Phos-tag™ is …
- 【Basic Structure of Phos-tag™】
- M2+：Zinc ion or manganese ion
- ◇ Selectivity of binding of a phosphate ion (2-) is much higher than that of other anions.
- ◇ Stable complex is formed under physiological conditions (pH 5 to 8).
|Product||Purpose of Use|
|Phos-tag™ Acrylamide||Separation：Separation is possible by SDS-PAGE depending on the degree of phosphorylation.|
|SuperSep Phos-tag™||Separation：Ready-to-use precast gel containing 50 µM Phos-tag™ Acrylamide|
|Phos-tag™ Biotin||Detection：A substitute for the anti-phospho antibody used in western blot.|
|Phos-tag™ Agarose||Purification：Phosphorylated proteins are purified by column chromatography.|
|Phos-tag™ Tip||Purification：Ready-to-use tip to purify phosphorylated peptides|
Mass Analytical Kit
|Analysis：This is used in MALDI-TOF/MS analysis to improve the detection sensitivity of phosphorylated molecules.|
Phos-tag™ was developed by the Department of Functional Molecular Science at Hiroshima University. http://www.phos-tag.com/
Two metallic ions cooperate to bind a phosphate group
1. Divalent metal ions trap phosphorylated proteins during migration.
2. The higher the amount of phosphorylation, the slower the migration velocity.
3. Separation occurs based on phosphorylation levels.
(Separation can occur if the phosphorylation sites are different, even with identical levels of phosphorylation)
Application - Time-course of α-casein dephosphorylation -