Phos-tag™ PAGE GUIDEBOOK

SuperSep Phos-tag™

SuperSep Phos-tag™ is a precast gel, which can be immediately used after opening the package. It contains zinc as a supplemental metal. It has excellent storage stability by its neutral gel buffer and ZnCL2. Sharp bands can be obtained.

SuperSep Phos-tagTM

Features

● Ready-to-use

● Safety due to precast gel

● Long-term stability (Stable for 9 months)

● Almost the same basic mechanism as normal SDS-PAGE

● High reproducibility

Plate Size 100 x 100 x 3 (mm) ZnCl2 conc. 100 µmol/L
Gel Size 90 x 85 x 1 (mm) Well volume 30 µL 25 µL
Well number 13 17 Storage Condition Keep at 2〜10℃
Phos-tag™ conc. 50 μmol/L
ATTENTION

・Use of a normal prestained marker will distort the bands. Use of WIDE-VIEW™ Prestained Protein Size Marker III
(Wako Cat. No. 230-02461) is recommended. Please refer to “Phos-tag™ Acrylamide - Molecular Marker”
in “FAQ.”

・Before using this product, check a sample for migration pattern problems with “SuperSep Ace” or other normal SDS-PAGE.

・When performing Western blotting, execute an EDTA process before transfer. For details, refer to “Troubleshooting.”

Application ~ Dephosphorylation over time of β-casein ~

SuperSep Ace, 12.5%, 13 wells/SuperSep Phos-tag™, 12.5%, 13 wells

[ Buffer ] Tris-Glycine-SDS electrophoresis buffer

[ Sample ] M: WIDE-VIEW™ Prestained Protein Size Marker Ⅲ (Wako Cat.No. 230-02461)

1 : 0 min. β-casein (with AP treatment)

2 : 15 min. β-casein (with AP treatment)

3 : 30 min. β-casein (with AP treatment)

4 : 45 min.β-casein (with AP treatment)

5 : 60 min. β-casein (with AP treatment)

[ Condition ] Constant current 35 mA for 60 min.

[ Staining ] Quick-CBB Staining(Wako Cat. No. 299-50101)

[ Destaining ] Deionized water with microwave

β-casein was dephosphorylated over time. Dephosphorylated β-casein can be separated from β-casein with SuperSep Phos-tag™.

Product List

(1)for Bio-Rad's Electrophoresis Tank NEW

Product Name Pkg. Size Wako Cat. No. Electrophoresis Tank
SuperSep Phos-tag™ (50 µmol/L), 7.5%, 17 wells 83 x 100 x 3.9 mm 5 gels 198-17981 Mini-PROTEAN® Tetra Cell
(Bio-Rad Laboratories, Inc.)
SuperSep Phos-tag™ (50 μmol/L), 12.5%, 17 wells 83 x 100 x 3.9 mm 195-17991

Mini-PROTEAN is a registered trademark of Bio-Rad Laboratories, Inc.

(2)for Life Technologies' Electrophoresis Tank NEW

Product Name Pkg. Size Wako Cat. No. Electrophoresis Tank
SuperSep Phos-tag™ (50 µmol/L), 7.5%, 17 wells 100 x 100 x 6.6 mm 5 gels 192-18001 XCell SureLock™ Mini-Cell
(Life Technologies, Inc)
SuperSep Phos-tag™ (50 µmol/L), 12.5%, 17 wells 100 x 100 x 6.6 mm 199-18011

XCell SureLock is a registered trademark of Life Technologies, Inc.

(3)for Wako’s Electrophoresis Tank

Product Name Wako Cat. No. Pkg. Size Electrophoresis Tank
SuperSep Phos-tag™ (50 µmol/L), 6%, 13 wells 192-17401 5 gels EasySeparator
EasySeparator
SuperSep Phos-tag™ (50 µmol/L), 6%, 17 wells 199-17391
SuperSep Phos-tag™ (50 µmol/L), 7.5%, 13 wells 195-17371
SuperSep Phos-tag™ (50 µmol/L), 7.5%, 17 wells 192-17381
SuperSep Phos-tag™ (50 µmol/L), 10%, 13 wells 193-16711
SuperSep Phos-tag™ (50 µmol/L), 10%, 17 wells 190-16721
SuperSep Phos-tag™ (50 µmol/L), 12.5%, 13 wells 195-16391
SuperSep Phos-tag™ (50 µmol/L), 12.5%, 17 wells 193-16571
SuperSep Phos-tag™ (50 µmol/L), 15%, 13 wells 193-16691
SuperSep Phos-tag™ (50 µmol/L), 15%, 17 wells 196-16701
These SuperSep Phos-tag™ precast gels are designed for the ʻEasySeparatorʼ tank.
EasySeparator™ 058-07681 1 unit