3. Apoptosis Research
Apoptosis Detection Kit by TUNEL method
Apoptosis in situ Detection Kit wako
Wako Cat. No. 293-71501 (40 tests) <for Apotosis Research >
Keep at -20°C
The kit is based on TUNEL [Terminal deoxynucleotidyl Transferase(TdT)-mediated dUTP nick end labeling] procedure, that is the addition of fl uorescein -dUTP to 3' -terminals of apoptotically fragmented DNA with TdT followed by immunochemical detection using anti-fl uorescein antibody conjugated with horseradish peroxidase (POD) and DAB as a substrate
Keep at -20°C
The kit is based on TUNEL [Terminal deoxynucleotidyl Transferase(TdT)-mediated dUTP nick end labeling] procedure, that is the addition of fl uorescein -dUTP to 3' -terminals of apoptotically fragmented DNA with TdT followed by immunochemical detection using anti-fl uorescein antibody conjugated with horseradish peroxidase (POD) and DAB as a substrate
[Features]
1. Rapid detection can be performed.
The whole process from the de-paraffi nizing step to the microscopic examination can be completed in about 2 hours.
The whole process from the de-paraffi nizing step to the microscopic examination can be completed in about 2 hours.
2. Complicated preparations of various reagents are not needed.
The kit contains the essential reagents required for detection of apoptosis.
The kit contains the essential reagents required for detection of apoptosis.
3. The kit shows a clear positive image with low background.
[Applicable samples]
- paraffin-embedded tissue sections
- frozen tissue sections
- neutralized formalin-fixed culture cells
[Kit Contents] (approx. 1 cm2 x 40 reactions)
| Protein Digestion Enzyme | 1 vial x 1 mL |
| TdT | 1 vial x 40 µL |
| TdT Substrate Solution | 1 vial x 4.4 mL |
| 100x POD-Conjugated Antibody | 1 vial x 44 µL |
| DNase I | 1 vial x 4 µL |
| 10x DNase I Reaction Buff er | 1 vial x 40 µL |
[TUNEL Staining]
induction (CPZ treatment) (x400)
Nuclei: Methyl Green Staining
(x200)
(x200)
(x200)
Nuclei: Methyl Green Staining
| Description | Catalog No. | Package Size |
|---|---|---|
| Apoptosis in situ Detection Kit wako | 298-60201 | 40 tests |
| Description | Catalog No. (Pkg. Size) | Storage |
|---|---|---|
| Deoxyribonuclease I (DNase I) (a kit content of Apopsis in situ Detection Kit) | 293-56601 (4 x 1 cm2) | Keep at -20°C |
| Lemosol® <limonene-based solvent as a xylene substitute> | 122-03991 (1 L) | Keep at RT |
| Lemosol® A <terpene-based solvent as a xylene substitute> | 120-04411 (1 L) | Protect from Light |
| Softmount <a mounting reagent containing LemosolR A> | 199-11311 (250 mL) | Protect from Light |
| 1x PBS (-) Powder (0.01 mol/L, pH 7.2 ~ 7.4) | 162-19321 (for 1 L x 20) | Keep at RT |
| Methyl Green Solution (0.5 w/v%) | 138-12701 (100 mL) | Keep at 2~10°C |
Wako Product Update Bio-No.1 [ page. 11 ]