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Wako FAQ -Chromatography- Q2


FAQ
Q2: Is there any simple method to change the solvent concentrations in the mobile phase once prepared?



You can change the concentrations easily by substituting values in the following diagrams.

(N) Concentration (%) of the solvent to be changed

(A) Concentration (%) of the solvent in the mobile phase
      currently in stock

(B) Concentration (%) of the solvent to be used in the preparation

(a) Necessary volume of the mobile phase (A)
     (numerical difference between (N) and (B))

(b) Necessary volume of the solution phase (B)
     (numerical difference between (N) and (A))


Example 1) To change the mobile phase of 30% CH3OH to that of 50% CH3OH using 70% CH3OH solution
Mobile phase of 30% CH3OH: 20 Mix in these proportions.
50% CH3OH solution: 20

Example 2) To change the mobile phase of 45% CH3CN to that of 25% CH3CN using water.
Mobile phase of 45% CH3CN : 25 Mix in these proportions.
Water :20

INDEX
Q1Which reference standard do you recommend for use to check performance of a column?
Q2Is there any simple method to change the solvent concentrations in the mobile phase once prepared?
Q3How are ODS columns, Wakosil-II HG, AR and RS, used in different situations?
Q4Ion pair reagents for low wavelength are on the market. What effects and differences do they show with actual samples?
Q5We wish to fractionate by HPLC. How can we determine the optimal separating conditions?
Q6We wish to perform fractionation by the reverse phase flash chromatography. How can we determine the optimal separating conditions?
Q7Is column performance improved by narrowing the inside diameter of a column?
Q8We are examining mobile phase conditions of HPLC.We intend to examine by changing solvents.Is there any method to estimate the elution profile in advance?
Q9We are examining the conditions of a mobile phase in the HPLC. We would like to know how to regulate retention of a sample that has a readily dissociating functional group.
Q10I have used a semi-microcolumn (2φ mm) but cannot obtain improvement of sensitivity generally offered.
I wonder what is the cause as I am sure to have used the system applicable to microcolumns.
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