6. Fluorescent Probes

Description Cell Permeability Description (Grade) Physical data, etc. Wako Cat. No. (Pkg. Size) Note
Thiol/Selenol selective probe x
(for Cellbiology)
C28H18N2O11S = 590.51
<Fluorescence> λex: 495 nm; λem: 530 nm
025-15481 (1 mg) Keep at RT
See the page #15.
H2O2 Probes O
<Detection of cellderived H2O2>
(for Cellbiology)
BES-H2O2-Ac *1 , 94.0+% (HPLC)
[3'-O-Acetyl-6'-O-pentafluorobenzenesulfonyl-2'-7'- difl uorofl uorescein] C28H11F7O8S = 640.44
Solubility: Soluble in DMSO, DMF and acetonitrile
<Fluorescence> λex: 485 nm; λem: 530 nm
029-15381 (1 mg) Keep at RT
See the page #16.
(for Cellbiology)
BES-H2O2 (cell-impermeant)
C26H9O7F7S = 598.40
Solubility: Soluble in DMSO
<Fluorescence> λex: 485 nm; λem: 530 nm
021-16201 (1 mg)
Superoxide selective probe O
<Detection of cellderived superoxide>
(for Cellbiology)
BES-So-AM *2 ,98.0+ % (HPLC)
C31H19O13NF4S = 721.54
<Fluorescence> λex: 505 nm; λem: 544 nm
021-15601 (1 mg) Keep Keep at RT
See the page #17.
(for Cellbiology)
BES-So (cell-impermeant)
C28H15O11NF4S = 649.48
Solubility: Soluble in DMSO
<Fluorescence> λex: 505 nm; λem: 544 nm
028-16211 (1 mg)
*1: Previous name for BES-H2O2-AC: BES-H2O2 ; *2: Previous name for BES-So-AM: BES-So
Fluorescent Bioprobe for Visualization of PSA in Living Cells
(for Cellbiology)
C31H32O4N4S = 556.68
CAS No. 519183-48-3
<Fluorescence> λex: 320 nm; λem: 510 nm
049-30761 (2 mg) Keep at RT
See the page #32.
Mg2+-Selective Fluoroionophore
(for Cellbiology)
C19H19NO6 = 357.36
<Fluorescence> λex: 320 nm; λem: 510 nm
110-00711 (1 mg)
116-00713 (5 mg)
Keep at -20℃
See the page #18.
Thiol / Selenol selective fl uorescent probe
Wako Catalog No. 075-05111 (1 mg)
Keep at -20°C
Several chemiluminescent and fl uorescent reagents are known as a thiol group detection reagent, and used for detection of thiol groups or measurement of cholinesterase activity.
Most of these reagents have a low hydrophilicity, so separate reaction steps, enzyme and detection reactions, are required.
BES-Thio has a high hydrophilicity and can be used in aqueous solution. This feature makes it easy to measure the enzyme activity such as cholinesterase using acetylthiocholine or butyrylthiocholine as a substrate.
Furthermore, by pH adjustment, BES-Thio can also detect selenol groups, which sulfur (S) in thiol group is substituted by selenium (Se), and can be used for selenoprotein detection reagent.
1. High hydrophilicity
2. Respond to thiol at pH 7.4
3. Respond to selenol at pH 5.8
1) Maeda, H., Matsuno, H., Ushida, M., Katayama, K., Saeki, K. and Itoh, N.: Angew. Chem. Int. Ed., 44, 2922 (2005).
2) Maeda, H., Katayama, K., Matsuno, H. and Uno, T.: Angew .Chem. Int. Ed., 45, 1810 (2006).
3) Maeda, H: Wako Jun-yaku Jiho, 73 (3), 2 (2005) (written in Japanese).
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Wako Product Update Bio-No.1 [ page. 15 ]