8. Physiological Active Substances

Anisomycin, 96.0+ % (HPLC)
Wako Cat. No. 017-16861 (10 mg); 013-16863 (50 mg); 011-16864 (250 mg) <for Biochemistry>
Keep at -20℃


Antibiotic. Activator of p38 and MAP kinases. Synergistic with growth factors and phorbol esters to superinduce cFos and cJun, by acting as a potent signalling agonist. Induces apoptosis in the human monoblastoid cell line. Used in the eradication of bean mildew. Inhibits other pathogenic fungi in plants.
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Source:Isolated from Streptomyces griseolus
Appearance:White ~ slightly yellow, crystalline powder ~ powder
Solubility:Soluble in DMSO (25mg/mL), 100% ethanol, methanol or ethyl acetate (10mg/mL)
Aristeromycin
Wako Cat. No. 015-09691 (5 mg) <for Biochemistry>
Keep at -20℃


The product is an antibiotic which inhibits the growth of plant pathogens such as Xanthomonas oryzae and Piricuraria oryzae.
Carbocyclic nucleoside antibiotic. It inhibits the synthesis of AMP in mammalian cells and S-adenosylhomocysteine hydrolase activity.
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Source:Streptomyces citricolor
Appearance:White ~ grayish white, powder or mass
Solubility:Soluble in water and N, N-dimethylformamide
Bafi lomycin A1, 95.0+% (HPLC)
Wako Cat. No. 023-11641 (100µg); 029-11643 (1 mg) <for Biochemistry>
Keep at -20℃


Bafi lomycin A1, isolated from Streptomyces sp., is a macrolide antibiotic which inhibits vacuolar-type H+-ATPases with a high degree of specifi city.
Bafi lomycin A1 thus serves as an ideal tool for distinguishing among the different types of ATPases that exist in eukaryotic cells. Among them,vacuolar-type ATPase (V-ATPases) are widely distributed in the central vacuolar system, consisting of endosomes, trans-Golgi network, lysosomes, and secretion granules. While V-ATPases are extremely sensitive to the antibiotic and are affected by nanomolar concentrations, F-ATPases (F1F0-type) are unaffected, and P-ATPases (E1E2-type) are only moderately affected at the same concentration.
Source:Streptomyces citricolor
Appearance: White, Crystals ~ powder or fi lm
Solubility: Soluble in DMSO, ethanol and ethyl acetate. Slightly soluble in water.
[References]
1) Bowman, E. J., Siebers, A. and Altendorf, K.: Proc. Natl. Acad. Sci., U.S.A., 85, 7972 (1988)
2) Moriyama, Y. and Nelson, N.: J. Biol. Chem., 264, 18445 (1989)
3) Nelson, N. and Taiz, L.: Trends Biochem. Sci., 14, 113 (1989)
4) Hanada, H., Moriyama, Y., Maeda, M. and Futai, M.: Biochem. Biophys. Res. Commun., 170, 873 (1990)
5) Moriyama, Y. and Futai, M.: J. Biol. Chem., 265, 9165 (1990)
6) Moriyama, Y., Maeda, M. and Futai, M.: J. Biochem., 108, 689 (1990)
7) Sundquist, K., Lakkakorpi, P., Wallmark, B. and Vaeaenaenen, K.: Biochem. Biophys. Res. Commun., 168, 309 (1990)
8) Umata, T., Moriyama, Y., Futai, M. and Mekada, E.: J. Biol. Chem., 265, 21940 (1990)
9) Oda, K., Nishimura, Y., Ikehara, Y., Kato, K.: Biochem. Biophys. Res. Commun., 178, 369 (1991)
10) Yoshimori, T., Yamamoto, A., Moriyama, Y., Futai, M. and Tashiro, Y.: J. Biol. Chem., 266, 17707 (1991)
11) Werner, G., Hagenmaier, H., Drautz, H., Baumgartner, A. and Zaehner, H.: J. Antibiot., (Tokyo), 37, 110 (1984)
Bleomycin Hydrochloride
Wako Cat. No. 028-07801 (10 mg) <for Biochemistry>
Keep at 2~10℃


This is an anticancer antibiotic acting on various cancers. It inhibits DNA synthesis in Escherichia coli, HeLa cells and Ehrlich cancer cells and, moreover, inhibits their cell divisions at a low concentration.
Appearance::White ~ pale yellow, powder or small mass
Potency:: 1,400 ~ 2,000 ug/mg (calculated on the dried basis)
Solubility: Soluble in water. Slightly soluble in ethanol. Practically insoluble in ether.
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Wako Product Update Bio-No.1 [ page. 25 ]