6. Stimulant Detection Reagent
On-Column Chiral Derivatization Reagent for GC Separation of
Optical Isomeric Amphetamine & Methamphetamine
MTPA-Pyrazole is a reagent for on-column chiral derivatization designed to separate and analyze the
enantiomers of stimulants with a primary or secondary amine using a normal gas chromatography column.
In the past, separation and analysis of enantiomers required either the use of a chiral stationary phase column for analysis without pretreatment, or a pretreatment consisting of a reaction with a chiral derivatization reagent to form a diastereomer for analyses using a normal column.
MTPA-Pyrazole allows for derivatization reactions with stimulants with a primary or secondary amine by on-column injection, enantiomers can be quickly separated and analyzed without any pretreatment.
Preparation of MTPA-Pyrazole Solution: Dissolve MTPA-Pyrazole Reagent in ethyl acetate (Special Grade) to obtain a concentration of about 10 % (w/v).
Oven Temperature: 70°C (1 min.) to 280°C at 20°C per min.,
Injection Port Temperature: 200 ~ 250 °C
Injection Technique: split/splitless
Mass spectra of MTPA derivative of d,l-amphetamine
Total Ion Chromatogram of d,l-amphetamine and d,l-methamphetamine,
on- column chiral derivatized with MTPA-Pyrazole
Mass spectra of MTPA derivative of d,l-methamphetamine
|Description||Wako Cat. No. (Pkg. Size)||Storage||Shelf Life|
|MTPA-Pyrazole Reagent, 98.0+%(HPLC)
for Medicolegal Investigation
|134-15221 (100 mg)
130-15223 (1 g)
|Keep at -20°C in a dark place.
Filled with an inert gas.
|5 years after production|