This website uses cookies. By using the site you are agreeing to our Privacy Policy.

7. microRNA Research

for Immunoprecipitation, Western Blot, Immunocytochemistry
Anti Mouse Ago2, Monoclonal Antibody(Clone No. 2D4)
Wako Cat. #014-22023 (50µL); 018-22021 (100µL)
Keep at 2~10℃


Argonaute2 (Ago2) was isolated as one of the main components of RISC (RNA-induced silencing complex). Ago2 captures siRNA and microRNA which are working as a guide molecule for interaction with target mRNAs in RNAi pathway. In this pathway, Ago2 catalyzes the nicking of target mRNAs and binding between RISC and target mRNAs. This monoclonal antibody is not only used for western blot and immunocytochemistry (ICC), but also immunoprecipitation (IP) of mAgo2.
ApplicationWorking Dilution
Western Blot1 : 200 - 1 : 1,000
Immunoprecipitation5~10µg / IP
Immunocytochemistry1 : 100 - 1 : 500

OrganismsMouseHamsterRatHuman
Cell P388D1
NIH-3T3
CHO SCC-131 NCI-H460
Western BlotO O O X
ImmunoprecipitationO O O X
ImmunocytochemistryO
(NIH-3T3)
NTNTX
microRNA Purifi cation O
(P388D1)
O O X
[Features]
1. For IP, ICC, Western Blot
2. Cross reactivity with Ago2 protein of rat & hamster
3. For purifi cation of RNA captured by RISC
Concentration (protein): Indicated on the label.
Formulation: 0.05% Sodium Azide, 10% Glycerol
with 1 x TBS, pH7.4.
Subclass: IgG1
Antigen: Synthesized peptide of N terminal mouse Ago2.
Storage: 2~10℃ in the dark. Avoid the freeze and thaw.
[References]
Mishima, T., et al.: Reproduction, Sep. 4, [E pub ahead of print]
APPLICATION DATA
Immunoprecipitation of mAgo2 protein from P388D1 cell line
image
[ Figure 1 ]
I mmunoprecipitation of mAgo2 protein from P388D1 cell line by using 20µL 10% Protein G slurry immobilized with 5µg this antibody (2D4). The band of endogenous mAgo2 protein was detected in approximately 100kDa by using silver staining and western blot. The 1/1,000 diluted this antibody was used as the 1st antibody for western blot. Cell number was 5 x106 cells.
Immunocytochemistry of hAgo2 protein of HeLa cell line
image
[ Figure 2 ]
Immunocytochemistry of mAgo2 protein of NIH-3T3 cell line by using 1/300 diluted this antibody. mAgo2 protein was localized in cytoplasm.

Specifi city of anti hAgo2, Monoclonal Antibody (4G8)
image
[ Figure 3 ]
Immunoprecipitation of mAgo2 protein from P388D1 cell line by using 20µL 10% Protein G slurry immobilized with 5µg this antibody. The purifi ed microRNA fraction from P388D1 cell lines were specifi cally detected by Urea-PAGE. Cell number was 5 x106 cells.


image
[ Figure 4 ]
Immunoprecipitation of Ago2 protein from NIH-3T3(Mouse), SCC- 131(Rat) and CHO(Hamster) cell line by using 20µL 10% Protein G slurry immobilized with 5µg this antibody (2D4). The band of endogenous mAgo2 protein was detected in approximately 100kDa by using silver staining and western blot. The 1/1,000 diluted this antibody was used as the 1st antibody for western blot. Cell number was 5 x106 cells.
page top
backindexnext
Wako Product Update Bio-No.1 [ page. 23 ]