1. Kits

1-n) Insulin

High Level of Insulin (H-Type) [manufactured by Shibayagi]

Determination Kits for High Levels of Insulin from samples such as transgenic mouse or Min-6 are available from Shibayagi. Without diluting the samples, you can measure the insulin value directly. We can offer two kits: for mouse and for rat high-level of insulin.


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[Features]
1. Assay samples: 10μL/well of serum or plasma. Cultured tissue, cells and media
2. Simple assay procedures
3. TMB chromogenic reaction at 450 nm



Description [Shibayagi Code No.] Wako Cat. # (Pkg. Size) Dynamic Range Note
Mouse Insulin ELISA Kit (H-Type) [AKRIN-011H] 634-10379 (96 tests) 0.5 ~ 100 ng/mL Keep at 2~8°C
Rat Insulin ELISA Kit (H-Type) [AKRIN-010H] 637-10629 (96 tests) 0.5 ~ 100 ng/mL
[Kit Contents]
1. Antibody-coated plate96 wells (8 x 12)/plate
2. Standard Insulin solution (200 ng/mL)300 μL/vial
3. Buff er Solution60 mL/vial
4. Biotin-conjugated anti-Insulin200 μL/vial
5. Peroxidase-conjugated streptavidin200 μL/vial
6. Chromogen substrate reagent (TMB)12 mL/vial
7. Reaction Stopper (1M H2SO4)12 mL/vial
8. Concentrated washing buff er (10 x)100 mL/bottle

Insulin Specific (S-Type) [manufactured by Shibayagi]

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This kit is for measurement of mouse insulin with high specifi city and high sensitivity using Sandwich assay principle with least infl uence of co-existing proinsulin. Two kits: for mouse and for rat are available.


[Features]
1. 3 hour rapid assay
2. A small sample volume (rat: 10 μL; mouse: 5 μL)
3. Ready-to-use reagents

[Cross-reactivity]
to proinsulin: less than 5%
to C-peptide: below measurable limits



Description Wako Cat. # (Pkg. Size) [Shibayagi Code #] Dynamic Range Note
Mouse Insulin ELISA Kit (S-Type),
Specific to insulin  [AKRIN-011S]
630-07289 (96 tests) 100 ~ 10,000 pg/mL Keep at 2 ~ 8°C
Rat Insulin ELISA Kit (S-Type),
Specific to insulin  [AKRIN-010S]
631-07199 (96 tests) 78 ~ 5,000 pg/mL
[Kit Contents]
1. Anti-insuline-coated plate96 wells (8 x 12)/plate
2. Standard Insulin solution (5,000 pg/mL)500 μL/vial
3. Buff er Solution60 mL/vial
4. Biotin-conjugated anti-Insulin200 μL/vial
5. Peroxidase-conjugated streptavidin12 mL/vial
6. Chromogen substrate reagent (TMB)12 mL/vial
7. Reaction Stopper (1M H2SO4)12 mL/vial
8. Concentrated washing buff er (10 x )100 mL/bottle
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Wako Product Update Bio-No.2 [ page. 13 ]