Phos-tag™ PAGE GUIDEBOOK

Related Products(Electrophoresis/Western Blotting)

Reagents for Phos-tag™ SDS-PAGE gel preparation

Product Name Pkg. Size Wako Cat. No. Use Application
30 w/v% Acrylamide / Bis Mixed solution(29:1) 500 mL 015-25635 Ready-to-use “Solution A”. 30%T, 3.3%C
Agarose H(High-strength type) 1 g 315-01203 High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 - 1% and a separation range of 1 – 200 kbp.
10 g 319-01201
25 g 317-01202
10% SDS Solution 100 mL 311-90271 Ready-to-Use “Solution D
500 mL 313-90275
Manganese(Ⅱ) Chloride Tetrahydrate,
99.0+ %(Titration)
25 g 134-15302 for Molecular Biology
Please use for preparation of “Solution F
100 g 136-15301
Zinc Chloride 25 g 268-01902 for Molecular Biology. Please use for preparation of “Solution M
Bis-Tris 100 g 345-04741 Please use for preparation of “Solution N
Sodium Hydrogensulfite 〈JIS Special Grade〉 25 g 196-01372 Please use for preparation of “Solution O
100 g 198-01371
Sodium Hydrogensulfite
〈For Molecular Biology〉
100 g 190-16461
MOPS 100 g 345-01804 Please use for preparation of “Solution P
250 g 341-01801
MOPS 〈For Molecular Biology〉 100 g 341-08241
500 g 343-08245
Separating Gel Buffer Solution (x4) 250 mL 192-11041 Ready-to-Use mixed solution of “Sol. B” and “Sol. D”for preparation of Resolving Gel. Contains SDS.
Stacking Gel Buffer Solution (x4) 250 mL 199-11051 Ready-to-Use mixed solution of “Sol.C” and “Sol. D”for preparation of Stacking Gel. Contains SDS.
N,N,N',N'-Tetramethylethylenediamine (TEMED) 25 mL 205-06313 for Electrophoresis
10 w/v% Ammonium Peroxodisulfate Solution
(Ammonium Persulfate Solution)
25 mL 019-15922 Ready-to-Use “Solution G

Premixed Buffers

Product Name Pkg. Size Wako Cat. No. Use Application
Running Buffer Solution (x10) 1 L 184-01291 Ready-to-Use concentrated “Solution H
SDS-PAGE 10x Running Buffer 1 L 312-90321 Ready-to-Use concentrated “Solution H
5 L 318-90323
SDS-PAGE Buffer, pH 8.5 5 L 192-16801 Ready-to-Use “Solution H”, 1 x buffer.
Tricine Running Buffer Solution (x10) 1 L 200-17071 Composition: 0.5 M Tris / 0.5 M Tricine / 1% SDS
Sample Buffer Solution (2ME+) (x4) 25 mL 191-13272 Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol
Sample Buffer Solution (2ME+) (x2) 25 mL 196-11022
Sample Buffer Solution
with 3-Mercapto-1,2-propanediol (x2)
25 mL 199-16132 Laemmli Sample Buffer containing 3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME
Sample Buffer Solution
with 3-Mercapto-1,2-propanediol (x2)
25 mL 196-16142
AquaBlot™ 10x Tris-Glycine-SDS Transfer
Buffer
1 L 019-25111 Please use it to transfer to a membrane. Containing 0.05% SDS

Enzyme for Dephosphorylation

Product Name Pkg. Size Wako Cat. No. Use Application
Alkaline Phosphatase(for Biochemistry) 50 U 018-10693 Applicable to dephosphorylation of proteins
100 U 012-10691

Reagents for Staining

Product Name Pkg. Size Wako Cat. No. Use Application
Quick CBB Plus 250 mL 174-00553 Ready-to-Use Sol. K.
Fixing and destaining procedure are not required.
No organic solvents are necessary.
Protein bands are stained in 10 minutes.
1 L 178-00551
Quick-CBB
・Staining solution A: 1L x 1
・Staining solution B: 1L x 1
2 L 299-50101 By mixing staing solution A and B, ready-to-Use Sol. K
Silver Stain MS Kit 20 tests 299-58901 Modified proteins by glycosilation and / or phosphorylation can be detected sub-nanogram level on the electrophoretic gel.
Silver Stain Kit Wako for10 gels 299-13841 50~100 times more sensitive than CBB method.
Silver Stain Ⅱ Kit Wako for10 gels 291-50301 This kit contains Stopper, which can be adjusted the staining strength.
Negative Gel Stain MS Kit 20 tests 293-57701 Applicable to mass analysis and Western blot

Protein Size Marker

Product Name Pkg. Size Wako Cat. No. Use Application
WIDE-VIEW™ Prestained Protein Size Marker Ⅲ 25 μL 236-02463 A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less distorted.
500 μL 230-02461
500 μL x 3 234-02464

Positive Control(for confirmation of separation capacity of Phos-tag™ SDS-PAGE)

Product Name Pkg. Size Wako Cat. No. Use Application
α-Casein, from BovineMilk, Dephosphorylated 1 mg 038-23221 Mixture of phosphylated and dephosphorylated α-casein
10 mg 034-23223

Electrophoresis Apparatus ・ Precast Gels

Product Name Pkg. Size Wako Cat. No. Use Application
EasySeparator 1 unit 058-07681 An electrophoresis tank for SuperSep precast polyacrylamide gels.
SuperSep Ace, 6%, 13 wells 10 gels 195-15171 Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation.
Expired in 9 months after manufacture
SuperSep Ace, 7.5%, 13 wells 10 gels 198-14941
SuperSep Ace, 7.5%, 17 wells 10 gels 191-14931
SuperSep Ace, 10%, 13 wells 10 gels 195-14951
SuperSep Ace, 10%, 17 wells 10 gels 192-14961
SuperSep Ace, 12.5%, 13 wells 10 gels 199-14971
SuperSep Ace, 12.5%, 17 wells 10 gels 196-14981
SuperSep Ace, 15%, 13 wells 10 gels 193-14991
SuperSep Ace, 15%, 17 wells 10 gels 190-15001

Reagents for Western Blotting

Product Name Pkg. Size Wako Cat. No. Use Application
ImmunoStar LD
・Luminescence solution A
・Luminescence solution B
200 cm2 296-69901 Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate.
Not available for sale in the US and Europe.
1,000 cm2 292-69903
2,000 cm2 290-69904
ImmunoStar Zeta 200 cm2 291-72401 Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal.
1,000 cm2 297-72403
2,000 cm2 295-72404
ImmunoStar Basic 200 cm2 295-75101 A cost-effective, stable and long-lasting product.
2,000 cm2 291-75103
5,000 cm2 299-75104
Immuno Enhancer 2 assays 294-68601 Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA
10 assays 290-68603
40 assays 298-68604

* : Not available for sales in the US and Europe.

e-Reagent