Wakopak® Wakosil-DNA
Analysis of oligodeoxyadenosine (12 to 30 mer)

This column was developed for analysis and fractionation of oligonucleotide. It ensures efficient analysis and fractionation of synthesized ss DNA (single-stranded DNA) of up to several tens of mer differing by 1 mer. Although it is said that the performance of oligonucleotide analysis columns depends on the quality of raw material silica gel, Wakosil-DNA Column uses carefully-screened silica gel and shows high recovery. Each lot of the packing material is subjected to oligo DNA analysis test to assure the quality.

Column :Wakopak® Wakosil DNA(4.6 mm × 150 mm)
Eluent :A)0.1 M TEAA(pH7.0)
 B)CH3CN/0.1 M TEAA*(pH7.0)=50/50(v/v)
linear gradient 0→60 min. B 12→20%
Flow Rate :1.0 mL/min. at R.T.
Detection :UV 260 nm

*TEAA Triethylamine-Acetate Buffer

Purification of synthesized DNA (19 mer)
HPLC columns
Product Name Size Specification Volume Wako Cat. No.
Wakopak® Wakosil-DNA 4.6 mm × 150 mm D 1 pcs. 239-59321
4.6 mm × 250 mm D 1 pcs. 236-59331