Refer to pp.92-95A-34-36
These are solid-phase extraction cartridges for quickly and easily pretreating pesticide residues using styrene divinylbenzene polymethacrylate resin as an adsorbing material.
The product comes in two types of cartridges; Presep®-C Agri (Short) in cartridges with closed ends and Presep® Agri in syringe type cartridges. The user can use the type appropriate to the pretreatment operation method, pressurization or evacuation.

1. The hydrophobic polymer gel having hydrophilic property is effective in concentration of trace hydrophobic components in water.
2. High recovery of pesticides, such as high-polarity and metal coordination components (asulam and oxine-copper), which are difficult to recover
3. Presep®-C Agri (Short) is applicable to concentration and recovery by a constant flow pressure pump.
4. In HPLC analysis, the quantities of various kinds of pesticides can be quickly and easily determined in accordance with official analytical methods by combining with Wakopak® WS Agri-9 and special eluent.


Column:Presep®-C Agri (Short)
Column conditioning: ① CH3CN 10 mL
              ② CH3OH 10 mL
              ③ H2O  10 mL
   ↓
Feed of sample water※1:500 mL
   ↓
Cleaning:H2O 5 mL
   ↓
Elution:CH3CN 2 mL※2
   ↓
Concentration: Under N2 flow (approx. 0.2 to 0.3 mL)
   ↓
Re-dissolution: Dilution to 1 mL with H2O
   ↓
Injection of 10 μL to HPLC-UV

※1 500 mL of sample water: 10 mL of 0.2mol/L EDTA・2Na is added.Pesticide reference standard is added.
※2: Amount of eluate: 5 mL by official analytical method


Column :Wakopak® MS-Agri-9 GT(2.0 mm × 150 mm)
Eluent :A)10 mM CH3COONH4 (pH3.7)
     B)CH3CN
        0-10 min B:12-70 %
        10-13 min B:100 %
        13-23 min B:12 %
Flow Rate :0.2 mL/min. at 40°C
Injection Vol.: 1 ppm, 2 μL


ESI, MRM
IonSpray Voltage: 5500 V (pos)   -4500 V (neg)
Temperature: 700°C300°C
Curtein Gas : 4020
Collison Gas : 5 3
Ion Source Gas1 :80 40
Ion Source Gas2 : 70 80
System : 3200 Q TRAP (ABI)


18 components in pos mode
Pesticide
No.
Sample Name Q1/Q3 RT
(min)
Recovery
(%)
74 Methomyl 163.1/88.1
amu
4.4 50.8
(85.2)
71 MPP oxon sulfoxlde 279.0/104.2
amu
5.8 101.6
87 Tricyclazole 190.0/163.2
amu
6.9 99.1
71 MPP oxon sulfone 295.0/217.1
amu
7.2 99.7
18 Carbofuran
(Carbosulfan metabolite)
222.1/165.2
amu
8.5 103.1
96 Thlodlcard 355.1/88.1
amu
8.6 99.6
71 MPP sulfoxlde 294.9/109.0
amu
9.0 100.1
1 Thluram 240.9/88.1
amu
9.5 94.3
82 Probenazole 224.0/130.2
amu
9.5 98.6
71 MPP oxon 263.0/231.1
amu
9.6 99.5
86 Bensulfuron-methyl 411.0/149.2
amu
10.0 100.7
95 Flazasulfuron 408.2/182.1
amu
10.1 98.6
71 MPP sulfone 310.9/124.9
amu
10.4 99.2
98 Slduron peak1 233.1/137.2
amu
10.4 99.7
98 Slduron peak2 - 10.6 98.3
90 Azoxystrobin 404.1/372.1
amu
10.8 99.6
84 Dymron 269.1/151.1
amu
11.1 99.6
71 MPP 279.0/169.0
amu
12.1 104.3
58 Carproamid 336.0/139.2
amu
12.2 101.8
※The methomyl recovery in parentheses was obtained when Presep-Agri was used. ※Siduron: 2 peaks were detected.
-
:Components added to simultaneous analytical testspecified in “Attached method 18” issued in April, 2008
10 components in neg mode
Pesticide
No.
Sample Name Q1/Q3 RT
(min)
Recovery
(%)
36 Asulam 228.9/196.7
amu
5.7 99.6
17 Bentazone 239.1/131.6
amu
6.8 99.3
19 2,4-PA 219.9/161.7
amu
7.5 99.1
20 Triclopyr 255.9/198.1
amu
8.0 101.2
45 MCPP(Mecoprop) 213.0/140.9
amu
8.8 99.9
94 Halosulfuron-methyl 433.6/252.2
amu
9.6 99.1
68 DCMU(Diuron) 232.0/186.9
amu
9.7 100.4
26 Iprodione 329.9/245.1
amu
11.4 98.9
42 Bensulide(SAP) 395.9/212.7
amu
12.3 96.7
102 Fipronil 436.8/330.1
amu
12.4 97.3

10 components in neg mode
Pesticide
No.
Sample Name Recovery
(%)
28 Oxine-copper 96.2
48 Carbary(l NAC) 101.0
75 MBC: Benomyl decomposition product 97.5
※These components were excluded because they were unstable when mixed.
※The recovery values are measurements obtained by HPLC-UV.


Description Wako Cat. No. (Pkg. Size)
Presep®-C Agri (Short) 296-32651   (10 units x 5)
Presep® Agri 291-26851   ( 50 units)




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