By simply adding Phos-tag™ Acrylamide and MnCl2 to an acrylamide solution when preparing SDS-PAGE gels Phosphorylated proteins are easily separable.
- Time-course analysis of β-casein using Phos-tag™ SDS-PAGE
Development from Phos-tag™ SDS-PAGE
Easily-recognizable phosphorylation of your target proteins Simultaneous detection of phosphorylated/non-phosphorylated proteins with a general antibody by their band shift differences. No need to prepare the phosphorylated antibody. Applicable to analysis of phosphorylation of endogenous proteins. Application Data
→ See the page.
By separating phosphorylated forms, each phosphorylation site combination can be known.
Phosphorylated forms with the same isoelectric points (same number of phosphorylation sites) can be separated. Application Data
→ See the page
|Product Name||Pkg. Size||Wako Cat. No.
(Nard Product #)
|Phos-tag™ Acrylamide 5 mM Aqueous Solution||0.3 mL
|304-93526(AAL-107S1)||Separation of phosphorylated and non-phosphorylated proteins using SDS-PAGE with Phos-tag™ Acrylamide|
|Phos-tag™ Acrylamide||2 ㎎||300-93523(AAL-107M)|