G. Epigenetics

LabAssay™ is an ELISA kit for simultaneous measurement of multi animal-samples using a microplate as well as measurement using test tubes.

1. DNA Methylation

Detection Wako Cat. No. (Pkg. Size)  

(1) DNA Methylation Inhibitors

Methylation of DNA contributes to the epigenetic regulation of gene expression. Methylated DNAs are formed by addition of methyl group to the carbon atom at 5-position of C (cytosine) bases in DNA by DNA methyltransferase (DNMT).
Two kinds of DNA methylation by DNMT are observed; one is called maintenance DNA methylation by DNMT1 (methylation is maintained after DNA replication), and the other is de novo DNA methylation by DNMT3a and DNMT3b (new methylation occurs in unmethylated DNA). Since the amount of methylated DNA changes depending on the types of cancer and germ cells or cell cycle, gene expression can be analyzed in detail by effective inhibition of DNA methylation.

5-Azacytidine, 98.0+% (HPLC)
C8H12N4O5 = 244.20, CAS No. 320-67-2

for Biochemistry, Keep and ship at -20 °C

This product is one of the DNA methylation inhibitors that induce demethylation of genomic DNA in a concentration-dependent way 1). It is used for researches on methylation of the promoter site in prostatic cancer cells 2).
016-16711 (50 mg)
012-16713 (250 mg)
010-16714 (1 g)
image [References]
1) Stresemann, C., et al.: Cancer Res., 66, 2794 (2006).
2) Leiblich, A., et al.: Oncogene., 25, 2953 (2006).
3) Bowers R.R., et al.: PNAS., 103, 13022 (2006).
4) Cheng C., et al.: Mol. Genet. Genomics, 276, 378 (2006).

5-Aza-2'-deoxycytidine, 98.0+% (HPLC)
C8H12N4O4 = 228.21, CAS No. 2353-33-5

for Genetic Research, Keep and ship at -20°C

This product is one of the cytidine analog DNA methylation inhibitors that induce demethylation of genomic DNA in a concentration-dependent way 1). It is used for researches on methylation of the promoter site in lung cancer cells 2).
018-20941 (10 mg)
014-20943 (50 mg)
image [References]
1) Stresemann, C., et al.: Cancer Res., 66, 2794 (2006).
2) Vuillemenot, B.R., et al.:Mol. Cancer. Res., 4, 267 (2006)
3) Kim T.Y., et al.: Cancer Res., 66, 7490 (2006).
4) Fulda S., et al.: Oncogene, 25, 5125 (2006).

DNA Methyltransferase Inhibitor, 98.0+% (HPLC)
C19H14N2O4 = 334.33

for Genetic Research, Keep and Ship at -20°C

This product inactivates DNA methyltransferase activity by binding to its active center unlike 5-azacytidine or 5-aza-2'-deoxycytidine.
041-30101 (10 mg) 047-30103 (25 mg) image [References]
1) Stresemann, C., et al.: Cancer Res., 65, 6305 (2005).

(-)-Epigallocatechin Gallate, 90.0+% (HPLC)

for Biochemistry, Keep at 2~10°C and ship at RT
C22H18O11 = 458.37, CAS No. 989-51-5
059-05411 (100 mg) Polyphenol:
  Catechin from green tea extracts
Solubility:
  Soluble in methanol (10 g/L methanol soln.)

Procaine Hydrochoride, 99.0+% (Titration)

for Biochemistry, Keep and ship at RT
C13H20N2O2・HCl = 272.77, CAS No. 51-05-8
167-15111 (50 g) Solubility:
  Freely soluble in water, soluble in
  ethanol and sparklingly soluble in
  chloroform

Zebularine, 97.0+% (HPLC)
C19H12N2O5 = 228.20

for Genetic Research, Keep and ship and 4°C

This product is one of the cytidine analog DNA methylation inhibitors that induce demethylation of genomic DNA in a concentration-dependent way 1). It is known to be highly stable in solution and to have low toxicity 2).
267-01891 (5 mg)
263-01893 (25 mg)
image [References]
1) Stresemann, C., et al.: Cancer Res., 66, 2794 (2006).
2) Cheng, J.C., et al.: J. Natl. Cancer Inst., 95, 399 (2003).
3) Dote H., et al.: Clin. Cancer Res., 11, 4571 (2005).
4) Hodge D.R., et al.:. Cancer Res., 65, 4673 (2005).

(2) Detection of Cell Proliferation

5-Bromo-2'-deoxyuridine, [5-BrdU], 98.0+% (HPLC)

for Biochemistry, Keep and ship at -20°C
C9H11BrN2O5 = 307.10, CAS No. 59-14-3
027-15561 (1 g)
023-15563 (5 g)
 

(3) Modified Nucleotides

Modified nucleotide triphosphates for epigenetics research, which are applicalbe to PCR, are available.

2'-Deoxyinosine 5'-Triphophate Solution [dITP Solution], 98+% (HPLC)

for Molecular Biology, Keep and ship at -20°C

CAS No. 95648-77-4

048-29861
(25 μmol (100 mM))
pH: approximately 7

5-Hydroxymethyl-2'-deoxycytidine 5'-Triphosphate Soln. [HMdCTP Solution], 98+%

for Genetic Research, Keep and ship at -20°C
083-08371
(25 μmol (100 mM))
pH: approximately 7

5-Methyl-2'-deoxycytidine 5'-Triphosphate Soln. [dm5CTP Solution], 95+% (HPLC)

for Genetic Research, Keep and ship at -20°C

CAS No. 838-07-3

049-29891
(1 μmol (10 mM))
 

N4-Methyl-2'-deoxycytidine 5'-Triphosphate Solution. [dm4CTP Solution], 95+% (HPLC)

for Genetic Research, Keep and ship at -20°C
042-29881
(1 μmol (10 mM))
 

N6-Methyl-2'-deoxycytidine 5'-Triphosphate Solution. [dm4CTP Solution], 95+% (HPLC)

for Genetic Research, Keep and ship at -20°C
045-29871
(1 μmol (10 mM))
 

(4) Others

Anti 5-Methylcytosine, Monoclonal Antibody

for Immunochemistry, Keep and ship at -20°C
015-19721 (100 μL) Mouse ascites filtrate, Subclass: IgM,
Specific to methylcytosine, and reactive with cytosine and thymidine than 1%.
Working dilution: Westernblot (1:1,000~1:10,000); Immunofluorescence (1:100)

Micrococcal Nuclease, lyophilized

for Genetic Research, Keep and ship at 2~10°C

CAS No. 9013-53-0, EC No. 3.1.31.1,
MW: 168,000

137-14731 (abt.15,000 units/vial) Application: Nuclesome modeling experimentation

2. Cell Cycle and Transcription

Detection Wako Cat. No. (Pkg. Size)  

(1) Histone Deacetylase (HDAC) Inhibitors

HDAC plays a central role in chromatin structure formation associated with the nuclear distribution of DNA. HDAC inhibitors show connections with cell division cycles and differentiation, as well as with antitumor activity and apoptosis-inducing activity through the inhibition of the deacetylating activity of HDAC.

ITSA1 [N-(1H-Benzotriazol-1-yl)-2,4-dichlorobenzamide]

for Cellbiology, Keep and ship at 2~10°C

C13H7Cl2N3O = 292.12
Inhibitor of Trichostatin A

093-05251 (5 mg)

M 344 [4-Dimethylamino-N-(6-hydroxycarbamoyl-hexyl)-benzamide]

for Cellbiology, Keep and ship at -20°C

C16H25N3O3 = 307.39, CAS No. 251456-60-7
Inhibitor of HDAC Class I and II.
Structural homologue of Trichostatin A.

139-14671 (1 mg)

MC 1293 [3-(4-Toluoyl-1-methyl-1H-2-pyrrolyl)-N-hydroxy-2-propenamide]

for Cellbiology, Keep and ship at -20°C

C16H16N2O3 = 284.31
Inhibitor of HDAC1 and maize deacetylase.

136-14681 (5 mg)

Sirtinol [2-[(2-Hydroxynaphthalen-1-ylmethylene)amino]-N-(1-phenethyl)benzamide]

for Cellbiology, Keep and ship at -20°C

C26H22N2O2 = 394.47
HDAC Class III Inhibitor. Cell-permeable

197-13671 (5 mg)

Splitomicin [1,2-Dihydro-3H-naphtho[2,1-b]-pyran-3-one

for Cellbiology, Keep and ship at 2~10°C

C13H10O2 = 198.22, CAS No. 5690-03-9
HDAC Class III Inhibitor. Cell-permeable

190-13661 (1 mg)

Trichostatin A

for Biochemistry, Keep and ship at -20°C

C17H22N2O3 = 302.37, CAS No. 58880-19-6
Potent & reversible Inhibitor of HDAC Class I & II

200-11993 (1 mg)
204-11991 (5 mg)

Valproic Acid

for Biochemistry, Keep and ship at RT

C8H16O2> = 144.21, CAS No. 99-66-1
Inhibitr of HDAC1

227-01071 (5 g)
225-01072 (25 g)

(2) SIRT1 (classified into HDAC Class III) Activators

Butein [2',4',3,4-Tetrahydroxychalcone],98.0+% (HPLC)

for Biochemistry, Keep and ship at -20°C

C15H12O5 = 272.25, CAS No. 487-52-5
a SIRT1 Activator as well as a protein tyrosine kinase inhibitor

027-14461 (10 mg) Solubility:
Freely soluble in ethanol, soluble in methanol and dimethylsulfoxide. Insoluble in hot water
.
Powder density: 6 mL/g

Piceatannol [3,4,3',5'-Tetrahydroxy-trans-stibene]

for Cellbiology, Keep and ship at 2~10°C

C14H12O4 = 244.24, CAS No. 10083-24-6
a SIRT1 Activator as well as a protein tyrosine kinase syk selective inhibitor

169-21661 (10 mg)

Resveratrol [[trans-3,4',5-Trihydroxystibene],98.0+% (HPLC)

for Biochemistry, Keep and ship at -20°C

C14H12O3 = 228.24
SIRT1 Activator as well as COX-1 inhibitor

185-01721 (100 mg)
181-01723 (500 mg)
Solubility:
Soluble in ethanol and acetone. Insoluble in water.
[Reference] Howitz, K.T., et al.: Nature, 425, 191 (2003).
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Wako Product Update No.19 [ page. 13-15 ]