2. Antibodies

Study for Human Aging Brain
Anti Phosphorylated α-Synuclein, Monoclonal Antibody (Clone No. pSyn #64)
Wako Cat. No. 015-25191 (50 µL) <for Immunochemistry>
Note: The 014-20281 was discontinued.
Keep at -20 °C
α-Synuclein in Lewy bodies (LBs) which are pathognomonic for Parkinson's disease (PD) and dementia with Lewy bodies (DLB), is phosphorylated at Ser129. We supply an antibody, which specifically reacts with human α-Synuclein having phosphorylated Ser129 residue and does not react with non-phosphorylated human α-Synuclein. This antibody is applicable to immunohistochemical and biological studies related to Parkinson's disease and LB pathology.

Subclass : Mouse IgG1
Specificity : Specific for human α-Synuclein with a phosphorylated Ser129. Does not cross-react with non-phosphorylated human α-Synuclein.
Working Dilution : 1 : 1000 ~ 1 : 10000 (Western blot and Immunohistochemistry)
[Immunohistochemistry of synucleinopathy lesions and Western blot analysis]


[References]
1) Fujiwara, H., et al.: Nature Cell Biology, 4, 160 (2002)
2) Saito, Y., et al.: J. Neuropathol Exp Neurol, 62, 644 (2003)
Research for Neurontransmitter and Alzheimer's disease
Anti Substance-P, Rabbit, affinity purified IgG fraction
Wako Cat. No. 016-13911 (1 mL) <for Immunochemistry>
Keep at -20°C

Substance P is a neuropeptide which is widely distributed in the periphery and the central nervous system, where it is co-localised with other neurotransmitters such as serotonin or dopamine and where it acts as a neuromodulator. Substance P has been proposed to play a role in the antiopathology of asthma, infl ammatory bowel disease, emesis, psoriasis, as well as neuropsychiatric disorders including pain syndromes (e.g. migraine and fibromyalgia) and affective disorders, anxiety disorders, schizophrenia and Alzheimer's disease.
Anti Substance P is isolated from rabbit antiserum against Substance P, and consists of the IgG fraction in 20 mM PBS solution (pH 7.2). The product is treated with Protein A column and affinity purified using Sepharose column.

Appearance: frozen
Protein content: 100 µL IgG/mL (A280nm)
Specificity: Does not cross-react with enkephalin, endorphin, or bradykinin
Antibody titer: 1 : 10,000 (EIA method)
[References]
1) Viamontes, G.I., et al.: "Antibodies to thymopoietin following implantation of paper disks derivatized with synthetic Cys-thymopoietin.", J. Immunol. Meth., 94, 13-17 (1986)
2) Hokfelt, T., et al.: "Dense plexus of substance P immunoreactive nerve terminals in eminentia medialis of the primate hypothalamus.", Proc. Nat. Acad. Sci, 75, 1013~ 15 (1978).
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Wako Product Update Bio-No.1 [ page. 8 ]