A. Wako's Unique Products - 1. Inhibitors

g. Histone Deacetylase (HDAC) Inhibitor

Trichostatin A, 99.0+ % (HPLC)

Wako Cat. No. 200-11993 (1 mg)
<for Biochemistry > Keep at -20°C

HDAC plays a central role in chromatin structure formation associated with the nuclear distribution of DNA. There are presently 17 known types of this enzyme in mammals, which are classifi ed into 3 classes. Also, HDAC Class III has been reported to be associated with regulation of aging and life span.HDAC inhibitors show connections with cell division cycles and differentiation, as well as with antitumor activity and apoptosis-inducing activity through the inhibition of the deacetylating activity of HDAC. They can be used for studies on cellular functions involving histone deacetylase.
Trichostatin A (TSA), a Streptomyces product, specifi cally inhibits the cell cycle of normal rat fibroblasts in the G1 and G2 phases at very low concentrations as reported by Yoshida, et al. TSA-induced G2-arrest induces the formation of proliferative tetraploid cells. In addition, nanomolar concentration of TSA has been shown to cause an accumulation of highly acetylated histones in vivo, and markedly inhibit the activity of partially purifi ed histone deacetylase in vitro.
TSA appears to be a useful product for researching the multiple functions of histone acetylation in regulatory mechanisms of eukaryotic cell proliferation and differentiation.


Source: Streptmyces Hygroscopicus
Solubility: Soluble in ethanol and acetone. 1 mg/10 mL (methanol)
[References]
1) Yoshida, M., Beppu, T.: "Reversible arrest of proliferation of rat 3Y1 fi broblasts in both the G1 and G2 phases by trichostatin A", Exp. Cell. Res., 177, 122-31 (1988)
2) Yoshida, M. et al.: "Potent and specifi c inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A", J. Biol. Chem., 265, 17174-9 (1990)
3) Dion, L.D. et al.: "Amplifi cation of recombinant adenoviral transgene products occurs by inhibition of histone deacetylase", VIROLOGY, 231, 201-9 (1997)
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h. Metastasis Suppressing Agent

MI-22 [Metastasis Inhibitor-22]

Wako Catalog No. 132-15043 (5 mg); 136-15041 (200 mg)
<for Cellbiology> Keep at 2~10°C

Recent studies have shown that the expression of connexin 26, a protein component of gap junction, is increased in cancer cell lines, and suggested that the protein is involved in the mechanism of metastasis in cancer cells. It has been revealed that MI-22 (metastasis inhibitor-22), which is a derivative of oleamide, does not only inhibit the connexin 26-mediated formation of gap junction between cancer and other cells but inhibits the spontaneous metastasis in vivo.


[Features]
1. Oleamide Derivatives
2. Specifi c Inhibition of connexin 26
3. Inhibition of gap junction-mediated intercellular commuications
4. Inhibition of spontaneous metastasis of mouse BL6 melanoma cells
[References]
1) Ito, A., Katoh, F., Kataoka, T. R., Okada, M., Tsubota, N., Asada, H., Yoshikawa, K., Maeda, S., Kitamura, Y., Yamasaki, H. and Nojima, H.: “A role for heterologous gap junctions between melanoma and endothelial cells in metastasis”, J. Clin. Invest., 105, 1189-1197(2000).
2) Ito, A., Morita, N., Miura, D., Koma, Y., Kataoka, T. R., Yamasaki, H., Kitamura, Y., Kita, Y. and Nojima, H.: ”A derivative of oleamide potently inhibits the spontaneous metastasis of mouse melanoma BL6 cells”, Carcinogenesis, 25, 2015-2022(2004).
3) Ohba Y., Kanao Y., Morita N., Fujii E., Hohrai M., Takatsuji M., Hirose H., Miura D., Watari A., Yutsudo M., Zhao H., Yabuta N., Ito A., Kita Y, Nojima H., “Oleamide derivatives suppress the spontaneous metastasis by inhibiting connexin 26”: Int. J. Cancer, 121, 2801-8 (2007)
Description Wako Catalog No. (Pkg. Size) Note
MI-22 [N1,N1,N7,N7-Tetraethyl-2,6-di[(Z)-7-hexadecenyl]-heptanediamide], 93% (HPLC) 132-15043 ( 5 mg)
136-15041 (200 mg)
Filled with inert gas.
Please arrange for immediate use after opening.
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Wako Product Update Bio-No.3 [ page. 6 ]