Columns for biological sample pretreatment : Column-switching analysis

Wakopak® Wakosil GP-N6
Analysis of drugs in serum by column-switching method

(Pretreatment conditions)

Column :Wakopak® Wakosil GP-N6(4.6 mm × 50 mm)
Eluent :CH3CN/0.2M NaH2PO4, Na2HPO4(pH6.0)=2/98(v/v)
Flow Rate :1.0 mL/min. at 30°C

(Analytical conditions)

Column :Wakopak® Wakosil-II5C18 RS(4.6 mm × 50 mm)
Eluent : :CH3CN/0.1M NaH2PO4, Na2HPO4(pH6.0)=30/70(v/v)
Flow Rate :1.0 mL/min. at 30°C
Detection :UV 254 nm 0.032AUFS
Column Switching Time :4.8-6 min.
Sample :Human Serum 20 μL
1)Phenobarbital 20 μɡ/mL
2)Carbamazepine 5 μɡ/mL
3)Phenytoin 40 μɡ/mL
Recovery of drugs
Drug Concentration in serum (μɡ/mL) Recovery (%) CV (%)
Phenobarbital 20 99.3 1.06
Carbamazepine 5 96.5 1.12
Phenytoin 40 102.3 0.78
Product Name Size Joint Type Package Size Wako Cat. No.
Wakopak® Wakosil GP-N6 4.6 mm × 30 mm W 1 unit 231-59423
D 1 unit 235-59421
4.6 mm × 50 mm W 1 unit 238-59433
D 1 unit 232-59431
4.6 mm × 150 mm W 1 unit 232-59453
D 1 unit 236-59451
4.6 mm × 250 mm W 1 unit 236-59473
D 1 unit 230-59471
6.0 mm × 150 mm W 1 unit 230-59493