A. Inhibitors

Novel Inhibitors

Wako commercialized new inhibitors which were discovered by Dr. Hiroyuki Osada, Antibiotics laboratory of Institute of Physical and Chemical Research (RIKEN) under license from RIKEN.

HSP60 Inhibitor
ETB

[Reference]
Nagumo, Y., Kakeya, H., Shoji, M., Hayashi, Y., Dohmae, N. and Osada, H.: Biochem. J.: 387(3), 835 (2005).
Th is product is a derivative of epolactaene isolated from Penicillium. It has a more potent cytostatic eff ect on human neuroblastoma cells SH-SY 5Y than that of epolactaene, and induces apoptosis. Furthermore, it has been revealed that ETB induces apoptosis in human T-lymphoma cells Jurkat. Recently, HSP60 was identifi ed as one of ETB binding proteins. ETB binds to HSP60 to inhibit chaperone activity.
Protein Synthesis Inhibitor
Reveromycin A Sodium Salt

[Reference]
Woo, J. T., Kawatani, M., Kato, M., Shinki, T., Yonezawa, T., Kanoh, N., Nakagawa, H., Takami, M., Lee, K.H., Stern, P.H., Nagai, K. and Osada, H.: Proc. Natl. Acad.Sci. USA: 103(12), 4729 (2006).
Th is product is an antibiotic isolated from Streptomyces. It targets isoleucyl-tRNA synthetase and inhibits protein synthesis in eukaryotes. It has been investigated for its antitumor and antifungal activities. However, recent studies have revealed that low-dose of reveromycin A induces cell death of activated osteoclasts, which leads to acidic environment. Th us it receives attention as a candidate for the treatment of osteoporosis/ multiple myeloma.
(Th is product is a sodium salt of the compound shown right.)
Apoptosis Inhibitor
RKTS-33

[Reference]
Mitsui, T., Miyake, Y., Kakeya, H., Hayasi, Y., Osada, H. and Kataoka, T.: Biosci. Biotechnol. Biochem.: 69(10), 1923 (2005).
Th is product is a derivative of epoxycylohexenone isolated from Paecilomyces. It has lower toxicity than epoxycylohexenone. Like epoxycylohexenone, it inhibits apoptosis not by inhibition of perforin-dependent pathway by cytotoxic T lymphocytes but by selective inhibition of Fas ligand-dependent pathway alone.
Cell Cycle Inhibitor
Tryprostatin A

[Reference]
Usui, T., Kondoh, M., Cui, C.B., Mayumi, T. and Osada, H.: Biochem. J.: 333(3), 543 (1998).
Th is product is an alkaloid antibiotic isolated from Aspergillus. It aff ects the microtubule-associated protein binding site and exhibits antitumor activity by inhibition of cell cycle progression in the M phase specifi cally
Description Wako Cat. No. (Pkg. Size) Grade Storage
GSP60 Inhibitor ETB 051-07671 (200 μL) for Cellbiology Ship and keep at -20°C
Protein Synthesis Inhibitor Reveromycin A Sodium Salt 185-02181 (500 μg)
Apoptosis Inhibitor RKTS-33 182-02191 (200 μg)
Cell Cycle Inhibitor Tryprostatin A 203-16961 (500 μg)
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Wako Product Update No.19 [ page. 3 ]