Phos-tag™ PAGE GUIDEBOOK

SuperSep Phos-tag™

SuperSep Phos-tag™ is a precast gel, which can be immediately used after opening the package, is added with 50 μmol/L of Phos-tag™ Acrylamide in advance. It contains zinc as a supplemental metal. It has excellent storage stability by its neutral gel buffer. Sharp bands can be obtained.

Features

œ Ready-to-use

œ Safety due to precast gel

œ Long-term stability (Stable for 6 months)

œ Almost the same basic mechanism as that of SDS-PAGE

Plate Size 100 x 100 x 3 (mm)
Gel Size 90 x 85 x 1 (mm)
Well number 13 17
Phos-tag™ conc. 50 μmol/L
Acrylamide conc. 12.5% or 15%
ZnCl2 conc. 100 μmol/L
Well volume 30 μL 25 μL

SuperSep Phos-tag™/EasySeparator

ATTENTION

EThis product is a precast gel optimized in an gEasySeparatorh tank.

EUse of a normal prestained marker will distort the bands. Use of WIDE-VIEW™ Prestained Protein Size Marker Ⅲ (Wako Cat. No. 230-02461) is recommended. Please refer to gPhos-tag™ Acrylamide - Molecular Markerhin gSec. 7. FAQ.h on the page.

EBefore using this product, check a sample for migration pattern problems with gSuperSep Aceh or other normal SDS-PAGE.

EWhen performing Western blotting, execute an EDTA process before transfer. For details, refer to gSec. 4. Troubleshooting.h

Application ` Dephosphorylation over time of β-casein `

SuperSep Ace, 12.5%, 13 wells/SuperSep Phos-tag™, 12.5%, 13 wells

[ Buffer ] Tris-Glycine-SDS electrophoresis buffer

[ Sample ] M: WIDE-VIEW™ Prestained Protein Size Marker Ⅲ (Wako Cat.No. 230-02461)

1 : 0 min. β-casein (with AP treatment)

2 : 15 min. β-casein (with AP treatment)

3 : 30 min. β-casein (with AP treatment)

4 : 45 min.β-casein (with AP treatment)

5 : 60 min. β-casein (with AP treatment)

[ Condition ] Constant current 35 mA for 60 min.

[ Staining ] Quick-CBB StainingiWako Cat. No. 299-50101j

[ Destaining ] Deionized water with microwave

β-casein was dephosphorylated over time. Dephosphorylated β-casein can be separated from β-casein with SuperSep Phos-tag™.

Product Name PackageSize Wako Cat. No. Storage
SuperSep Phos-tag™ Precast gels for Mini-PROTEAN® Tetra Cell (Bio-Rad's electrophoresis tank)
NEWSuperSep Phos-tag™ (50 μmol/L), 7.5%, 17 well, 83 x 100 x 3.9 mm 5 gels 198-17981 Keep at 2~10℃
NEWSuperSep Phos-tag™ (50 μmol/L), 12.5%, 17 well, 83 x 100 x 3.9 mm 5 gels 195-17991
SuperSep Phos-tag™ Precast gels for XCell SureLock® Mini-Cell (Life Technologies's electrophoresis tank)
NEWSuperSep Phos-tag™ (50 μmol/L), 7.5%, 17 well, 100 x 100 x 6.6 mm 5 gels 192-18001 Keep at 2~10℃
NEWSuperSep Phos-tag™ (50 μmol/L), 12.5%, 17 well, 100 x 100 x 6.6 mm 5 gels 199-18011
SuperSep Phos-tag™ Precast gels for EasySeparator (Wako's electrophoresis tank)
SuperSep Phos-tag™ (50 μmol/L), 6%, 13 well 5 gels 192-17401 Keep at 2~10℃
SuperSep Phos-tag™ (50 μmol/L), 6%, 17 well 5 gels 199-17391
SuperSep Phos-tag™ (50 μmol/L), 7.5%, 13 well 5 gels 195-17371
SuperSep Phos-tag™ (50 μmol/L), 7.5%, 17 well 5 gels 192-17381
SuperSep Phos-tag™ (50 μmol/L), 10%, 13 well 5 gels 193-16711
SuperSep Phos-tag™ (50 μmol/L), 10%, 17 well 5 gels 190-16721
SuperSep Phos-tag™ (50 μmol/L), 12.5%, 13 well 5 gels 195-16391
SuperSep Phos-tag™ (50 μmol/L), 12.5%, 17 well 5 gels 193-16571
SuperSep Phos-tag™ (50 μmol/L), 15%, 13 well 5 gels 193-16691
SuperSep Phos-tag™ (50 μmol/L), 15%, 17 well 5 gels 196-16701
SuperSep Phos-tag™ (50 μmol/L), 17.5%, 13 well 5 gels 197-16851
SuperSep Phos-tag™ (50 μmol/L), 17.5%, 17 well 5 gels 194-16861
Related Products
α-Casein, from Bovine milk, DephosphorylatediMixture of Phosphorylated α-Casein and Dephosphorylated α-Casein) 1 mgs 038-23221 Keep at 2~10℃
10 mgs 034-23223
EasySeparator (an electrophoresis tank for SuperSep) 1 unit 058-07681 RT
Wide-View Prestained Protein Size Marker Ⅲ (11~245 kDa) 500 μL
(for 200 tests)
230-02461 Keep at -20℃