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8. Physiological Active Substances

Bacterial Protein Synthesis Inhibitor
Gentamicin Sulfate
Wako Cat. No. 073-02971 (250 mg); 079-02973 (1 g); 077-02974 (5 g); 071-02972 (25 g) <for Biochemistry>
Wako Cat. No. 078-04981 (250 mg); 074-04983 (1 g); 072-04984 (5 g) <for Molecular Biology>
Keep at 2~10℃

This product is an antibiotic that has an antimicrobial activity against gram positive and nagative bacteria. It inhibits the initiation of protein synthesis of bacteria by acting as a ribosome to induce misreading of codons. It is a mixture of gentamicin C1, C2, and C1a.
As a reagent for molecular biology, it has been confi rmed for DNase and RNase activities.
Source:Micromonospora purpurea
Appearance:White ~ slightly pale brown, powder
Potency:590+ ug/mg (calculated on the dried basis)
Solubility:Freely soluble in water.
Practically insoluble in ethanol
Highly Selective γ-Glutamyl transpeptidase (GGT) Inhibitor
Wako Cat. No. 075-05471 (10 mg) <for Cellbiology>
Keep at -20℃

GGsTop™ is a highly selective γ-Glutamyl Transpeptidase (GGT) inhibitor. While acivicin [AT-125], which is widely used as a GGT inhibitor also inhibits asparagine synthetase (GA family), GGsTop™ does not inhibit the asparagine synthetase.
1. High specifi city to GGT
Acivicin deactivates more than 90% of 100UM of E. coli asparagine synthetase for 2 hours. On the other hand, GGsTop™ did not deactivates even the 10mM enzyme.
2. High inhibitory activity to human GGT
  to human GGT to E. coli GGT
GGsTop™ 51 170
Acivicin [AT-125] 0.40 4,200
Inhibitory activities of GGsTop™ and aciviin toward E. coli and human GGT. Each numeric value shows second-order rate constant for enzyme inactivation1).
3. Low toxicity
On acute toxicity test, there is no toxicity with intravenously-infused GGsTop™ (30mg/kg). On the other hand, acivicin has severe toxicity to the CNS.
4. Chemically stable
The reconstituted neutral or acid aqueous solution such as 0.1% TFA solution and 0.1N HCl solution is stable for 1 month at room temperature for NMR analysis.
Han, L., Hiratake, J., Kamiyama, A. and Sakata, K.: "Design, synthesis, and evaluation of γ-phosphono diester analogues of glutamate as highly potent inhibitors and active site probes of γ-glutamyl transpeptidase", Biochemistry, 46, 1432-47 (2007).
A synthetic analog of Coenzyme Q10
Wako Cat. No. 096-05001 (100 mg) <for Biochemistry>
Keep at 2~10℃

Idebenone is known to act on the central nervous system (CNS) and ameliorate cerebral apoplexy, cerebral ischemia with affective disorder, tetraplegia, and impaired passive avoidance response.
Appearance: Yellowish red ~ orange, crystals ~ crystalline powder or mass
Solubility: Soluble in ethanol.
p.282, Pharmaceutical Handbook, the 5th edition, edited by Osaka Pharmaceutical Association.
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Wako Product Update Bio-No.1 [ page. 29 ]