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3. Apoptosis Research

Apoptosis Detection Kit by TUNEL method
Apoptosis in situ Detection Kit wako
Wako Cat. No. 293-71501 (40 tests) <for Apotosis Research >
Keep at -20°C
The kit is based on TUNEL [Terminal deoxynucleotidyl Transferase(TdT)-mediated dUTP nick end labeling] procedure, that is the addition of fl uorescein -dUTP to 3' -terminals of apoptotically fragmented DNA with TdT followed by immunochemical detection using anti-fl uorescein antibody conjugated with horseradish peroxidase (POD) and DAB as a substrate

[Features]
1. Rapid detection can be performed.
The whole process from the de-paraffi nizing step to the microscopic examination can be completed in about 2 hours.
2. Complicated preparations of various reagents are not needed.
The kit contains the essential reagents required for detection of apoptosis.
3. The kit shows a clear positive image with low background.
[Applicable samples]
  • paraffin-embedded tissue sections
  • frozen tissue sections
  • neutralized formalin-fixed culture cells
[Kit Contents] (approx. 1 cm2 x 40 reactions)
Protein Digestion Enzyme1 vial x 1 mL
TdT1 vial x 40 µL
TdT Substrate Solution1 vial x 4.4 mL
100x POD-Conjugated Antibody1 vial x 44 µL
DNase I1 vial x 4 µL
10x DNase I Reaction Buff er1 vial x 40 µL

[TUNEL Staining]

image

Cultured cell CHO-K1: after Apoptosis
induction (CPZ treatment) (x400)
Nuclei: Methyl Green Staining

image

Rat small intestine (x400) Nuclei: Methyl Green Staining

image

Rat testicle: DAB Intensifying Staining
(x200)

image

Human T cell lymphoma: HE Staining
(x200)

image

Human B cell lymphoma: HE Staining
(x200)

image

Human gastric cancer (x200)
Nuclei: Methyl Green Staining
Description Catalog No. Package Size
Apoptosis in situ Detection Kit wako 298-60201 40 tests
Related Products
DescriptionCatalog No. (Pkg. Size)Storage
Deoxyribonuclease I (DNase I) (a kit content of Apopsis in situ Detection Kit)293-56601 (4 x 1 cm2)Keep at -20°C
Lemosol® <limonene-based solvent as a xylene substitute>122-03991 (1 L)Keep at RT
Lemosol® A <terpene-based solvent as a xylene substitute>120-04411 (1 L)Protect from Light
Softmount <a mounting reagent containing LemosolR A>199-11311 (250 mL)Protect from Light
1x PBS (-) Powder (0.01 mol/L, pH 7.2 ~ 7.4) 162-19321 (for 1 L x 20)Keep at RT
Methyl Green Solution (0.5 w/v%)138-12701 (100 mL)Keep at 2~10°C
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Wako Product Update Bio-No.1 [ page. 11 ]