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Wako FAQ -Chromatography- Q3

Q3: How are ODS columns, Wakosil-II HG, AR and RS, used in different situations?

The columns in Wakosil-II series are synthesized using highly pure spherical silica gel as a raw material and end-capped to a high degree. Thus, they offer peak shapes with excellent symmetry in the analysis of not only acidic and basic compounds but also metal coordination compounds. Three types of ODS columns, Wakosil-II, AR and RS, with different separation characteristics are available in Wakosil-II series and effective uses utilizing respective features are shown in the following.
•Applicable to wide pH range and, thus, best suited to separation of proteins and peptides.
•Suited to separation of polyaromatic compounds due to presence of spatial cognition ability.
•Most popular high-performance monomeric ODS type suited to separation of a wide range of compounds.
•Effective as a first choice for examination of analysis conditions.
•Designed to retain polar compounds longer to best suit to analysis of biological components and water-soluble compounds.
•Suited particularly to separation of basic compounds due to the highest end-capping efficiency.

We also offer a screening kit with these 3 types of different characteristics. Please utilize it for examination of operating conditions.

Q1Which reference standard do you recommend for use to check performance of a column?
Q2Is there any simple method to change the solvent concentrations in the mobile phase once prepared?
Q3How are ODS columns, Wakosil-II HG, AR and RS, used in different situations?
Q4Ion pair reagents for low wavelength are on the market. What effects and differences do they show with actual samples?
Q5We wish to fractionate by HPLC. How can we determine the optimal separating conditions?
Q6We wish to perform fractionation by the reverse phase flash chromatography. How can we determine the optimal separating conditions?
Q7Is column performance improved by narrowing the inside diameter of a column?
Q8We are examining mobile phase conditions of HPLC.We intend to examine by changing solvents.Is there any method to estimate the elution profile in advance?
Q9We are examining the conditions of a mobile phase in the HPLC. We would like to know how to regulate retention of a sample that has a readily dissociating functional group.
Q10I have used a semi-microcolumn (2φ mm) but cannot obtain improvement of sensitivity generally offered.
I wonder what is the cause as I am sure to have used the system applicable to microcolumns.
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