4. Cell Separation

for Lymphocyte Separation
Iron Powder, from Iron Carbonyl, 99.0+% (Titration)
Wako Cat. No. 098-02222 (25 g) <for Lymphocyte Separation>
Keep at RT.
The product is a very fine uniform iron powder used for macrophage elimination.
CAS No. 7439-89-6
Appearance: Grayish black, powder
Particle Size: approx. 6 μm
Bulk specific gravity: 3 ~ 4 g/mL
Solubility: Soluble in dil.HCl, dil.H2SO4, dil.HNO3 with producing hydrogen gas.
[References]
(1) Lee, K. C., et al.: "Requirement for accessory cells in the antibody response to T cell-independent antigens in vitro", Eur. J. Immunol., 6, 63 (1976)
(2) Thierfelder, S.: "A METHOD FOR THE ISOLATION OF HUMAN LYMPHOCYTES", Vox Sang, 9, 447-54 (1964)
for T-cell Separ
Nylon Fiber
Wako Cat. No. 146-04231 (5 x 2 g); 142-04233 (100 g) <for T-cell Separation>
Keep at RT

Appearance: White ~ slightly pale yellow, fibrous
Divalent cations (as Ca2+): 10+ ug/g
Solubility: Soluble in dil.HCl, dil.H2SO4, dil.HNO3 with producing hydrogen gas.

A simple method for the preparation of highly enriched, unselected and unaltered populations of T cells has been described by Julius. By packing nylon wool fiber into columns, efficient isolation of T cells from antisera without B cell contamination is possible. Wako has improved upon the same method, offering nylon wool fiber which is free of all toxic substances, in addition, pre-washed, pre-packaged nylon wool fiber comes ready to use in sterilized 10cc-columns, saving hours of preparation. Prepackaged columns also eliminate variability in column packing, insuring consistent cell elution rates.
Nylon Fiber Columns
Nylon Fiber Column T
Wako Cat. No. 147-06721 (10 syringes x 0.5 g) <for mouse T-cell Separation>
Keep at RT
Nylon Fiber Column T (L-Type)
Wako Cat. No. 143-07041 (10 syringes x 1 g) <for human, rabbit and rat T-cell Separation>
Keep at RT
We offer two sizes of Nylon Fiber Column T; Nylon Fiber Column T is for separation of T-cells of mouse lymphocytes and the L-type is for that of a large amount of suspended solution, especially rat splenic lymphocytes, and peripheral blood lymphocytes of rabbit and human.

[Features]
1. Simple procedure.
2. High reproducibility.
3. Sterilized.
4. Using a high quality nylon fiber.
Cell Recovery:
13 ~ 25% (mouse) when Nylon Fiber Column T was used.
25 ~ 35% (rat), 20 - 30% (human) and 25 ~ 30% (rabbit) when Nylon Fiber Column T (L-Type) was used.
B-cell contamination: Less than 15%
[References]
Julius, M.G., E. Simpson, and R.A. Herzenberg: "A rapid method for the isolation of functional thymus-derived murine lymphocytes", Eur. J. Immun., 3, 645-649 (1973).
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Wako Product Update Bio-No.1 [ page. 13 ]