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Wakopak® Navi Series

Packed Columns for HPLC

The Wakopak ® Navi series is a lineup of three types of packed columns containing different alkyl chains and is intended for reversed-phase HPLC. These highly-efficient columns contain high-purity silica gel particles with a diameter of 5 μm, which are modified with an octadecyl group (C18), docosyl group (C22) or triacontyl group (C30), end capped, and filled using our unique technology. Use of C22 and C30 has enabled analyses of speciments that were difficult to separate with ODS (C18), and has thus expanded the range of applications.

Wakopak Navi Columns


INDEX

1. Features
2. Wakopak® Navi C18-5
3. Wakopak® Navi C22-5
4. Wakopak® Navi C30-5
5. Basic retention capacity
6. Column List
6-1. Semi-Micro Columns
6-2. Analysis Columns
6-3. Separation Columns


1. Features

High-purity silica gel
   Highly purified silica gel, 5 μm in particle diameter, is used.

End-capping
  The products are end-capped to make the characteristics of high-purity silica gel more effective.

Expanded range of application
  Three types of columns offer separation capacities of their own.

Filler
Feature
Separation Mode
Particle Size
End-capping
Navi C18-5
Navi C18-5 is widely applicable to basic acidic and coordination compounds
reversed-phase partition chromatography
5 μm
well-done
Navi C22-5
By using a mobile phase with high water content, purification and retention characteristics higher than those of C18 can be obtained.
Navi C30-5
C30 shows an outstanding ability to recognize structures.
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2. Wakopak® Navi C18-5
Development for LC/MS, this ODS column comprises of an inner glass-lined stainless steel tube and is maximally inactivated. It uses a high-purity titanium frit at the inlets and outles exposed to specimens to minimize nonspecific adsorption.

First choice for analysis with a wide application range
This is ODS filler upgraded further by improving the modification method of octadesyl group (C18) and thorough end-capping.
Its application range is wide and it is best suited as a first choice column.

Examples of analyses

Column: Navi C18-5, 4.6 mm φ x 250 mm
Mobile phase: A mixture of CH3CN and 10 mM KH2PO4 (pH=7.0) in 30:70 (v/v)
Flow rate: 1.0 mL/min. at 35°C
Detection: UV 254 nm, 0.16 Aufs
Samples:
  1) Uridine (0.02 mg)
  2) Procainamide HCl (0.05 mg)
  3) Phenol (0.2 mg)
  4) 2,4,6-Trimethylpyridine (0.1 μL)
  5) Methyl benzoate (0.5 μL)
in 1 mL CH3CN/H2O=30/70, 10 μL injection


Column: Navi C18-5, 4.6 mm φ X 250 mm
Mobile phase: A mixture of CH3CN and 50 mM KH2PO4 (pH=3.5), 0.1% EDTAï½¥2Na = 60/40 (v/v)
Flow rate: 1.0 mL/min. at 35°C
Detection: UV 240 nm, 0.64 Aufs
Samples:
  1) Oxine-Cu (0.05 mg/mL CH3OH) 5μL injection

⇒ Column List
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3. Wakopak® Navi C22-5
Manifests power for separation of highly polarized compounds.
Filled with monomeric silica gel end-capped after chemical modification with docosyl group (C22), this column can be used in a mobile phase with high water content and has higher retention and separation characteristics than ODS, even for compounds with high polarity.
Its higher separation and retention characteristics can be obtained by using a mobile phase with high water content.


Examples of analyses

Column: Navi C22-5 4.6mmφ x 250 mm
Mobile phase: 50 mM NaH2PO4 (pH = 2.4)
Flow rate: 1.0 mL/min. at 40°C
Detection: UV 235 nm, 0.04 Aufs
Samples:
  1) NE (10 μg/mL)
  2) E (10 μg/mL)
  3) DHBA (20 μg/mL)
  4) DA (30 μg/mL)
  5) DOPA (30 μg/mL)
5 μL injection



Column:Navi C22-5 4.6mmφ x 250 mm
Mobile phase:20 mM NaH2PO4 (pH = 2.5)
Flow rate:1.0 mL/min. at 35°C
Detection:UV 210 nm, 0.04 Aufs
Samples:
  1) Oxalic Acid (0.02 mg)
  2) Tartaric Acid (0.1 mg)
  3) Formic Acid (0.2 mg)
  4) Lactic Acid (0.55 mg)
  5) Acetic Acid (0.5 mg)
  6) Citric Acid (0.35 mg)
  7) Fumaric Acid (0.003 mg)
  8) Succinic Acid (0.8 mg)
  9) Acrylic Acid (0.014 mg)
  10) Propionic Acid (1.2 mg)
  11) Levulinic Acid (2.0 mg)
10 μL injection
Other HPLC Applications
  1. Analysis of triacylglycerols (TAG) in Palm Oil
  2. Analysis of triacylglycerols (TAG) in Canola Oil

⇒ Column List
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4. Wakopak® Navi C30-5
High ability to recognize structures is best suited to analysis of congeners
Filled with polymeric silica gel end-capped after chemical modification with triacontyl group (C30)with longer alkyl chains into polymeric form, this column provides higher stereoselectivity (plane recognition ability) and produces different results of analyses from those obtained with ODS.
The outstanding ability of triacontyl group to recognize structures is best suited to analyses of congeners. This column may exhibit peculiar behaviors such as reversion of elution order based on the polymeric form.

Examples of analyses


Column:Navi C30-5 (4.6 mmφ x 250 mm)
Mobile phase:CH3CN/CH3OH/CHCl3 = 75/10/15 (v/v/v)
Flow rate:2.0ml/min. at 35°C
Detection:UV470nm, 0.16 Aufs
Samples:
  1) α-Carotene (0.0 5mg/mL)
  2) β-Carotene (0.05 mg/mL)
 5 μL injection


Column:Navi C30-5 (4.6 mmφ x 250 mm)
Mobile phase:CH3CN (100%)
Flow rate:1.0ml/min. at 35°C
Detection:UV295nm, 0.064 Aufs
Samples:
  1) D-δ-Tocopherol (0.1 mg/mL)
  2) D-γ-Tocopherol (0.1 mg/mL)
  3) D-β-Tocopherol (0.1 mg/mL)
  4) D-α-Tocopherol (0.1 mg/mL)
10 μL injection
HPLC Applications
  1. Analysis of triacylglycerols (TAG) in Palm Oil
  2. Analysis of triacylglycerols (TAG) in Canola Oil

⇒ Column List
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5. Basic retention capacity
 

Column: CH3CN/H2O = 60/40 (v/v)
Mobile phase: 1.0 mL/min. at 35°C
Flow rate: UV 254 nm, 0.16 Aufs
Samples:
  1) Uracil (7.75 μg/mL)
  2) Benzene (1.45 μL/ml)
  3) Naphthalene (0.2 mg/mL)
6 μL injection




6.Column List
 6-1. Semi-Micro Columns
  Column size Joint Type Wako Catalog No.
Navi C18-5 Navi C22-5 Navi C30-5
Semi-micro column 1.0φ x 150 mm DuPont inquire inquire inquire
Waters inquire inquire 234-61003
2.0φ x 150 mm DuPont 234-60361 inquire inquire
Waters 230-60363 232-60703 232-61043
2.0φ x 250 mm DuPont 231-60371 233-60711 inquire
Waters 237-60373 239-60713 239-61053
Precolumn
for Semi-micro column
2.0φ x 50 mm DuPont inquire inquire inquire
Waters 233-60353 inquire inquire

QUOTATION REQUEST for Waopak® Navi  PAGE TOP
 6-2. Analysis Columns
  Column size Joint Type Wako Catalog No.
Navi C18-5 Navi C22-5 Navi C30-5
Analysis Column 3.0φ x 150 mm DuPont inquire inquire inquire
Waters 231-60393 inquire 233-61073
3.0φ x 250 mm DuPont inquire inquire 234-61081
Waters 234-60403 230-60743 230-61083
4.0φ x 150 mm DuPont 239-60431 inquire inquire
Waters 235-60433 231-60773 237-61113
4.0φ x 250 mm DuPont 233-60451 inquire 235-61131
Waters 239-60453 235-60793 231-61133
4.0φ x 300 mm DuPont inquire inquire inquire
Waters inquire inquire inquire
4.6φ x 100 mm DuPont 234-60501 inquire inquire
Waters 230-60503 inquire 236-61183
4.6φ x 150 mm DuPont 231-60511 237-60851 237-61191
Waters 237-60513 233-60853 233-61193
4.6φ x 200 mm DuPont inquire inquire inquire
Waters 234-60523 inquire 236-61203
4.6φ x 250 mm DuPont 235-60531 231-60871 237-61211
Waters 231-60533 237-60873 233-61213
6.0φ x 150 mm DuPont 236-60561 inquire inquire
Waters 232-60563 234-60903 234-61243
6.0φ x 250 mm DuPont inquire inquire inquire
Waters inquire 231-60913 231-61253
Pre-Column
for Analysis Column
Column size Joint Type Navi C18-5 Navi C22-5 Navi C30-5
3.0φ x 30 mm DuPont inquire inquire inquire
Waters 234-60383 inquire inquire
4.0φ x 10 mm DuPont inquire inquire inquire
Waters 231-60413 inquire inquire
4.6φ x 10 mm DuPont 237-60471 inquire inquire
Waters 233-60473 235-60813 235-61153
4.6φ x 30 mm DuPont 234-60481 inquire 236-61161
Waters 230-60483 232-60823 232-61163
4.6φ x 50 mm DuPont inquire inquire 233-61171
Waters 237-60493 inquire 239-61173

QUOTATION REQUEST for Waopak® Navi  PAGE TOP

 6-3. Separation Columns
  Column size Joint Type Wako Catalog No.
Navi C18-5 Navi C22-5 Navi C30-5
Separation column 10φ x 250 mm DuPont inquire inquire inquire
Waters 230-60623 236-60963 232-61303
10φ x 300 mm DuPont inquire inquire inquire
Waters inquire inquire inquire
20φ x 250 mm DuPont inquire inquire 237-61331
Waters 231-60653 237-60993 233-61333
Pre-column
for Separation Column
10φ x 50 mm DuPont inquire inquire inquire
Waters 233-60613 inquire 239-61293
20φ x 50 mm DuPont inquire inquire inquire
Waters 234-60643 inquire 236-61323

QUOTATION REQUEST for Waopak® Navi  PAGE TOP


Note

  • Listed products are intended for laboratory research use only, and not to be used for drug, food or human.
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