1. Kits

1-j) GLP-1

Glucagon-like peptide-1 (GLP-1) is an incretin hormone, which is synthesized in intestinal endocrine cells. This peptide is known to increase insulin secretion by glucose stimulation and suppress glucagon secretion. The kit is applicable to measure rat, mouse and human GLP-1.


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[Features]
1. Sensitivity Dynamic Range : 206 ~ 50,000 pg/mL
2. Reproducibility
. Intra-assay C.V. (%) = 5.4 ~ 6.6
. Inter-assay C.V. (%) = 5.5 ~ 18.9
3. Specifi city
Applicable to measure rat, mouse and human GLP-1.
Little cross-reactivity exists with rat GLP-2, human
GLP-2, human glycentin, and human glucagon.
4. Spike recovery: 89 ~ 110 % (Plasma)
5. Sample volume: 30μL


Description Wako Cat. # (Pkg. Size) Dynamic Range Note
Rat GLP-1 ELISA Kit Wako
(Jaff é method)
291-59201 (96 tests) 206 ~ 50,000 pg/mL Keep at 2~10°C
[Kit Contents]
1. Antibody-coated Microtiter Plate1 plate
2. Rat GLP-1 Standard25 ng
3. Biotinylated Rat GLP-1for 6 mL
4. Anti Rat GLP-1, Rabbit6 mL
5. HRP-conjugated Streptavidin200 μL
6. HRP-conjugated Streptavidin Diluent12 mL
7. Chromogen (OPD Tablet)2 tablets
8. Chromogen Diluent Solution26 mL
9. Wash Stock Solution (20 x)50 mL
10. Buff er10 mL
11. Stop Solution12 mL
12. Adhesive Plate Cover3 pieces
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1-k) GLP-2

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Proglucagon is processed to GLP1 and GLP2 in intestinal L cells. It is reported that GLP-2 is a neurotransmitter involved in the regulation of food intake. The kit is able to measure GLP-2 in rat serum and plasma.


[References]
Tang-Christensen, M. et al. : "The proglucagon-derived peptide, glucagon-like peptide-2, is a neurotransmitter involved in the regulation of food intake", Nature Med., 6, 802-7 (2000).

Description Wako Cat. # (Pkg. Size) Dynamic Range Note
Rat GLP-2 ELISA Kit Wako 292-60601 (96 tests)
0.137 ~ 100 ng/mL Keep at 2~10°C
[Kit Contents]
1. Antibody-coated Microtiter Plate (Anti Rabbit IgG, Goat)1 plate
2. Rat GLP-2 Standard50 ng
3. Biotinylated Rat GLP-21 vial
4. Anti Rat GLP-2, Rabbit6 mL
5. HRP-conjugated Streptavidin12 mL
6. Chromogen (OPD)2 tablets
7. Chromogen Diluent Solution26 mL
8. Wash Stock Solution (20 x)50 mL
9. Buff er25 mL
10. Stop Solution12 mL
11. Adhesive Plate Cover3 covers
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Wako Product Update Bio-No.2 [ page. 10 ]