm

1) Potassium Dihydrogen Phosphate 0.81g + Water (→ 300 mL)
→ Adjust the pH to 2.5 with Phosphoric Acid.

Product Name Wako Cat. No. Chemical Structural Formula

Plantago Seed

166-23751

Unidentified

?

Crude Drug Pkg. Size

Plantaginis Semen

10 g

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC

Sample: Add the 2 g in 3 mL of Methanol and heat for 3 min. in a water bath. After
cooling, centrifuge followed by taking the supernatant. 

Sample spotted: 10 μL

Thin-layer plate: Silicagel plate for TLC

Developing solvent: Acetone 10 + Ethyl Acetate 10 + Water 3 + Acetic Acid (100)
1 (volume ratio) or Ethyl Acetate 6 + Water 1 + Formic acid 1
(volume ratio)

Distance spot travels: abt. 10 cm

Detection method: After air drying, spray 4-methoxybenzaldehyde · Sulfuric Acid
reagent. → Heat at 105°C for 10 min. → Visual judgment

Product Name Wako Cat. No. Chemical Structural Formula

Praeruptorin A Standard, 98.0+% (HPLC)

168-22231

168-22231

C21H22O7 = 386.4

CAS No. 73069-25-7

Crude Drug Pkg. Size

Peucedani Radix

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Diethyl Ether 3 + Hexane 1 (volume ratio)

Distance spot travels : abt. 10 cm

Detection method: UV 365 nm

Sample: 1 mg/mL (Methanol)

Injection: 5 μL

Column: Wakopak Navi C18-5, 4.6 mmφ x 15 cm
[Wako Cat. No.: 237-60513 (W),231-60511 (D)] *

Column Temperature: 40°C

Eluent: Water 45 + Acetonitrile 55 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 322 nm

Product Name Wako Cat. No. Chemical Structural Formula

Rhaponticin Standard, 98.0+% (HPLC)

189-02721

189-02721

C21H24O9 = 420.41

CAS No. 155-58-8

Crude Drug Pkg. Size

Rhei Rhizoma

5 mg

Grade Storage

for Crude Drugs Determination

Keep at -20°C.

Example analysis
TLC HPLC

Sample: 2 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Devedloping Solvent: Ethyl Formate 10 + 2-Butanone 7 + Formic Acid 1
+ Water 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure (dominant wavelength: 365 nm)

Sample: 0.2 mg/mL (Methanol)

Sample spotted: 5 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 0.1 vol% Phosphoric Acid 65 + Methanol 35 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 324 nm

Product Name Wako Cat. No. Chemical Structural Formula

Rutin Standard, 95.0+% (HPLC)

188-02791

188-02791

C27H30O16 = 610.52

CAS No. 153-18-4

Crude Drug Pkg. Size

Crataegi Fructus

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Methanol)

Sample spotted: 2 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Ethyl Acetate 5 + 2-Butanone 3 + Water 1 + Formic Acid 1 (volume
ratio)

Distance spot travels: abt. 10 cm

Detection method: After air drying, spray diluted sulfuric acid.→ After heating at 105°C
for 5 min, expose to UV (dominant wavelength: 365 nm)

Sample: 0.4 mg/mL (Methanol)

Injection: 5 μL

Column: Wakosil 5C18,4.6 mm φ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: 40°C

Eluent: Water 70 + Methanol 30 + Acetic Acid 5 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 254nm

Product Name Wako Cat. No. Chemical Structural Formula

Saikosaponin c Standard, 99.0+% (HPLC)

197-08421

197-08421

C48H78O17 = 927.12

CAS No. 20736-08-7

Crude Drug Pkg. Size

Bupleuri Radix

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: The lower layer of [Chloroform 7 + Methanol 3
+ Water 1 (volume ratio)]

Distance spot travels: abt. 10 cm

Detection method: Spray [p-Anisaldehyde 1 g + Ethanol (99.5) 50 mL
+ Sulfuric Acid (1+5) 50 mL].→ Heat → Visual judgment.

Sample: 0.5 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18 HG,4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 35°C

Eluent: Acetonitrile 35 + Water 65 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 203 nm

Product Name Wako Cat. No. Chemical Structural Formula

Scopolamine Hydrobromide n-Hydrate
Standard, 99.0+% (HPLC)

190-16341

190-16341

C17H21NO4·HBr = 384.26

CAS No. 114-49-8

Crude Drug Pkg. Size

Scopoliae Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 0.6 mg/mL (Ethanol (99.5))

Sample spotted: 10 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Methanol 200 + 28% Ammonia Solution 3 (volume ratio)

Distance spot travels : abt. 10 cm

Detection method: Dry at RT.→ Spray Dragendorff's Reagent 1) → Visual judgment.

1) Bismuth (III) Nitrate Basic 0.17g + Acetic Acid 2 mL+ Water 8 mL → Shake +
Potassium Iodide Soln. (400 g/L) 10 mL→ Shake.

Sample: 1.1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Phosphoric Acid·Triethylamine buffer 1) 9 + Acetonitrile 1 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 210 nm

1) Potassium Dihydrogen Phosphate 6.8 g + Water 900 mL + Triethylamine 10 mL
+ Phosphoric Acid (→ pH 3.5) + Water (→ 1 L).

Product Name Wako Cat. No. Chemical Structural Formula

Sennoside A Standard, 98.0+% (HPLC)

194-16241

194-16241

C42H38O20 = 862.74

CAS No. 81-27-6

Crude Drug Pkg. Size

·Sennae Folium

·Rhei Rhizoma

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Tetrahydrofuran 7 + Water 3 (volume ratio))

Sample spotted: 20 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Ethyl Acetate 40 + 1-Propanol 40 + Water 30
+ Acetic Acid 1 (volume ratio)

Distance spot travels:abt. 15 cm

Detection method: UV 365 nm

Sample: 0.1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 50°C

Eluent: [Acetic Acid・Sodium Acetate buffer (pH 5.0) 1) 17 + Acetonitrile 8 (volume ratio)] 1 L
+ Tetra-n-heptylammonium Bromide 2.45 g

Flow rate: 0.7 mL/min

Detector: UV 340 nm

1) [Sodium Acetate Trihydrate 13.6 g + Water ( → 100 mL)] + [Acetic Acid 6 g
+ Water (→ 100 mL)](→ pH 5.0) → Take the 100 mL + Water (→ 1 L).

Product Name Wako Cat. No. Chemical Structural Formula

Sennoside B Standard, 98.0+% (HPLC)

197-16091

197-16091

C42H38O20 = 862.74

CAS No. 128-57-4

Crude Drug Pkg. Size

·Sennae Folium

·Rhei Rhizoma

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Tetrahydrofuran7 + Water 3 (volume ratio))

Sample spotted: 20 μL

Thin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]

Developing solvent: Ethyl Acetate 7 + 1-Propanol 7 + Water 4
+ Formic Acid 2 (volume ratio)

Distance spot travels: abt. 15 cm

Detection method: UV 365 nm

Sample: 0.1 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 50°C

Eluent: [Acetic Acid・Sodium Acetate buffer (pH 5.0) 1) 17 + Acetonitrile 8 (volume ratio)] 1 L
+ Tetra-n-heptylammonium Bromide 2.45 g

Flow rate: 0.7 mL/min

Detector: UV 340 nm

1) [Sodium Acetate Trihydrate 13.6 g + Water ( → 100 mL)] + [Acetic Acid 6 g
+ Water ( → 100 mL)](→ pH 5.0) → Take the 100 mL + Water (→ 1 L).

Product Name Wako Cat. No. Chemical Structural Formula

Shikonin (mixture of optical isomers, abt. 6 : 1),
98.0+% (HPLC)

198-13581

198-13581

C16H16O5 = 288.30

CAS No. 517-89-5

Crude Drug Pkg. Size

Lithospermum erythrorhizon

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Acetone)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Visual judgment and UV exposure

Sample: 1 mg/mL (Methanol)

Injection: 5 μL

Column: CHIRALCEL OJ-H, 4.6 mmφ x 25 cm

Column Temperature: 40°C

Eluent: Hexane 95 + 2-Propanol 5 + Acetic Acid 0.3 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 273 nm

Product Name Wako Cat. No. Chemical Structural Formula

Shikonin (mixture of optical isomers, abt. 1 : 1),
98.0+% (HPLC)

198-13601

198-13601

C16H16O5 = 288.30

CAS No. 54952-43-1

Crude Drug Pkg. Size

Lithospermum erythrorhizon

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC (1)

Sample: 5 mg/mL (Acetone)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: ①Visual judgment ②UV exposure

Sample: 1 mg/mL (Methanol)

Injection: 5 μL

Column: CHIRALCEL OJ-H, 4.6 mmφ x 25 cm

Column Temperature: 40°C

Eluent: Hexane 95 + 2-Propanol 5 + Acetic Acid 0.3 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 273 nm

HPLC (2)

Sample: 1mg/mL (Methanol)

Injection: 5 μL

Column: Wakosil-II 5C18 HG,4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 2 vol% Phosphoric Acid 25 + Methanol 75 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 273 nm

Quantitation: Percentage by area

Product Name Wako Cat. No. Chemical Structural Formula

Shikonin Standard, 99.0+% (HPLC)

191-13331

191-13331

C16H16O5 = 288.30

CAS No. 517-89-5

Crude Drug Pkg. Size

Lithospermum erythrorhizon

10 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 5 mg/mL (Acetone)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure and visual judgment

Sample: 1 mg/mL (Methanol)

Injection: 5 μL

Column: Wakosil-II 5C18 HG,4.6 mm x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 2 vol% Phosphoric Acid solution 25 +Methanol 75 (volume ratio)

Flow rate: 1.0 mL/min.

Detector: UV 273nm

Product Name Wako Cat. No. Chemical Structural Formula

Sinomenine Standard, 98.0+% (HPLC)

193-09361

193-09361

C19H23NO4 = 329.39

CAS No. 115-53-7

Crude Drug Pkg. Size

Sinomeni Caulis et Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 10 mg/mL (Chloroform)

Sample spotted: 2 μL

Thin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]

Developing solvent: Chloroform 20 + Methanol 20
+ Ammonia Solution 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: UV exposure

Sample: 0.2 mg/mL (Eluent)

Injection: 10 μL

Column: Wakosil 5C18,4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *

Column Temperature: Room temperature

Eluent: Sodium Dodecyl Sulfate Solution (5 g/L) 11 + Acetonitrile 9 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 260 nm

Product Name Wako Cat. No. Chemical Structural Formula

Swertiamarin Standard, 98.0+% (HPLC)

198-16901

198-16901

C16H22O10 = 374.34

CAS No. 17388-39-5

Crude Drug Pkg. Size

Swertia Herb

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
HPLC

Sample: 2 mg/ 10 mL (Eluent)

Injection: 10μL

Column: Wakosil-II 5C18HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Acetonitrile 10 + Water 90 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 238nm

Product Name Wako Cat. No. Chemical Structural Formula

Tauroursodeoxycholic Acid Standard,
98.0+% (HPLC)

203-17061

203-17061

C26H45NO6S = 499.70

CAS No. 14605-22-2

Crude Drug Pkg. Size

Fel Ursi

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 10 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: Toluene 10 + Acetic Acid 10 + Water 1 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method: Dry at RT.→ Spray Sulfuric Acid (1+18) → Heat at 105°C for 10 min.
→ Visual judgment.

Sample: 0.5 mg/mL (Methanol)

Injection: 10 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 70 + Acetonitrile 30 (volume ratio)

Flow rate: 1.0 mL/min

Detector: UV 200 nm

1) Potassium Dihydrogen Phosphate1.36 g + Water 450 mL
+ Phosphoric Acid (→ pH 3.0) + Water (→ 500 mL).

Product Name Wako Cat. No. Chemical Structural Formula

Timosaponin A-III Standard,

98.0+% (HPLC)

207-16861

207-16861

C39H64O13 = 740.92

CAS No. 41059-79-4

Crude Drug Pkg. Size

Anemarrhenae Rhizoma

20 mg

Grade Storage

for Crude Drugs Determination

Keep at 2-10°C.

Example analysis
TLC HPLC

Sample: 1 mg/mL (Methanol)

Sample spotted: 5 μL

Thin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]

Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)

Distance spot travels: abt. 10 cm

Detection method : Dry at RT.→ Spray [Sulfuric Acid 1 + Ethanol (99.5) 20 (volume
ratio)]→ Dry at RT. → Heat at 105°C for 5 min.→ Visual judgment.

Sample: 10 mg/mL (DMSO 3 + Methanol 7)

Sample spotted: 20 μL

Column: Wakosil-II 5C18 HG, 4.6 mmφ x 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *

Column Temperature: 40°C

Eluent: Water 50 + Acetonitrile 50 (volume ratio)

Flow rate: 1.0 mL/min

Detector: Differential refractometer detector

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index

A
Achyranthes RootAconitine StandardAconitum Diester Alkaloids StandardAconitum Monoester Alkaloids StandardActeoside Standard AlbiflorinAlisol AAlisol B AcetateAlisol B StandardAlkannin StandardAmygdalinArbutinArctiin StandardArtemisia Argyi Artemisia ArgyiAsarinin(E)-AsaroneAstragaloside IVAtractylenolide IIIAtractylodin StandardAtractylodin TSAtropine Sulfate HydrateAucubin Standard
B
Baicalein StandardBaicalinBarbaloinBenz [a] anthracene Benzo [a] pyreneBenzoylmesaconine HydrochlorideBerberine Chloride HydrateBergeninBisdemethoxycurcuminBufalinBufotalin Standard
C
Calycosin 7-O-Glucoside Standard(E)-CapsaicinCarbazochrome Sodium Sulfonate TrihydrateCatalpol StandardChikusetsusaponin IV(E)-Chlorogenic Acid(E)-Cinnamaldehyde(E)-Cinnamic AcidCinobufaginCinobufotalin StandardCoptisine ChlorideCorosolic Acid StandardCorydaline StandardCurcuminCycloartenyl Ferulate
D
Dehydrocorydaline Nitrate Dehydrocostus Lactone StandardDemethoxycurcuminDihydrocapsaicin Standard5,7-Dimethoxyflavone Standard2,6-Dimethoxyphenol StandardDimethylesculetin Standard
E
Echinacoside StandardEleutheroside BEmetine Dihydrochloride StandardEpihesperidin StandardErgosterol Standardβ-Eudesmol StandardEvodiamine Standard
F
(E)-Ferulic AcidFructose
G
GeniposideGeniposidic Acid StandardGentiopicroside[6]-Gingerol[6]-Gingerol Standard[8]-Gingerol Standard[10]-Gingerol StandardGinsenoside ReGinsenoside-Rb1 StandardGinsenoside-Rd StandardGinsenoside RcGinsenoside-Rg1Glabridin4'-O-Glucosyl-5-O-methylvisamminolGlycocholic Acid StandardGlycohyodeoxycholic Acid StandardGlycyrrhizic AcidGlycyrrhizic Acid StandardGomisin A StandardGomisin N StandardGuaiacol
H
HesperidinHirsutineHonokiol10-Hydroxy-2-(E)-decenoic Acid3-(3-Hydroxy-4-methoxyphenyl)-2-(E)-propenoic AcidHyodeoxycholic AcidHypaconitineHyperoside
I
IcariinIsoacteoside StandardIsofraxidine Standard
L
(Z)-Ligustilide Standard SolutionLimoninLiquiritinLoganinLuteolin
M
Magnoflorine Iodide StandardMagnoflorine StandardMagnololManninotriosel-MentholMesaconitine(E)-2-MethoxycinnamaldehydeMyristicin
N
NaringinNeohesperidin StandardNodakenin
O
γ-Oryzanol StandardOstholeOxymatrine Standard
P
PaeoniflorinPaeonolPalmatine ChloridePerillaldehydePlantago SeedPlantago SeedPowdered Swine BilePraeruptorin A Standard(±)-Praeruptorin APuerarin
R
ResibufogeninRhaponticin StandardRheinRhynchophyllineRosmarinic AcidRutin Standard
S
Saikosaponin aSaikosaponin b2Saikosaponin c StandardSaikosaponin dSchizandrinScopolamine Hydrobromide n-Hydrate StandardScopoletinSennoside A StandardSennoside B StandardSesaminShikonin StandardShikoninShikonin[6]-ShogaolSinomenine StandardSodium GlycocholateSodium TauroursodeoxycholateStachyoseSwertiamarinSwertiamarin Standard
T
Tauroursodeoxycholic Acid StandardTheophyllineTimosaponin A-III Standard
U
Umbelliferone
V
Verbascoside
W
Wogonin
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