Food Analysis

4. Glycyrrhizin Analysis

Analysis by immunoassay of Glycyrrhizin

Glycyrrhizin (GC) is an active ingredient contained in Glycyrrhiza, which is used for medicinal products and also used as a sweetener in foods.
Glycyrrhizin ELISA Kit Wako is a competitive immunoassay kit with glycyrrhizin-specifi c monoclonal antibody, which was developed under the supervision of Professor Yukihiro Shoyama, Faculty of Pharmaceutical Sciences, Nagasaki International University. Th is kit does not require expensive instruments or organic solvents like the HPLC, which is widely used for the glycyrrhizin analyses, does. Th is kit can be used for analyses of glycyrrhizin in Glycyrrhiza, Glycyrrhiza-containing Chinese medicines, and foods.

image[Standard Curve][Protocol]
image[Standard Curve][Protocol]
Description Wako Cat. No. (Pkg. Size) Storage Shelf Life
Glycyrrhizin ELISA Kit Wako 298-65201 (96 tests) Keep at 2 ~ 10 ˚C 12 months after production
[Kit Contents]
1) GC-Alb Coated Plate1 plate
2) GC Standard Stock Solution200 μL
3) Anti GC Monoclonal Antibody100 μL
4) Enzyme Labeled Secondary Antibody200 μL
5) Reaction Buff er100 mL
6) Wash Stock Solution50 mL
7) Substrate Solution15 mL
8) Stop Solution15 mL
9) Plate Seal2 sheets
Related Products
Description Wako Cat. No.
(Pkg. Size)
Grade Note
Glycyrrhizin, 90.0+% (HPLC)074-03481 (100 mg) for Biochemistry An active component of licorice.
Glycyrrhizic Acid, 99.0+% (HPLC)070-05161 (20 mg) for the Japanese Pharmacopoeia Crude Drugs Test (for Thin-Layer Chromatography) An active component of licorice. Used as the reference std for "TLC" in the Crude Drugs Test of the JP, 14th Edition.
Glycyrrhizic Acid Standard, 99.0+% (HPLC)071-02271 (20 mg) for Crude Drugs Determination  
Glycyrrhizic Acid Dipotassium Salt,
96.0〜102.0% (Absorptiometry)
072-03862 (25 g) for Biochemistry  
Glycyrrhizic Acid Monoammonium Salt
n-Hydrate,
90.0+% (as anhydrous)(Titration)
075-02171 (10 g) for Food Additives Test  
D-Glucuronyl-beta-1,2-D-glucuronic Acid
Dipotassium Salt
, 98.0+% (HPLC)
070-03101 (100 mg) for Biochemistry Hydrolysis of glycyrrhizin produces glycyrrhetinic acid and this product.
Glycyrrhetinic Acid, 98.0+% (Titration)072-02181 (10 g) for Food Additives Test An aglycon of glycyrrhizin, which is also called enoxolone.
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Wako Product Update No.20 [ page. 15 ]