1. Kits

1-l) Glucose

When a sample is mixed with the Choromogen Reagent, the a-form of glucose in the sample is converted to β-form by mutarotase. β-D-Glucose is oxidized and yields hydrogen peroxide by glucose oxidase (GOD). In the presence of peroxidase (POD), the formed hydrogen peroxide yields a red pigment by quantitative oxidation condensation with phenol and 4-aminoantipyrine. The glucose concentration is obtained by measuring absorbance of the red pigment. [


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[Performance]]
1) Sensitivity (Absorbance):
Purifi ed water: max. 0.03 / 200mg/dL glucose sample: 0.40 ~ 0.55
2)Specifi city (Glucose Concentration):
A known concentration of control serum or urine: within ±12%

[References]
Miwa, I., Okuda, J., Maeda, K. and Okuda, G. : "Mutarotase eff ect on colorimetric determination of blood glucose with -D-glucose oxidase", Clin. Chem. Acta., 37, 538-40(1972).


Description Wako Cat. # (Pkg. Size) Dynamic Range Note
LabAssay™ Glucos
(Mutarotase-GOD method)
298-65701 (1,000 tests) 0 ~ 500 mg/dL Keep and ship at 2~10°C
[Kit Contents]
1. Buff er2 vials x 150 mL
2. Chromogen Reagent2 vials x for 150 mL
3. Glucose Standard I (200 mg/dL Glucose)1 vial x 10 mL
4. Glucose Standard II (500 mg/dL Glucose)1 vial x 10 mL
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1-m) Leptin

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Leptin, secreted from fatty cells, is known as a hormone controlling body fat by suppression of eating and increase of energy metabolism. It is reported, however, that expression of leptin gene in fatty tissue and concentration of leptin in blood are at high levels in obese people and model animals of obesity.
[Principle of the assay]
This kit is a sandwich-format of ELISA with two antibodies specifi c to rat leptin. Monoclonal antibody to leptin is coated on the microplate well, which captures leptin in sample. Following reaction with HRP-conjugated rabbit anti rat leptin antibody, forms HRP labeled antigen-antibody complex on the well, which reports the signal of leptin amount in the sample by chromogenic reaction associated with catalysis of hydrogen peroxide.
Dynamic range :
. Samples for serum and plasma : 312.5 ~ 20,000 pg/mL
. Samples for others than serum and plasma : 78.1 ~ 5,000 pg/mL


[Cross-reactivity]
Specifi c to rat Leptin, but only slightly cross-reactive to human Leptin.


Description Wako Cat. # (Pkg. Size) Dynamic Range Note
Rat Leptin ELISA Kit Wako 297-57601 (96 tests)
Keep at 2~10°C 312.5 ~ 20,000 pg/mL (serum, plasma)
78.1 ~ 5,000 pg/mL (other semples)
[Kit Contents]
1. Antibody-coated Micro Plate (Anti Rat Leptin, mAb)1 plate
2. Rat Leptin Standard20 ng
3. HRP-conjugated Antibody, rabbit6 mL
4. Chromogen (OPD)2 tablets
5. Chromogen Diluent Solution24 mL
6. Wash Stock Solution (20 x)50 mL
7. Buff er A20 mL
8. Buff er B20 mL
9. Stop Solution (1 mol/L H2SO4)12 mL
10. Adhesive Plate Cover2 covers
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Wako Product Update Bio-No.2 [ page. 11 ]