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NH Immunochromato Series

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Simple, rapid detection kit for food poisoning bacteria
NH Immunochromato Series



"NH Immunochromato Series" is a kit to detect food poisoning bacteria by immunochromatography.
Use in combination with the conventional culture method is expected to reduce time and cost.

<<Measurement items>>
- Enterohemorrhagic E. coli O157
- Enterohemorrhagic E. coli O26
- Enterohemorrhagic E. coli O111
- Risteria
- Salmonella
- Campylobacter
- Verotoxin (VT1, VT2)

 

Features
- Low cost
it is the cheapest immunochromatography kit commercially available now.
Compared to the culture method, reduction of cost is expected including manpower cost.

- Simple operation
The operation is simple just to drop an enrichment medium on a test strip.

- Easy and quick assessment
Assessment is simple and rapid by checking a red-purple line after 15 minutes compared to the conventional culture method.

- Extensive line-up
Simple detection kits for E. coli O26 and O111 are the world's first kits.
The verotoxin detection kit can detect VT1 and VT2 separately.


Test procedure and comparison with the culture method (in the case of E. coli O157)



Test method for O111 (possible to perform the test together with O157 with a minimum trouble)
While a test for enterohemorrhagic E. coli O157 takes 4 days from preparation of a sample to various identifications when it is performed on the basis of an official method, it can be detected in 2 days by the use of immunochromatography (*).
O111 can be identified simultaneously using a sample prepared for testing O157 by the addition of a test strip for O111 (see Fig. 1).
NH Immunochromato Series that allows testing by a simple operation in a short time is suited to screening.
* An identification test by another method is required when a positive result is obtained by the immunochromatography.

Detection of verotoxins (VT1, VT2) (#302-93321 NH Immunochromato VT1/2 is used)

(i): The result is judged as negative when a red-purple line is observed only at the control line appearance position without observation of a red-purple line at the test line appearance position.
(ii)-1: The result is judged as VT1- and VT2-positive when red-purple lines are observed at the VT1 test line appearance position, VT2 test line appearance position and the control line appearance position after 15 minutes. (ii)-2: The result is judged as VT2-positive when red-purple lines are observed at the VT2 test line appearance position and the control line appearance position after 15 minutes.
(ii)-3: The result is judged as VT1-positive when red-purple lines are observed at the VT1 test line appearance position and the control line appearance position after 15 minutes.
(iii): A re-test should be performed if a red-purple line is not observed at the control line appearance position regardless whether a test line is observed or not.

Product List

Wako Catalog No. Product Name Detection sensitivity *1 Packaging
304-31361 NH Immunochromato O157 1 x 104 - 1 x 106 CFU/mL For 20 tests
304-34421 NH Immunochromato O26 1 x 104 - 1 x 106 CFU/mL For 20 tests
301-34431 NH Immunochromato O111 1 x 104 - 1 x 106 CFU/mL For 20 tests
300-31581 NH Immunochromato Risteria 1 x 105 - 1 x 107 CFU/mL For 20 tests
303-31691 NH Immunochromato Salmonella 1 x 105 - 1 x 107 CFU/mL For 20 tests
301-83141 NH Immunochromato Campylobacter 1 x 104 - 1 x 106 CFU/mL *2 For 20 tests
302-93321 NH Immunochromato VT1/2 2.5 ng/mL For 20 tests

*1 The detection sensitivity may vary depending on the ingredients in a sample.
*2 The test was performed using C. jejuni reference culture.


Related products (NH beads series)


These are magnetic beads for collection of enterohemorrhagic E. coli O157, O26 and O111, which are the causative bacteria for food poisoning. Small packages for 25 tests allow to use all up without wasting.

Wako Catalog No. Product Name Packaging
300-85671 NH beads O157 For 25 tests
307-85681 NH beads O26 For 25 tests
304-85691 NH beads O111 For 25 tests
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