Phos-tag™ PAGE GUIDEBOOK

Phos-tag™ Series

Product Name Pkg. Size Wako Cat. No. Maker’s code No. Applications
Phos-tag™ Biotin BTL-111
1mM Aqueous Solution Ref.1)
0.1 mL 308-97201 BTL-111S1 BTL-111 offers higher sensitivity than BTL-104.
Phos-tag™ Biotin BTL-104 10 mg 301-93531 BTL-104 Specific detection without any anti-phosphorylated antibodies on Western blot. Detection is possible regardless of the type of phosphorylated amino acid.
Phos-tag™ Mass Analytical Kit 1 Kit 305-93551 MS-101KIT Analysis by MALDI-TOF/Mass
Phos-tag™ Agarose 0.5 mL 302-93561 AG-501 Enrichment, separation and purification of phorphorylated proteins using column chromatography
3 mL 308-93563 AG-503
Phos-tag™ Tip 8tips 387-07321 AG2-103

Phos-tag™ Biotin - Detection of phosphoprotein by Western blotting -

This biotin-bound Phos-tag™ is used for the detection of phosphoprotein by Western blotting.

Features

● Recommended when anti-phosphorylated antibodies are not available regardless of the type of phosphorylation.

● No special reagent or device is necessary.

It can be conveniently used even if target
anti-phosphorylated Thr/Ser antibody is not available !

※BTL-104, and BTL-111 have linkers with different lengths. BTL-111 offers higher sensitivity.

Schematic of western blotting using Phos-tag™ Biotin

Schematic of western blotting using Phos-tag™ BiotinPhosphorylated proteins on PVDF membrane can be detected similarly to the anti-phosphorylation antibody in Western blotting.

BTL-104 M.W. 767 BTL-111 M.W. 1,367

  • Application

  • 〜Detection of β-casein, Ovalbumin〜

〜Detection of β-casein, Ovalbumin〜

NARD institude,ltd.

Phos-tag™ Mass Analytical Kit - Detection Sensitivity of MALDI-TOF/Mass is improved -

Before use, Phos-tag™ Mass Analytical Kit is mixed with samples for MALDI-TOF/Mass analysis. Phosphorylated molecule-Phos-tag™ complex is detected in a positive mode, and phosphorylated molecules usually difficult to detect can be detected with improved sensitivity.Three types of reagents contain different types of zinc.

Features

● Detection sensitivity of phosphorylated molecule is improved.

● Non-phosphorylated molecules are not detected.

● Applicable to phosphorylated molecules other than phosphorylated peptides.

【Kit Contents】
Phos-tag™ MS-101L ……5mℊ
 ([C27H29N6O64Zn2]3+ MW:581.4)
Phos-tag™ MS-101H ……5mℊ
 ([C27H29N6O68Zn2]3+ MW:589.4)
Phos-tag™ MS-101N ……10mℊ
 ([C27H29N6OZn2]3+ MW:584.3)

The detection sensitivity of phosphorylated LPA is improved.

  • Application

  • 〜Detection of Phos-tag™
    -phosphorylated LPA complex〜

〜Detection of Phos-tag™-phosphorylated LPA complex〜

Phos-tag™ Agarose
- Purification of phosphorylated proteins by affinity chromatography -

MANAC incorporated

Fill Phos-tag™ Agarose in a column for use. It can be used for separation, purification and concentration of phosphorylated proteins. Because it is free of surfactants and reducing agents, phosphorylated proteins can be obtained in a condition similar to in vivo.

Features

● Phosphorylated proteins can be purified within 1 hour.

● The proteins can be trapped
in physiological condition (pH 7.5).

● Purified without reducing agent and surfactant.

Phos-tag™ Agarose

  • Application

  • 〜Purification of phosphorylated proteins in A431 lysate〜

Schematic of western blotting using Phos-tag™ Biotin

Lysate was applied to a column filled with Phos-tag™ Agarose.Phosphorylated proteins were concentrated in the adsorbed fraction.

Lysate was applied to a column filled with Phos-tag™ Agarose.
Phosphorylated proteins were concentrated in the adsorbed fraction.

  • M:Molecular Marker
  • Lane 1:Non adsorbed fraction
  • Lane 2:Adsorbed fraction
  • Lane 3:Column rinsing fraction
  • (Left) Fluorescence staining
  • (right) Western blotting with anti-phosphorylated Tyr

NARD institude,ltd.

Phos-tag™ Tip
- Ready-to-use Phos-tag™ tip for the concentration of phosphorylated peptides -

Features

● The operation time is less than 30 minutes.

● High recovery rate

● No expensive device required

Phos-tag™ Tip

  • Application

  • 〜Isolation of substances digested with 6 nmol β-casein trypsin〜

〜Isolation of substances digested with 6 nmol β-casein trypsin〜