Wako Pure Chemical Industries,Laboratory Chemicals
WAKODistributors WAKO Wako USA WAKOWako GmbH WAKOContact Us

Online Catalog | Product Information | Ordering Information | SDS | Certification of Analysis | Catalog Request |
Home > Products > Chemicals > SanCat-R

Secondary alcohol dehydrogenase

SanCat-R




This enzyme selectively oxidates (R)-secondary alcohol in a racemic mixture into ketones. It undergoes efficient enzymatic conversion in open system in water/solvent at 40°C. This product utilizes Heme-Binding Protein (HBP) derived from green pea.

INDEX

  1. Features
  2. Comparison with conventional method
  3. Protocol
  4. Product List
  5. Examples of reaction and analysis
  6. References
Images


1. Features



2. Comparison with conventional method



3. Protocol

Protocol used

  1. Add a stirrer into a 18 mm x 15 mL test tube.
  2. Add 30 mg of SanCat-R into the tube.
  3. Add 4 mL of distilled water.
  4. Add 30 μL of 1.2 mM DMSO substrate solution, and incubate at 40°C, with stirring (700 rpm).
    * Reaction ends when all R bodies are oxidated into ketones. After reaction ended, remove SanCat-R by filtration or centrifugation.


4. Product List

Product Name Package Size Wako Catalog No. Manufacturer Storage Condition
    SanCat-R 1 g 355-34211 Wako Chemical, Ltd. Keep at 2-10°C
5 g 351-34213

* This product is developed by SANYO FOODS Co., Ltd.



5. Examples of reaction and analysis

Examples of reaction and analysis




6. References

  1. Giles, M. N. et al.: Biochem. Biophys. Res. Commun., 300, 1 (2003).
  2. Giles, M. N. et al.: Chem. Biol., 10, 677 (2003).
  3. Egami, H. et al.: J. Am. Chem. Soc., 129, 8940 (2007).
  4. Nagaoka, H. et al.: Biotechnol. Prog., 28, 953 (2012).
  5. Nagaoka, H. et al.: Biosci. Biotechnol. Biochem., 63, 1991 (1999) .
  6. Nagaoka, H. et al.: Biosci. Biotechnol. Biochem., 64, 781 (2000).
  7. Nagaoka, H. et al.: Biosci. Biotechnol. Biochem., 65, 634 (2001).
  8. Nagaoka, H.: Biotechnol. Prog.: 19, 1149 (2003).
  9. Nagaoka, H.: Biotechnol. Prog., 20, 128 (2004).
  10. Wako Junyaku Jiho: 71(4), 1 (2003).

QUOTATION REQUEST
PAGE TOP
Note
Home > Products > Chemicals > Lithium cation endohedral fullerene > SanCat-R