6. Fluorescent Probes
Highly Selective Fluorescent Probe for Hydrogen Peroxide
BES-H2O2-Ac (cell-permeant), BES-H2O2 (cell-impermeant)
Reactive oxygen species (ROS) such as superoxide (O2 -.), hydrogen peroxide (H2O2), and the hydroxyl radical (HO . ) are important mediators of pathological processes in various diseases. 2',7'-Dichlorofl uorescein (DCFH) and its diacetyl derivative have been widely used as fl uorescent probes for measuring cell-derived H2O2, but these compounds suffer from the major drawback that they are poorly selective toward H2O2.
Wako has launched two kinds of BES-H2O2, which are probes for cell-derived H2O2 and cell-impermeat H2O2 with high selectively.
BES-H2O2-Ac is applicable to clarifying cell response as well as dynamic function of H2O2 with diseases.
Fluorescent images of Jurkat T cells with BES- H2O2 -Ac and the same-fi eld phase-contrast images
Jurkat T cells were cultured in a medium with 50µM BES-H2O2-Ac for 1 hour. Then, one group of them was cultured in a medium
with 5mM butyric acid (H2O2-produced stimulation), whereas the other in a medium without butyric acid (No H2O2-produced
stimulation), each for 1 hour. (Courtesy: Professor Hatsuo Maeda, PhD., School of Pharmacy, Hyogo Univ. of Health Sciences)
1) Maeda, H., Fukuyasu, Y., Yoshida, S., Fukuda, M. Saeki, K.. Matsuno, H., Yamauchi, Y., Yoshida, K., Hirata, K. and Miyamoto, K. : Angew. Chem. Int. Ed., 43, 2389 (2004).
2) Maeda, H., Matsuura, S., Nishida, M., Senba, T.,Yamauchi, Y. and Ohmori, H. : Chem. Pharm. Bull., 49, 294 (2001).
Wako Product Update Bio-No.1 [ page. 16 ]