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5. Chemiluminescent Probes

Green Chemiluminescent probe for superoxide anions
Green Chemiluminescent CD
Wako Catalog No. 075-05111 (1 mg)
Keep at -20°C
Green Chemiluminescent CD is a highly sensitive chemiluminescence probe, which was developed by Dr. Teranishi of Mie University, Japan. This probe reacts with superoxide anion and produces luminescence at long wavelengths. Therefore the luminescence remains nearly unaffected by biomaterials. Additionally, this probe is more sensitive than other probes which produce luminescence at long wavelengths.
1. High luminescence intensity
2. Luminescence at long wavelengths (530 nm)
Soluble to hot methanol : H2O (1:1) containing 0.1% TFA.
Teranishi, K. and Nishiguchi, T. : Anal. Biochem., 325, 185 (2004).
L-012 Sodium Salt, 98.0+% (HPLC)
[8-Amino-5-chloro-7-phenylpyrido [3,4-d] pyridazine-1,4-(2H,3H) dione sodium salt]
Wako Catalog No. 120-04891
Keep at -20°C,Solid

L-012, which is a highly sensitive chemiluminescent (CHL) probe, is more active than luminol. L-012 reacts with various types of reactive oxygen species generated by activated neutrophils in human blood and oral cavity, and from peritoneal cavity of the rat. This product can be applied to any other EIA that uses horseradish peroxidase to improve sensitivity.
1) "Improved Enzyme Immunoassay for Human Basic Fibroblast Growth Factor Using A New Enhanced Chemiluminescence System", Ii, M., et al., Biochem. Biophys. Res. Comm., 193 (2), 540-545 (1993)
2) "A New Sensitive Chemiluminescence Probe, L-012, for Measuring The Production of Superoxide Anion by Cells", Nishinaka, Y., et al., Biochem. Biophys. Res. Comm., 193 (2), 554-559 (1993)
3) "Analysis of Reactive Oxygen Species Generated by Neutrophils Using a Chemiluminescence Probe L-012", Imada, I., et al., Analytical Biochemistry, 271, 53-58 (1999)
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Wako Product Update Bio-No.1 [ page. 14 ]