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6. Fluorescent Probes

Description Cell Permeability Description (Grade) Physical data, etc. Wako Cat. No. (Pkg. Size) Note
Thiol/Selenol selective probe x
(for Cellbiology)
BES-Thio
C28H18N2O11S = 590.51
<Fluorescence> λex: 495 nm; λem: 530 nm
025-15481 (1 mg) Keep at RT
See the page #15.
H2O2 Probes O
<Detection of cellderived H2O2>
(for Cellbiology)
BES-H2O2-Ac *1 , 94.0+% (HPLC)
[3'-O-Acetyl-6'-O-pentafluorobenzenesulfonyl-2'-7'- difl uorofl uorescein] C28H11F7O8S = 640.44
Solubility: Soluble in DMSO, DMF and acetonitrile
<Fluorescence> λex: 485 nm; λem: 530 nm
029-15381 (1 mg) Keep at RT
See the page #16.
x
(for Cellbiology)
BES-H2O2 (cell-impermeant)
C26H9O7F7S = 598.40
Solubility: Soluble in DMSO
<Fluorescence> λex: 485 nm; λem: 530 nm
021-16201 (1 mg)
Superoxide selective probe O
<Detection of cellderived superoxide>
(for Cellbiology)
BES-So-AM *2 ,98.0+ % (HPLC)
C31H19O13NF4S = 721.54
<Fluorescence> λex: 505 nm; λem: 544 nm
021-15601 (1 mg) Keep Keep at RT
See the page #17.
x
(for Cellbiology)
BES-So (cell-impermeant)
C28H15O11NF4S = 649.48
Solubility: Soluble in DMSO
<Fluorescence> λex: 505 nm; λem: 544 nm
028-16211 (1 mg)
*1: Previous name for BES-H2O2-AC: BES-H2O2 ; *2: Previous name for BES-So-AM: BES-So
Fluorescent Bioprobe for Visualization of PSA in Living Cells
(for Cellbiology)
DAMPAQ-22
C31H32O4N4S = 556.68
CAS No. 519183-48-3
<Fluorescence> λex: 320 nm; λem: 510 nm
049-30761 (2 mg) Keep at RT
See the page #32.
Mg2+-Selective Fluoroionophore
(for Cellbiology)
KMG-20-AM
C19H19NO6 = 357.36
<Fluorescence> λex: 320 nm; λem: 510 nm
110-00711 (1 mg)
116-00713 (5 mg)
Keep at -20℃
See the page #18.
Thiol / Selenol selective fl uorescent probe
BES-Thio
Wako Catalog No. 075-05111 (1 mg)
Keep at -20°C
Several chemiluminescent and fl uorescent reagents are known as a thiol group detection reagent, and used for detection of thiol groups or measurement of cholinesterase activity.
Most of these reagents have a low hydrophilicity, so separate reaction steps, enzyme and detection reactions, are required.
BES-Thio has a high hydrophilicity and can be used in aqueous solution. This feature makes it easy to measure the enzyme activity such as cholinesterase using acetylthiocholine or butyrylthiocholine as a substrate.
Furthermore, by pH adjustment, BES-Thio can also detect selenol groups, which sulfur (S) in thiol group is substituted by selenium (Se), and can be used for selenoprotein detection reagent.
image
[Features]
1. High hydrophilicity
2. Respond to thiol at pH 7.4
3. Respond to selenol at pH 5.8
[References]
1) Maeda, H., Matsuno, H., Ushida, M., Katayama, K., Saeki, K. and Itoh, N.: Angew. Chem. Int. Ed., 44, 2922 (2005).
2) Maeda, H., Katayama, K., Matsuno, H. and Uno, T.: Angew .Chem. Int. Ed., 45, 1810 (2006).
3) Maeda, H: Wako Jun-yaku Jiho, 73 (3), 2 (2005) (written in Japanese).
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Wako Product Update Bio-No.1 [ page. 15 ]