Food Analysis

1. Presep® Series: Pretreatment Columns

Column for Sample Pretreatment Relating to the Japanese Positive List System

image

Solid-phase extraction, which is used as a sample pretreatment prior to HPLC and GC analysis, has such advantages as easy operation and less solvent usage, and is widely used in many areas. Presep® series are categorized by their shapes; one is "Presep®-C type" which has a cartridge shape with closed both ends, the other is "Presep® syringe type" with one open and one closed ends.

In the Japanese positive list system which came into eff ect in 2006, pretreatment methods using various mini-columns for solid-phase extraction have been adopted. We introduce the Presep® series, which correspond to the fi llers described in "Analytical methods of residual pesticides, feed additives, and veterinary drugs in foods *".

Presep® Series <Filler described in the analytical methods>
Designation described in the analytical methods: * Description Particle size (μm) Weight of Filler Wako Cat. No. (Pkg. Size) Main purpose, characteristics
Octadecylsilylated silica gel mini-column Presep® -C C18 (ODS)(Short) 63 ~ 212 470mg 297-47451 (5 x 10ea.) Reversed-phase partition: Separation of hydrophobic substances in aqueous sample solution
Presep® -C C18 (ODS) 900mg 292-32251 (5 x 10ea.)
Presep® -C C18 (ODS) Type M 5g/25mL 291-48554 (2 x 10ea.);
297-48551 (5 x 10ea.)
Aminopropylsilylated silica gel mini-column Presep® -C NH2 (Short) 38 ~ 63 400mg 299-48751 (5 x 10ea.) Removal of acidic compounds such as organic and fatty acids
Presep® -C NH2 820mg 295-48851 (5 x 10ea.)
Silica gel mini-column Presep® -C Silica Gel 75 ~ 150 800mg 294-31851 (50ea.) Normal-phase absorption: Separation of substances with low to middle polarity in nonaqueous solution
Basic alumina column Presep® -C Alumina 44 ~ 149 1.7g 290-32051 (5 x 10ea.) Removal of interfering substances in pesticides and environmental samples
Synthetic magnesium silicate mini-column Presep® -C Florisil 75 ~ 150 800mg 290-31951 (5 x 10ea.) Removal of lipids, pretreatment of pesticide residue in foods
Presep® Florisil 1g/6mL 291-44051 (5 x 10ea.)
Porous diatomaceous earth column Presep® Diatomaceous Earth, Granular 500 ~1400 1g/6mL 292-35051 (100ea.) Desolvation
2g/15mL 298-35151 (100ea.)
4.5g/25mL 294-35251 (100ea.)
Carboxymethylated weakly acidic cation-exchange resin mini-column Presep® CM 45 ~ 90 250mg/6mL 298-61801 (5 x 10ea.) Polymer based weakly acidic cation-exchange
Presep® Series <Others>
Filler Description Particle size (μm) Weight of Filler Wako Cat. No. (Pkg. Size) Main purpose, characteristics
Agri [Styrene divinylbenzene - Methacrylate based polymer] Presep®-C Agri (Short) 50 220 mg 296-32651 (5 x 10ea.) Collection of high polarity substances, which cannot normally be absorbed on ODS. Pretreatment for residual pesticide analysis.
Presep® Agri 30, 60 500 mg 291-26851 (50ea.)
RPP [Hydrophilic Reversed Phase Polymer] [Styrene divinylbenzene - Polymethacrylate] Presep®-C RPP (Short) 190 mg 297-41851 (5 x 10ea.) Collection of high polarity substances, which cannot normally be absorbed on ODS. Pretreatment of biological samples.
Presep®-C RPP (Long) 360 mg 293-41951 (3 x 10ea.)
Presep® RPP 60 mg/3mL 294-36851 (5 x 10ea.)
200 mg/6mL 290-36951 (5 x 10ea.)
500 mg/6mL 290-37051 (5 x 10ea.)
Glauber's salt, anhydrous (Na2SO4) Presep®-C Na2SO4 45 ~ 90 2.3 g 296-32151 (5 x 10ea.) Dehydration
DEA [Diethylaminoethyl Cl type] Presep® DEA 45 ~ 90 250 mg/6mL 292-61701 (5 x 10ea.) Polymer based weakly basic anion-exchange
QA [Trimethylaminoethyl Cl type] Presep® QA 45 ~ 90 250 mg/6mL 296-61601 (5 x 10ea.) Polymer based strongly basic anion-exchange
S (Sulfonylpropyl Na type] Presep® S 45 ~ 90 250 mg/6mL 294-61901 (5 x 10ea.) Polymer based strongly acidic cation-exchange
Silica gel Presep® Silica Gel Type 3L 20 ~ 40 110 g 292-62801 (5ea.);
298-62803 (30ea.)
Preparative column packed with high quality silica gel and used for fl ash chromatography
Presep® Silica Gel Type M 11 g 297-44151 (2 x 10ea.);
293-44153 (10 x 10ea.)
Presep® Silica Gel Type L 30 g 293-44251 (2 x 10ea.);
299-44253 (10 x 10ea.)
Polyamide resin Presep® Polyamide C-200 Type M 2 g/25mL 296-64901 (5 x 10ea.) Preptreatment of determination of Paeonifl orin, which is component of kakkonto Extract
Silica Gel coated with 2,4-dinitrophenyl Presep®-C DNPH (Short) 75 ~ 150 400 mg 291-43951 (20ea.) Collection of carbonyl compounds in aerial environment and Derivatization with 2,4-dinitrophenyl hydrazone (DNPH)
hydrazine Presep®-C DNPH 0.7 ~ 1 g 290-34251 (20ea.)
Activated Alumina Presep® Alumina, Activated DX 10 g 293-42551 (5ea.)
Active Carbon impregnated Silica Gel Presep® Active Carbon-impregnated Silica Gel (Reverse Column)   1 g 297-43051 (5ea.) Dioxins Analysis
Active Carbon blended Silica Gel Presep® Active Carbon-blended Silica Gel   1 g 299-41551 (10ea.)
Active Carbon impregnated Silica Gel Presep® Active Carbon-impregnated Silica Gel   1 g 293-41451 (10ea.)
multilayer conformed to JIS K0311 and 0312 Presep® Multilayer Silica Gel - 295-41651 (5ea.)
dual stracture column of polypropylene and hydrophobic tefl on membrane-fi lter Presep® Dehydration (30mL) - 293-59901 (50ea.) Separation of water and solvent, which specifi c gravity is light such as ethyl acetate for Organic Synthesis
Presep® Dehydration (48well (3mL)) 294-44351 (10ea.)
Presep® Dehydration 299-40451 (100ea.)
page top
backindexnext
Wako Product Update No.20 [ page. 3 ]