Phos-tag™ is …
- 【Basic Structure of Phos-tag™】
- M2+：Zinc ion or manganese ion
- ◇ Selectivity of binding of a phosphate ion (2-) is much higher than that of other anions.
- ◇ Stable complex is formed under physiological conditions (pH 5 to 8).
|Product||Purpose of Use|
|Phos-tag™ Acrylamide||Separation：Separation is possible by SDS-PAGE depending on the degree of phosphorylation.|
|SuperSep Phos-tag™||Separation：Ready-to-Use Precase gel containing 50 µM Phos-tag™ Acrylamide|
|Phos-tag™ Biotin||Detection：A substitute for the anti-phospho antibody used in western blot.|
|Phos-tag™ Agarose||Purification：Phosphorylated proteins are purified by column chromatography.|
Mass Analytical Kit
|Analysis：This is used in MALDI-TOF/MS analysis to improve the detection sensitivity of phosphorylated molecules.|
Phos-tag™ was developed by Department of Functional Molecular Science at Hiroshima University. http://www.phos-tag.com/
Two metallic ions cooperate to bind a phosphate group
Phosphorylated proteins move while being bound by Phos-tag™ in the gel
Migration speed of phosphorylated proteins decreases and they are separated from non-phosphorylated proteins.
Application ― Time course of phosphorylation by using the Tyrosin Kinase Abl ―
Conventional SDS-PAGE（CBB staining）
Phos-tag™ SDS-PAGE（CBB staining）