2. Antibodies

Anti soluble Guanylate Cyclase (sGC), MAb, NO insensitive (Clone: mAB28131)
Wako Catalog No. 017-18201 (20 µg (40 µL)) <for Immunochemistry>
Keep at -20°C
Prepared from culture supernatant and prepared in glycine-Tris solution (pH 7.4). Contains no preservatives and stabilizers.

Isotype: IgG1
Specifi cally reacts with rat, bovine and human β-subunit of sGC, but not strengthened in the reactivity on activation of sGC by NO.
Working Dilution:
Westernblot 1 : 5,000; Immunofl uorescence 1 : 250
Antibodies against Vesicular Glutamate Transporters
Anti Rat VGLUT-1, Rabbit
Wako Catalog No. 010-19771 (50 µg (100 µL)) <for Immunochemistry>
Keep at -20°C
Anti Rat VGLUT-2, Rabbit
Wako Catalog No. 017-19781 (50 µg (100 µL)) <for Immunochemistry>
Keep at -20°C
L-Glutamate is an excitatory chemical transmitter that plays an essential role in neuronal plasticity, behavior, learning and memory in the central nervous system. On the other hand, VGLUTs play an essential role in glutamate signal output through vesicular storage of L-glutamate. Three kinds of VGLUTs have been identifi ed so far. Recent studies have demonstrated that VGLUTs are also expressed in peripheral cells such as stomach, intestines, pancreas and testes. In particular, the discovery that glutamate is co-localized with glucagon in secretory granules in_cells of islets of Langerhans has been noted for new mechanism of blood glucose control. Anti Rat VGLUT, Rabbit can detect glutamatergic central nerves, peripheral nerves and nonneural cells.
Both antibodies are applicable to immunocytochemistry, immunoelectron microscopy and Western blotting.

Anti Rat VGLUT1, Rabbit

Raised against the GST fusion peptide encoding G 509-S 560 of the cytosolic regions of VGLUT1
Specifi city: Specifi c to rat, mouse, human and bovine VGLUT-1.No reactive to VGLUT-2
Working Conc.: Immunofl uorescence 1 : 1,500

Anti Rat VGLUT2, Rabbit

Raised against the GST fusion peptide encoding G 500-Y 582 of cytosolic regions of VGLUT2
Specificity: Specifi c to rat, mouse, human and bovine VGLUT-2. No reactive to VGLUT-1
Working Conc.: Immunofl uorescence 1 : 1,500
Application [1]: Immunofl uorescence

image
Application [2]: Immunoelectron microscopy

image Double immunoelectron microscopy in_cells of islets of Langerhans. Glucagon (5nm) and VGLUT2 (15nm) (arrowheads) are co-localized with secretory granules.
Photo by Dr. Mitsuko Hayashi (Yale Univ.)
[References]
1) Moriyama, Y. and Hayashi, M. : TRENDS Pharmacol. Sci. electric version, 24, 511 (2003).
2) Morimoto, R., Hayashi, M., Yatsushiro, S., Otsuka, M., Yamamoto, A. and Moriyama, Y. : J. Neurochem., 84, 382 (2003).
3) Hayashi, M., Morimoto, R., Yamamoto, A. and Moriyama, Y. : J. Histochem. Cytochem., 51, 1375 (2003).
4) Hayashi, M., Yamada, H., Uehara, S., Morimoto, R., Takeda, J., Yamamoto, A, and Moriyama, Y. : J. Biol. Chem., 277, 1966 (2003).
5) Hayashi, M., Otsuka, M., Morimoto, R., Muroyama, A., Uehara, S., Yamamoto, A., and Moriyama Y. : Diabetes, 52, 2066 (2003).
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Wako Product Update Bio-No.1 [ page. 10 ]