Phos-tag™ PAGE GUIDEBOOK

Related ProductsiElectrophoresis/Western Blottingj

Reagents for Phos-tag™ SDS-PAGE gel preparation

Product Name Pkg. Size Wako Cat. No. Use Application
30 w/v% Acrylamide solution, 29:1 500 mL 014-21705 Ready-to-use gSolution Ah. 30%T, 3.3%C
Agarose H (High-strength type) 1 g 315-01203 High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 - 1% and a separation range of 1 - 200 kbp.
10 g 319-01201
25 g 317-01202
10% SDS Solution 100 mL 311-90271 Ready-to-Use gSolution Dh
500 mL 313-90275
Manganese(Ⅱ) Chloride Tetrahydrate, 99.0+ % (Titration) 25 g 134-15302 for Molecular Biology Please use for preparation of gSolution Fh
100 g 136-15301
Zinc Chloride 25 g 268-01902 for Molecular Biology
Separating Gel Buffer Solution (x4) 250 mL 192-11041 Ready-to-Use mixed solution of gSol. Bh and gSol. Dhfor preparation of Resolving Gel. Contains SDS.
Stacking Gel Buffer Solution (x4) 250 mL 199-11051 Ready-to-Use mixed solution of gSol.Ch and gSol. Dhfor preparation of Stacking Gel. Contains SDS.
N,N,N',N'-Tetramethylethylenediamine (TEMED) 25 mL 205-06313 for Electrophoresis
10 w/v% Ammonium Peroxodisulfate Solution (Ammonium Persulfate Solution) 25 mL 019-15922 Ready-to-Use gSolution Gh

Premixed Buffers

Product Name Pkg. Size Wako Cat. No. Use Application
Running Buffer Solution (x10) 1 L 184-01291 Ready-to-Use concentrated gSolution Hh
SDS-PAGE 10x Running Buffer 1 L 312-90321 Ready-to-Use concentrated gSolution Hh
5 L 318-90323
SDS-PAGE Buffer, pH 8.5 5 L 192-16801 Ready-to-Use gSolution Hh, 1 x buffer.
Tricine Running Buffer Solution (x10) 1 L 200-17071 Composition: 0.5 M Tris / 0.5 M Tricine / 1% SDS
Sample Buffer Solution (2ME+) (x4) 25 mL 191-13272 Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol
Sample Buffer Solution (2ME+) (x2) 25 mL 196-11022
Sample Buffer Solution
with 3-Mercapto-1,2-propanediol (x2)
25 mL 199-16132 Laemmli Sample Buffer containing 3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME
Sample Buffer Solution
with 3-Mercapto-1,2-propanediol (x2)
25 mL 196-16142

Reagents for Staining

Product Name Pkg. Size Wako Cat. No. Use Application
Quick CBB Plus 250 mL 174-00553 Ready-to-Use Sol. K.Fixing and destaining procedure are not required.No organic solvents are necessary.Protein bands are stained in 10 minutes.
1 L 178-00551
Quick-CBB
EStaining solution A: 1L ~ 1
EStaining solution B: 1L ~ 1
2 L 299-50101 By mixing staing solution A and B, ready-to-Use Sol. K
Silver Stain MS Kit 20 tests 299-58901 Proteins are rarely modified chemically due to omitting treatment of glutaraldehyde and is detected at sub-nanogram level on the elecrophoretic gel.
Silver Stain Kit Wako for10 gels 299-13841 50~100 times more sensitive than CBB method.
Silver Stain II Kit Wako for10 gels 291-50301 This kit contains Stopper, which can be adjusted the staining strength.
Negative Gel Stain MS Kit 20 tests 293-57701 Applicable for mass analysis and western blot

Protein Size Marker

Product Name Pkg. Size Wako Cat. No. Use Application
WIDE-VIEW™ Prestained Protein Size Marker Ⅲ 500 μL 230-02461 A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less to distort.

Enzyme for Dephosphorylation

Product Name Pkg. Size Wako Cat. No. Use Application
Alkaline Phosphatase ifor Biochemistryj 50 U 018-10693 Applicable for dephosphorylation of proteins
100 U 012-10691

Electrophoresis ApparatusEPrecast Gels

Product Name Pkg. Size Wako Cat. No. Use Application
EasySeparator 1 set 058-07681 An electrophoresis tank for SuperSep precast polyacrylamide gels.
SuperSep Ace, 12.5%, 13 well 10 gels 199-14971 Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation. Expire in 9 months after the manufacture
SuperSep Ace, 12.5%, 17 well 10 gels 196-14981
SuperSep Ace, 15%, 13 well 10 gels 193-14991
SuperSep Ace, 15%, 17 well 10 gels 190-15001

FInvitrogenfs electrophoresis tank is also applicable to SuperSep by using an adjuster. Please contact us.

Reagents for Western Blotting

Product Name Pkg. Size Wako Cat. No. Use Application
ImmunoStar LD
ELuminescence solution A
ELuminescence solution B
200 ㎠ 296-69901 Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as substrate. Not available for sale in the US and Europe.
1,000 ㎠ 292-69903
2,000 ㎠ 290-69904
ImmunoStar Zeta 200 ㎠ 291-72401 Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal.
1,000 ㎠ 297-72403
2,000 ㎠ 295-72404
Immuno Enhancer 2 assays 294-68601 Ready-to-Use Immunoreaction Enhancer for western blotting and ELISA
10 assays 290-68603
40 assays 298-68604

FNot available for sales in the US and Europe.

e-Reagent