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TLC Plate for Thin Layer Chromatography
Silicagel 70 F254 TLC Plate-Wako

Silicagel 70 F254 TLC Plate-Wako is coated with porous silica gel having uniform particles in pore diameter 7 nm (70 Å) uniformly on its glass plate. With the addition of green fluorescent substances, the separated spots are observed as dark spots in the green background by irradiation of ultraviolet (254 nm).


1. Feature

  • Using silica gel of uniform particles diameter and having a high separability.

Physical Property Information

Particle diameter of silica gel 5 - 15 μm
Pore diameter of silica gel 7 nm (70 Å)
Specific surface area of silica gel 450 m2/g
Pore volume of silica gel 0.8 mL/g
Thickness of silica gel layer 230 - 250 μm
Support Glass plate
Fluorescent indicator Monochromatic fluorescent substance [254nm]

2. Experimental Example

1) Development of standard samples

Experimental conditions
[Sample]
    1. Caffeine
    2. Naproxen
    3. p- Acetamidophenol
    4. p-hydroxybenzoate
[Mobile Phase]
    Chloroform : methanol =
      a) 95:5   b) 90:10
      c) 85:15   d) 80:20
[Detection]       UV 254 nm
[Deployment Distance] 10 cm

2) Development of crude drug standards

Experimental conditions
[Sample] Saikosaponin a
[Mobile Phase]
            Ethyl acetate: ethanol: water = 8: 2: 1
[Coloration Reagent]
      Sprayed with 4-dimethylamino benzaldehyde reagent,
      then heated at 105°C for 5 minutes.
[Deployment Distance] 10 cm

      ※ Japanese Pharmacopoeia 16th Edition
         "Bupleurum Root" confirmation test (2)

3. Product List

Product Name Layer Thickness Package Size Wako Cat. No.
Silicagel 70 F254 TLC Plate-Wako 0.25 mm 10 plates (5 x 10 cm) 199-17813
25 plates (20 x 20 cm) 193-17811
100 plates (5 x 20 cm) 197-17814
200 plates (5 x 10 cm) 193-17816

4. Related Products

4-1. Silicagel TLC plates

Preparative TLC and aminopropylated silica gel TLC are also available.

Product Name Layer Thickness Package Size Wako Cat. No.
Silicagel 70 Plate-Wako
(Fluorescent agent-free type)
0.25 mm 10 plates (5 x 10 cm) 193-08381
100 plates (5 x 20 cm) 197-08384
25 plates (20 x 20 cm) 199-08383
Silicagel 70 FM Plate-Wako
(Coping with wide-area UV-250 ~ 400nm )
10 plates (5 x 10 cm) 190-08391
100 plates (5 x 20 cm) 194-08394
25 plates (20 x 20 cm) 196-08393
Silicagel 70 PF254 Plate-Wako
(Preparative TLC : Thick layer type)
0.75 mm 10 plates (20 x 20 cm) 195-12871


4-2. Amino silica gel (NH2) TLC plate

Product Name Layer Thickness Package Size Wako Cat. No.
NH2 Silica Gel 60 F254 Plate-Wako 0.25 mm 25 plates (20 x 20 cm) 146-08631
100 plates (2.5 x 6.6 cm) 143-08641
0.5 mm 10 plates (20 x 20 cm) 149-08621
0.75 mm 10 plates (20 x 20 cm) 145-08721

Chromato Sheet 2 4-3. Paper sheet for TLC Chromato Sheet II

Chromato sheet II is a sheet for thin layer chromatography, which is made from "paper" fiber coated with the silica gel and fluorescent agent F254.
Having similar separation property and reproducibility to TLC plate, it also can be easily cut with scissors for the support of "paper", lightly, not bulky even at the time of storage.
[Size: 20 x 20 (cm), Thickness: Approx. 0.3 mm, Weight: Approx. 7 g/sheet]


Product Name Thickness Package Size Wako Cat. No.
Chromato Sheet II Approx. 0.3 mm 25 sheets (20 x 20 cm) 038-24061
Home > Products > Chemicals > Others > Silicagel 70F254 TLC Plate-Wako
Home > Products > Analysis > Chromatography > Silicagel 70F254 TLC Plate-Wako