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6. Fluorescent Probes

Fluorescent Bioprobe for Visualization of Puromycin-Sensitive Aminopeptidase
(PSA) in Living Cells

Wako Catalog No. 049-30761 (2 mg) <for Cellbiology>
Keep at RT

See the page #32.
Mg2+-selective Fluoroionophore

Figure : Dynamics of Mg2+ probe (KMG-20-AM) in neuron by addition of K+
Wako Catalog No. 110-00711 1 mg
Wako Catalog No. 116-00713 5 mg
Keep at -20℃

Dynamic distribution of Mg2+ in living cells can be done due to selective recognition of Mg2+ by KMG-20-AM. KMG-20-AM is much less reactive to Ca2+ than Mg2+.
KMG-20-AM enables accurate measurement of Mg2+ because it has very much low affi nity to Ca2+ compared to Mg2+.
Appearance: Brown, powder
Assay (HPLC): 95+ %

1. Mg2+-imaging without interference of Ca
2. Precise observation of Mg2+ distribution by Fluorescent Microscopy
3. Direct observation of Mg2+ ion dynamics in living cells
[ Figure : ]
Absorption spectra (A) and fl uorescence spectra (B) of 10.0 µM KMG-20-AM before and after the addition of MgCl2 at 37℃ in 10.0 mM HEPES, 120.0 mM KCl, 20.0 mM NaCl (pH 7.2). [MgCl2]=0, 0.1, 0.5, 1, 2, 5, 10, 20, 50, 100, 200, 500 mM. Excitation at 445 nm for the fl uorescence measurements.

C19H19NO6 = 357.36
λex : 440 nm; Mem : 500 ~ 530 nm


[ Figure : ]
Responses of fl uorescence intensity of KMG-20-AM and Magnesium Green for Ca2+. Arrow indicates the timing of 10 µM CaCl2 addition ([Ca2+] increased from 140 to 850 nm).

1) Nagashima, H., Tohda, K., Matsunari, Y.,Tsunakwa, Y., Watanabe, K., Inoue, H. andSuzuki, K.: Anal. Lett., 23, 1993(1990).
2) Suzuki, K., Watanabe, K., Matsumoto, Y.,Kobayashi, M., Sato, S., Siswanta, D. andHisamoto, H.: Anal. Chem., 67, 324(1995).
3) Suzuki, Y., Saito, N., Komatsu, H., Citterio,D., Kitamura, Y., Kubota, T., Oka, K. andSuzuki, K.: Anal. Sci., 17, i1451(2001).
4) Suzuki, Y., Komatsu, H., Ikeda, T., Saito, N., Araki, S., Citterio, D., Hisamoto, H., Kitamura,Y., Kubota, T., Nakagawa, J., Oka, K. and Suzuki, K.: Anal. Chem., 74, 1423(2002).
5) Haugland, R. P.: "Handbook of FluorescentProbes and Research Products, 7thed. ",Molecular Probes Inc.
6) Kubota, T., Tokuno, K., Nakagawa, J., Kitamura, Y., Ogawa, H., Suzuki, Y., Suzuki,K. and Oka, K.: Biochem. Biophys. Res.Commun., 303, 332(2003).
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Wako Product Update Bio-No.1 [ page. 18 ]