1. Kits

1-g) C-Peptide

C-Peptide

image

Insulin C-peptide (InsC-peptide) is derived from proinsulin by processing in vivo, and released into blood in almost equal molarity to insulin. By measurement of InsC-peptide in the serum, insulin secretion on pancreatic b cells in insulinadministrated rats and rats bearing antibody to insulin could be monitored.
[Principle]
On the surface of the microplate wells, goat anti rabbit IgG is coated, and when sample is reacted with rabbit anti rat InsC-peptide and biotinylated rat InsCpeptide in the well, the goat antibody captures the complex of the rabbit antibody and rat InsC-peptide formed in competitive reaction. Subsequent reaction with horseradish peroxides (HRP)-conjugated streptavidin to the complex on the well surface results in labeling of the complex with HRP which generates the signal for the presence of rat InsC-peptide in the sample.
[Cross-reactivity]
Specifi c to rat InsC-peptide, but not reactive to either the corresponding of the other animal.


Description Wako Cat. # (Pkg. Size) Dynamic Range Note
Rat C-peptide ELISA Kit 295-57401 (96 tests) 1.56~50 ng/mL Keep and ship at 2~10°C
[Kit Contents]
1. Antibody-coated Microtiter Plate1 plate
2. Rat C-Peptide Standard50 ng
3. Biotinylated Rat C-Peptidefor 8 mL
4. Anti Rat C-Peptide12 mL
5. HRP-conjugated Streptavidin12 mL
6. Chromogen (OPD Tablet)2 tablets
7. Chromogen Diluent Solution24 mL
8. Wash Stock Solution (20 x )50 mL
9. Buffer35 mL
10. Stop Solution (1 mol/L H2SO4)12 mL
11. Adhensive Plate Cover3 covers
page top

C-Peptide High Sensitive (manufactured by Shibayagi)

image
[Features]
1. Rapid Assay (5 hours)
2. Small sample volume (10 μL).
3. Ready-to-Use liquid reagents
4. Excellent precision and reproducibility.

[Cross-reactivity]
Mouse ELISA a Reacts to mouse (100%); rat (90%) and human (95%)
Rat ELISA a Reacts to rat (100%); mouse (75%) and human (85%)

For further information, please visit http://www.shibayagi.co.jp/index-E.htm

Description Wako Cat. # (Pkg. Size) Dynamic Range Note
Mouse C-Peptide ELISA Kit (U Type) 631-07231 (96 tests)
[AKRCP-031]
30 ~ 3,000 pg/mL Keep and ship at 2~8°C
Rat C-Peptide ELISA Kit (U Type) 639-07271 (96 tests)
[AKRCP-030]
30 ~ 3,000 pg/mL
[Kit Contents]
1. Anti-C-peptide-coated plate96 wells (8 x 12)/plate
2. Standard C-peptide solution (6,000 pg/mL)500 μL
3. Buff er Solution60 mL
4. Biotin-conjugated anti-C-peptide100 μL
5. HRP-conjugated streptavidin100 μL
6. Chromogenic substrate reagent (TMB)12 mL
7. Reaction Stopper (1M H2SO4)12 mL
8. Concentrated washing buff er (10 x)100 mL
page top
backindexnext
Wako Product Update Bio-No.2 [ page. 8 ]