Phos-tag™ PAGE GUIDEBOOK

Phos-tag™ Acrylamide is …

Phos-tag™ Acrylamide

Phos-tag™ Acrylamide is an acrylamide-pendant Phos-tag™ molecule. Phos-tag™ SDS-PAGE, where a polyacrylamide gel containing Phos-tag™ is used, can be prepared by adding Phos-tag™ Acrylamide and MnCl2 in Resolving Gel when preparing SDS-PAGE gels. During migration, the phosphorylated proteins with the phosphate group bound to the divalent metal ions in Phos-tag™. This decreases the migration speed and phosphorylaed/non-phosphorylated proteins are separated. After separation, the gel can be utilized for western blotting and mass spectrometry. Various Phos-tag™ SDS-PAGE precast gels are also available. Please see 8.SuperSep Phos-tag™ on the page #19 for the details.

By simply adding Phos-tag™ Acrylamide and MnCl2 to an acrylamide solution when preparing SDS-PAGE gels Phosphorylated proteins are easily separable.

By SDS-PAGE

Time-course analysis of β-casein using Phos-tag™ SDS-PAGE
Degree of phosphorylation

Development from Phos-tag™ SDS-PAGE

By a combination Phos-tag™ SDS-PAGE and various analysis methods, new information of phosphorylated proteins can be obtained.

Western blotting

Easily-recognizable phosphorylation of your target proteins Simultaneous detection of phosphorylated/non-phosphorylated proteins with a general antibody by their band shift differences. No need to prepare the phosphorylated antibody. Applicable to analysis of phosphorylation of endogenous proteins. Application Data
→ See the page.

Western blotting

Mass Analysis

By separating phosphorylated forms, each phosphorylation site combination can be known.

2D Electrophoresis

Phosphorylated forms with the same isoelectric points (same number of phosphorylation sites) can be separated. Application Data
→ See the page

Phos-tag™

Product Name Pkg. Size Wako Cat. No.
(Nard Product #)
Application
Phos-tag™ Acrylamide 5 mM Aqueous Solution 0.3 mL
(0.9 ㎎)
304-93526(AAL-107S1) Separation of phosphorylated and non-phosphorylated proteins using SDS-PAGE with Phos-tag™ Acrylamide
Phos-tag™ Acrylamide 2 ㎎ 300-93523(AAL-107M)
10 ㎎ 304-93521(AAL-107)