D. Cell Culture

1. Recombinant Protein expressed in PLANTS containing No Animal-derived Components

Culture media without animal by-products have been attracting attention in tissue cultures for regenerative medicine and in the manufacturing of vaccines or antibody drugs with cultured animal cells, etc.
In order to respond to these needs, Wako provides four kinds of recombinant proteins expressed in plants, namely bovine aprotinin, human lactoferrin, human lysozyme and bovine trypsin. Th ese products are free from contamination of viruses of human origin or BSE causative substances.
Aprotinin, Bovine, recombinant, expressed in Plants, Solution
Th is product is used for the inactivation of trypsin by adding it after trypsinization of adherent cultured animal cells.

Protease inhibitory activity: > 5TIU/mg protein
1 TIU (Trypsin Inhibitor Unit)≒ 900 KIU (Kallikrein Inhibitor Unit)
8 UIP (Peptidases Inhibitor Units) = 1 KIU (Kallikrein Inhibitor Unit)

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Lactoferrin, Human, recombinant, expressed in Plants
This product is used suitably for the cultivation of general cells or cells with low growth effi ciency, and for the production of IgG1 from hybridomas. IgG1 production from hybridomas in the serum-free minimal medium is about 1.5 times higher than in the transferrin-added media.
Protease inhibitory activity: > 5TIU/mg protein
1 TIU (Trypsin Inhibitor Unit)≒ 900 KIU (Kallikrein Inhibitor Unit)
8 UIP (Peptidases Inhibitor Units) = 1 KIU (Kallikrein Inhibitor Unit)

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Lysozyme, Human, recombinant, expressed in Plants
This product exhibits over 4 times higher bacteriolytic activity against Gram positive and negative bacteria when compared to Lysozyme from chicken egg white. The bacteriolytic activity is comparable with Lysozyme of human origin. It is used optimally for the prevention of contamination when producing recombinant proteins from animal cells.
Unit definition: One unit is defined as the amount of enzyme that will cause a change in absorbance of 0.001 at 450 nm using a suspension of Micrococcus bacteria as substrate.

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Trypsin, Bovine, recombinant, expressed in Corn
This product is used for the trypsinization of adherent cultured animal cells.
Activity: ≥ 72 TAME units/mg (≥ 3,300 USP units/mg)
Unit definition: One TAME unit is defi ned as the amount of enzyme that will catalyze the hydrolysis of 1 μmol Na-p-tosyl-Larginine methyl ester (TAME) per minute in the presence of 0.01mol/L calcium ion at 25 °C, pH 8.2.
1 TAME unit = 19.2 USP unit = 57.5 BAEE unit
Description Wako Cat. No. (Pkg. Size) Grade Storage
Aprotinin, Bovine, recombinant expressed in plants, Solution 014-21021 (500 μL) for Cell Culture Ship and keep at 2-10 ˚C
010-21023 (1,000 μL)
Lactoferrin, Human, recombinant expressed in plants [rhLF] 188-02051 (50 mg)
184-02053 (100 mg)
182-02054 (500 mg)
Lysozyme, Human, recombinant expressed in plants 185-02061 (10 mg) Ship and keep at -20 ˚C
181-02063 (100 mg)
189-02064 (500 mg)
Trypsin, Bovine, recombinant expressed in corn 208-15931 (5 mg) for Cellbiology
204-15933 (50 mg)
Albumin, Human, recombinant expressed in plants
018-21541 (1 g) for Cell Culture
014-21543 (5 g)
016-21542 (25 g)
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Wako Product Update No.19 [ page. 7 ]