8. Physiological Active Substances
Bacterial Protein Synthesis Inhibitor
Gentamicin Sulfate
Wako Cat. No. 073-02971 (250 mg); 079-02973 (1 g); 077-02974 (5 g); 071-02972 (25 g) <for Biochemistry>
Wako Cat. No. 078-04981 (250 mg); 074-04983 (1 g); 072-04984 (5 g) <for Molecular Biology>
Keep at 2~10℃
This product is an antibiotic that has an antimicrobial activity against gram positive and nagative bacteria. It inhibits the initiation of protein synthesis of bacteria by acting as a ribosome to induce misreading of codons. It is a mixture of gentamicin C1, C2, and C1a.
As a reagent for molecular biology, it has been confi rmed for DNase and RNase activities.
Wako Cat. No. 078-04981 (250 mg); 074-04983 (1 g); 072-04984 (5 g) <for Molecular Biology>
Keep at 2~10℃
This product is an antibiotic that has an antimicrobial activity against gram positive and nagative bacteria. It inhibits the initiation of protein synthesis of bacteria by acting as a ribosome to induce misreading of codons. It is a mixture of gentamicin C1, C2, and C1a.
As a reagent for molecular biology, it has been confi rmed for DNase and RNase activities.
| Source: | Micromonospora purpurea |
| Appearance: | White ~ slightly pale brown, powder |
| Potency: | 590+ ug/mg (calculated on the dried basis) |
| Solubility: | Freely soluble in water. Practically insoluble in ethanol |
Highly Selective γ-Glutamyl transpeptidase (GGT) Inhibitor
GGsTop™
Wako Cat. No. 075-05471 (10 mg) <for Cellbiology>
Keep at -20℃
Keep at -20℃
GGsTop™ is a highly selective γ-Glutamyl Transpeptidase (GGT) inhibitor. While
acivicin [AT-125], which is widely used as a GGT inhibitor also inhibits asparagine
synthetase (GA family), GGsTop™ does not inhibit the asparagine synthetase.
[Features]]
Inhibitory activities of GGsTop™ and aciviin
toward E. coli and human GGT. Each numeric
value shows second-order rate constant for
enzyme inactivation1).
1. High specifi city to GGT
Acivicin deactivates more than 90% of 100UM of E. coli asparagine synthetase for 2 hours. On the other hand, GGsTop™ did not deactivates even the 10mM enzyme.
Acivicin deactivates more than 90% of 100UM of E. coli asparagine synthetase for 2 hours. On the other hand, GGsTop™ did not deactivates even the 10mM enzyme.
2. High inhibitory activity to human GGT
| to human GGT | to E. coli GGT | |
| GGsTop™ | 51 | 170 |
| Acivicin [AT-125] | 0.40 | 4,200 |
3. Low toxicity
On acute toxicity test, there is no toxicity with intravenously-infused GGsTop™ (30mg/kg). On the other hand, acivicin has severe toxicity to the CNS.
On acute toxicity test, there is no toxicity with intravenously-infused GGsTop™ (30mg/kg). On the other hand, acivicin has severe toxicity to the CNS.
4. Chemically stable
The reconstituted neutral or acid aqueous solution such as 0.1% TFA solution and 0.1N HCl solution is stable for 1 month at room temperature for NMR analysis.
The reconstituted neutral or acid aqueous solution such as 0.1% TFA solution and 0.1N HCl solution is stable for 1 month at room temperature for NMR analysis.
[References]
Han, L., Hiratake, J., Kamiyama, A. and Sakata, K.: "Design, synthesis, and evaluation of γ-phosphono diester analogues of glutamate as highly potent inhibitors and active site probes of γ-glutamyl transpeptidase", Biochemistry, 46, 1432-47 (2007).
Han, L., Hiratake, J., Kamiyama, A. and Sakata, K.: "Design, synthesis, and evaluation of γ-phosphono diester analogues of glutamate as highly potent inhibitors and active site probes of γ-glutamyl transpeptidase", Biochemistry, 46, 1432-47 (2007).
A synthetic analog of Coenzyme Q10
Idebenone
Wako Cat. No. 096-05001 (100 mg) <for Biochemistry>
Keep at 2~10℃
Idebenone is known to act on the central nervous system (CNS) and ameliorate cerebral apoplexy, cerebral ischemia with affective disorder, tetraplegia, and impaired passive avoidance response.
Keep at 2~10℃
Idebenone is known to act on the central nervous system (CNS) and ameliorate cerebral apoplexy, cerebral ischemia with affective disorder, tetraplegia, and impaired passive avoidance response.
| Appearance: | Yellowish red ~ orange, crystals ~ crystalline powder or mass |
| Solubility: | Soluble in ethanol. |
[References]
p.282, Pharmaceutical Handbook, the 5th edition, edited by Osaka Pharmaceutical Association.
p.282, Pharmaceutical Handbook, the 5th edition, edited by Osaka Pharmaceutical Association.
Wako Product Update Bio-No.1 [ page. 29 ]
Related Wako Products:
- TFAP:
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