A. Wako's Unique Products - 2. Fluorescent Bioprobe of PSA
Fluorescent Bioprobe for Visualization of PSA in Living Cells
*: Excitation wavelength and fl uorescence wavelength were not optimized. Please adjust them in accordance with the intended use or the experimental condition based on those wavelengths.
PSA- inhibitory activity IC50 : 4.6 μmol/L (Please see the page 4.)No inhibitory activity toward APN and LAP.
Wako Catalog No. 049-30761 (2 mg)
<for Cellbiology> Keep at RT
C31H32O4N4S=556.68; CAS No. 519183-48-3; λex: 320 nm*; λem: 510 nm*
Puromycin-sensitive aminopeptidase (PSA), which is a neutral aminopeptidase with a substrate specificity similar to that of aminopeptidase N (APN), is distributed mainly in the brain and neurons. Although PSA regarded as an enkephalin-degrading enzyme, the physical roles/functions of PSA remain unclear. PSA is possibly related to cell division and Apoptosis. PSA was reported as a potent modifier of tau-induced pathology and was suggested as a possible tau-degrading enzyme in an unknown mechanism in Alzheimer's disease. PSA activity is inhibited by Puromycin but Puromycin also inhibits APN. DAMPAQ-22, which is structurally modified fluorescent bioprobe of PAQ-22 has been launched. The cellular localization of PSA within the living body could be specifically visualized by the use of DAMPAQ-222).
Visualization of PSA with DAMPAQ-22 in MOLT-4 cells
( These data were provided by Dr. Yuichi Hashimoto, Institute of Molecular and Cellular Biosciences, The University of Tokyo)
Related Wako Products:
- PAQ-22 / DAMPAQ-22 ⇒ leaflet