Analytical Chemistry

for effective trapping of variety of metal elements

Presep® PolyChelate (250mg/3mL)

Wako Catalog No.296-33491i5 x 10 syringesj

Presep ® PolyChelate is …

Presep ® PolyChelate, which is a solid-phase extraction cartridge contains a unique chelate resin codified with carboxymethylated polyethylenimine. A variety of metal elements can be trapped much more effectively with this cartridge than an ordinal IDA (Iminodiacetic acid) type chelate resin. In addition, metal oxo-acids (Mo, V, W) can be trapped under acidic or neutral conditions without any interruptions of alkali metals and alkaline-earth metals.

An Example of Procedure

Column Conditioning
(1) CH3OH:  5 mL
(2) 3M HNO3: 10 mL
(3) H2O: 20 mL
(4) 0.1M CH3COONH4: 10 mL

Solid Phase Extraction
pH adjustment of sample
↓ (1) 0.1M CH3COONH4 5mL/100mL of sample
↓ (2) pH adjustment using HNO3 or NH3 solution
Sample Loading: ~ 500 mL
↓ Flow rate: 3 ~ 10 mL/min.
Washing
↓ H2O: 20 mL
Elution
↓ 1 ~ 3 M HNO3: 3 ~ 5 mL
Instrumental Analysis  (ICP-AES, ICP-MS, AAS, etc.)

Effect on pH on recovery

Effect on pH on the recoveries of various trace elements using Presep ® PolyChelate
Sample volume: 100 mL; each element: 10 µg; Flow rate: 3 mL/min; Elution: 3 mL of 3M HNO3
Measurement: PerkinElmer Optima 3000 DV ICP-AES, cross-flow nebulizer and a Scott-type spray chamber

figure

Data was provided from Kagaya, engineering dept., Toyama University. Since these data show an example of the actual use, no warranty is given as to the specification of the product.

Measurement

ElementsTreated wastewater *1 (n=3) Surface seawater *2 (n=3)
Found/µg/LRecovery *3/% Found/µg/LRecovery *3/%
Cd0.2 ± 0.0293 ± 2.9(0.03 ± 0.004)96 ± 1.7
Co< 0.03392 ± 3.7(0.02 ± 0.012)96 ± 1.5
Cu1.1 ± 0.13104 ± 3.50.77 ± 0.017112 ± 3.2
Fe33 ± 3.3114 ± 13.81.3 ± 0.0594 ± 3.2
Mn871 ± 7564 ± 9.0Not measureNot measure
Mo458 ± 9.398 ± 1.77.4 ± 0.07100 ± 4.5
Ni11 ± 0.2101 ± 4.90.46 ± 0.017100 ± 1.8
Pb< 0.2294 ± 2.70.09 ± 0.052104 ± 2.0
V< 0.2292 ± 3.11.3 ± 0.0596 ± 2.5
Zn11 ± 3.183 ± 2.12.5 ± 0.24102 ± 2.2

Products

DescriptionWako Catalog No. (Pkg Size)
Presep ® PolyChelate (250 mg/3mL) (for Sample Pretreatment)296-33491 (5 x 10 syringes)
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